{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T15:51:52Z","timestamp":1786117912169,"version":"build-2736575974"},"reference-count":45,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Shandong provincial natural science foundation for yougth scientists","award":["ZR2025QC243"],"award-info":[{"award-number":["ZR2025QC243"]}]},{"name":"Heze University doctoral research fund","award":["XY25BSLG12"],"award-info":[{"award-number":["XY25BSLG12"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2026,8,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Metallo-beta-lactamases (MBLs) increase the spread of drug resistance among bacteria worldwide and have severely limited the efficacy of \u03b2-lactam antibiotics. To develop alternative therapeutics, we used the MBL \u03b1-helix fragment as the original peptide, which is the main reason for carbapenem resistance, and modified it into D-amino acid to replace cyclic peptide N10. The antibacterial effect of N10 was detected through the broth microdilution method and time\u2013kill curve and growth curve detection, and the effective minimum inhibitory concentrations (MICs) of N10 to bacteria ranged from 2 to 16 \u03bcg\/mL. Based on the fluorescence microscopy, hydrogen ion SEM and TEM results, N10 showed extensive membrane damage and structural collapse. In the cell infection model, N10 decreased the levels of ROS and inflammatory factors to near-control levels at 2\u00d7 MIC.\n                      <jats:italic toggle=\"yes\">In vivo<\/jats:italic>\n                      , there was no apparent cytotoxicity or hemolytic activity even at 512 \u03bcg\/mL. The \u201c0\u20132\u201312 h\u201d therapeutic strategy also resulted in noticeable therapeutic effects in a carbapenem-resistant\n                      <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                      -infected mouse model. Our research revealed a new antimicrobial peptide source and modification strategy, which broadens the antibacterial mode and therapeutic procedure.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        In this study, we modified an metallo-beta-lactamase family protein into the novel, safe, environmentally friendly antimicrobial peptide N10, which has antimicrobial, antioxidant, and anti-inflammatory properties both\n                        <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                        and\n                        <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                        . We also investigated the preliminary antimicrobial mechanism of the biopeptide. This study broadens the use of drug resistance genes and provides direction for\n                        <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                        therapeutic strategies.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.03040-25","type":"journal-article","created":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T13:04:07Z","timestamp":1784293447000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Metallo-beta-lactamase \u03b1-helix fragment modified peptide N10 is a multifunctional antimicrobial peptide"],"prefix":"10.1128","volume":"14","author":[{"given":"Hao","family":"Lu","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/041zje040","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Pharmacy, Heze University","place":["Heze, China"]},{"id":[{"id":"https:\/\/ror.org\/023b72294","id-type":"ROR","asserted-by":"publisher"}],"name":"National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University","place":["Wuhan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaoran","family":"Zhang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/023b72294","id-type":"ROR","asserted-by":"publisher"}],"name":"National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University","place":["Wuhan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuwei","family":"Zhao","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/041zje040","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Pharmacy, Heze University","place":["Heze, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-9630-3001","authenticated-orcid":true,"given":"Wenjia","family":"Lu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/041zje040","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Pharmacy, Heze University","place":["Heze, China"]},{"id":[{"id":"https:\/\/ror.org\/023b72294","id-type":"ROR","asserted-by":"publisher"}],"name":"National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University","place":["Wuhan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fm.2025.104877"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(24)02081-6"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.lanmic.2024.07.010"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2024.172150"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.drup.2023.100954"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1039\/d4md00383g"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adp7171"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2024.e31958"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00397-20"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.5c00553"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms13030508"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.1c00138"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.synbio.2023.05.003"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms8071076"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-78658-8"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules29163944"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/med.21665"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.00308-23"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2024.107104"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.02013-21"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2024.127647"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1007084"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jare.2023.04.017"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2024.139669"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.3390\/antiox14101156"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.582779"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2022.2048972"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/cmr.00129-23"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.lana.2025.101185"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics14020141"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00115-18"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202412488"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jcim.3c01881"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.4c03073"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2025.117341"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2025.108570"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.4c05377"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics10060650"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202410893"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcis.2018.10.103"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja0780380"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00209-09"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M116.733816"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-41156-2"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics9020092"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.03040-25","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.03040-25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T14:44:16Z","timestamp":1786027456000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.03040-25"}},"subtitle":[],"editor":[{"given":"Estela Maria","family":"Galvan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,8,4]]},"references-count":45,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8,4]]}},"alternative-id":["10.1128\/spectrum.03040-25"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.03040-25","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,4]]},"assertion":[{"value":"2025-09-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-28","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e03040-25"}}