{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T12:54:07Z","timestamp":1788267247993,"version":"build-2803163510"},"reference-count":68,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T00:00:00Z","timestamp":1676332800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T00:00:00Z","timestamp":1676332800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32002310"],"award-info":[{"award-number":["32002310"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"MOST | National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFC2300800 and 2021YFC2300802"],"award-info":[{"award-number":["2021YFC2300800 and 2021YFC2300802"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"name":"The Research Funding from Lanzhou Veterinary Research Institute","award":["CAASASTIP-JBGS-20210801"],"award-info":[{"award-number":["CAASASTIP-JBGS-20210801"]}]},{"DOI":"10.13039\/501100011136","name":"The State Key Laboratory of Veterinary Etiological Biology","doi-asserted-by":"crossref","award":["SKLVEB2020YQRC01"],"award-info":[{"award-number":["SKLVEB2020YQRC01"]}],"id":[{"id":"10.13039\/501100011136","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Shanxi Provincial Agricultural and Rural Research Program","award":["2021-XXXX"],"award-info":[{"award-number":["2021-XXXX"]}]},{"name":"Fundamental Research Funds of the Chinese Academy of Agricultural Sciences","award":["1610032021017"],"award-info":[{"award-number":["1610032021017"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2023,2,14]]},"abstract":"<jats:p>\n            Dense granule proteins (GRAs) play important roles in\n            <jats:named-content content-type=\"genus-species\">Toxoplasma gondii<\/jats:named-content>\n            pathogenicity. However, the functions of many putative GRAs have not been elucidated.\n          <\/jats:p>","DOI":"10.1128\/spectrum.03078-22","type":"journal-article","created":{"date-parts":[[2022,12,14]],"date-time":"2022-12-14T14:00:49Z","timestamp":1671026449000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":17,"title":["Functional Characterization of 15 Novel Dense Granule Proteins in Toxoplasma gondii Using the CRISPR-Cas9 System"],"prefix":"10.1128","volume":"11","author":[{"given":"Xiao-Nan","family":"Zheng","sequence":"first","affiliation":[{"name":"Laboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Taigu, China"},{"name":"State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin-Lei","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China"},{"name":"Institute of Urban Agriculture, Chinese Academy of Agricultural Sciences, Chengdu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3303-930X","authenticated-orcid":true,"given":"Hany M.","family":"Elsheikha","sequence":"additional","affiliation":[{"name":"Faculty of Medicine and Health Sciences, School of Veterinary Medicine and Science, University of Nottingham, Loughborough, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China"},{"name":"Institute of Urban Agriculture, Chinese Academy of Agricultural Sciences, Chengdu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi-Wei","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Xiu","family":"Sun","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin-Cheng","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2530-4628","authenticated-orcid":true,"given":"Xing-Quan","family":"Zhu","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Taigu, China"},{"name":"Key Laboratory of Veterinary Public Health of Higher Education of Yunnan Province, College of Veterinary Medicine, Yunnan Agricultural University, Kunming, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting-Ting","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China"},{"name":"Institute of Urban Agriculture, Chinese Academy of Agricultural Sciences, Chengdu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00115-19"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S2352-3018(17)30005-X"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2019.01.005"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2017.02.007"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1007670"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M504158200"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-011720-122318"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2014.12.002"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00005-17"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2020.09.011"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00808-16"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0232552"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.8b00626"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1242\/bio.039818"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-008-7556-x"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.01.056"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201701108"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E12-11-0827"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01188-14"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1010138"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.01442-22"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2015.04.003"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/jeu.12904"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2013.03.002"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20130103"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20160340"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2016.06.006"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00674-19"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-019-0431-8"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.39887"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00603-21"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20100717"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20131272"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.13278"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12499"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201901416RR"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02388-18"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-18991-8"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00877-19"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00858-19"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00589-19"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00851-19"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00182-21"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2019.00418"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00755-16"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0903831106"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.08.019"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00487-19"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0159306"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00469-19"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003823"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.10809"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01796-18"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1816161116"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005211"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2018.00300"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2019.00321"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12749"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1008771"},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-020-06815-z"},{"key":"e_1_3_3_62_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms8030352"},{"key":"e_1_3_3_63_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-015-4791-6"},{"key":"e_1_3_3_64_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.738794"},{"key":"e_1_3_3_65_2","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2020.614759"},{"key":"e_1_3_3_66_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-021-07308-3"},{"key":"e_1_3_3_67_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0012354"},{"key":"e_1_3_3_68_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0029998"},{"key":"e_1_3_3_69_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.05182-11"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.03078-22","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.03078-22","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T14:35:51Z","timestamp":1676385351000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.03078-22"}},"subtitle":[],"editor":[{"given":"Jian","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2023,2,14]]},"references-count":68,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2023,2,14]]}},"alternative-id":["10.1128\/spectrum.03078-22"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.03078-22","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,2,14]]},"assertion":[{"value":"2022-08-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-11-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-12-14","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e03078-22"}}