{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T18:22:19Z","timestamp":1785435739372,"version":"3.56.0"},"reference-count":41,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T00:00:00Z","timestamp":1780358400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T00:00:00Z","timestamp":1780358400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2026,6,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Functional constipation (FC) is a common gastrointestinal disorder that can seriously affect patients\u2019 quality of life. Probiotics have emerged as a promising strategy for preventing and managing gastrointestinal diseases. Previously, we isolated\n                      <jats:italic toggle=\"yes\">Lactobacillus acidophilus<\/jats:italic>\n                      C4 from the feces of a healthy child, and here, we investigated its probiotic efficacy against FC in a mouse model. Prophylactic treatment with\n                      <jats:italic toggle=\"yes\">L. acidophilus<\/jats:italic>\n                      C4 prior to FC onset significantly reduced impairment of defecation function and increased intestinal transit rate.\n                      <jats:italic toggle=\"yes\">L. acidophilus<\/jats:italic>\n                      C4 also increased the expression of tight junction proteins ZO-1 and occludin, suggesting a protective effect on the intestinal barrier. In addition,\n                      <jats:italic toggle=\"yes\">L. acidophilus<\/jats:italic>\n                      C4 was able to modulate the inflammatory response by decreasing the pro-inflammatory factor IL-1\u03b2 while increasing the anti-inflammatory cytokine IL-10. These results reveal that\n                      <jats:italic toggle=\"yes\">L. acidophilus<\/jats:italic>\n                      C4 can improve constipation through anti-inflammation and restoration of intestinal barrier function, which can be a candidate strain for the development of novel interventions for constipation.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Functional constipation (FC) requires novel therapeutic approaches. Probiotics have attracted attention in the prevention and management of gastrointestinal diseases; however, their efficacy and mechanisms of action against FC need to be verified. This study provides experimental evidence for this purpose, supporting its potential as a non-invasive and well-tolerated intervention. The core of this study is\n                        <jats:italic toggle=\"yes\">Lactobacillus acidophilus<\/jats:italic>\n                        C4, which exhibits high compatibility with the human intestinal microenvironment and lays a foundation for clinical research.\n                        <jats:italic toggle=\"yes\">L. acidophilus<\/jats:italic>\n                        C4 can enhance intestinal peristalsis, protect the intestinal barrier (by upregulating tight junction proteins ZO-1 and occludin), and regulate inflammation (by balancing the pro-inflammatory cytokine\n                        <jats:italic toggle=\"yes\">IL-1\u03b2<\/jats:italic>\n                        and anti-inflammatory cytokine\n                        <jats:italic toggle=\"yes\">IL-10<\/jats:italic>\n                        ). This strain provides a new direction for research on probiotic-based treatment of FC.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.02950-25","type":"journal-article","created":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T13:07:59Z","timestamp":1778591279000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["<i>Lactobacillus acidophilus<\/i>\n                    C4 ameliorates constipation in mice"],"prefix":"10.1128","volume":"14","author":[{"given":"Han","family":"Yan","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/017z00e58","id-type":"ROR","asserted-by":"publisher"}],"name":"The Department of Infectious Diseases, the First Affiliated Hospital of Chongqing Medical University, School of Basic Medical Sciences, Chongqing Medical University","place":["Chongqing, 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