{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T09:41:59Z","timestamp":1785404519637,"version":"3.56.0"},"reference-count":32,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Candida auris<\/jats:italic>\n                      , a yeast that is highly resistant to antifungal treatments, represents a significant healthcare concern due to its rapid spread through surface contamination and patient colonization. A recent outbreak at the Miguel Servet University Hospital in Zaragoza, Spain, highlighted the urgent need for rapid diagnostic responses. The current 4-day duration for microbiological culture results hinders timely patient isolation and transmission prevention. This study aims to evaluate the VIASURE\n                      <jats:italic toggle=\"yes\">Candida auris<\/jats:italic>\n                      Real-Time PCR Detection Kit (Certest Biotec) for diagnostic performance and analytical specificity compared to the standard methodology based on culture and MALDI-TOF MS characterization. From August 2023 to December 2023, the Microbiology Service of the Miguel Servet University Hospital processed 816 diagnostic samples. Accuracy testing resulted in adequate clinical validation values, as presented here: sensitivity, 0.98 (95% confidence interval [CI], 0.90 to 1); specificity, 1 (95% CI, 0.99 to 1); positive predictive value, 0.98 (95% CI, 0.90 to 1); and negative predictive value, 1 (95% CI, 0.99 to 1). The implementation of this molecular biology test in the laboratory promises to improve diagnostic efficiency by allowing rapid patient isolation, initiation of appropriate treatment, and effective isolation measures.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Candida auris<\/jats:italic>\n                      , a resilient and antifungal-resistant yeast, poses a significant healthcare threat due to its rapid spread. A recent outbreak at a hospital in Zaragoza, Spain, emphasized the urgent need for faster diagnostics. The current four-day wait for culture results hampers timely patient isolation. This retrospective and comparative study demonstrates the favorable clinical parameters of a commercially available molecular diagnostic kit. The kit allows enhanced diagnostic efficiency, swifter patient isolation, and more effective control measures in the laboratory. In conclusion, the kit addresses the pressing challenges presented by\n                      <jats:italic toggle=\"yes\">C. auris<\/jats:italic>\n                      in healthcare.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.03111-24","type":"journal-article","created":{"date-parts":[[2025,5,19]],"date-time":"2025-05-19T09:00:46Z","timestamp":1747645246000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["Enhancing\n                    <i>Candida auris<\/i>\n                    diagnosis: a comprehensive evaluation of VIASURE\n                    <i>Candida auris<\/i>\n                    real-time PCR detection kit for rapid diagnostic responses"],"prefix":"10.1128","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-7807-0400","authenticated-orcid":true,"given":"Blanca","family":"Dehesa-Garc\u00eda","sequence":"first","affiliation":[{"name":"Health Research Institute Arag\u00f3n","place":["Zaragoza, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9081-3982","authenticated-orcid":false,"given":"Henar","family":"Alonso","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/012a91z28","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology, Pediatrics, Radiology, and Public Health, Faculty of Medicine, Universidad de Zaragoza","place":["Zaragoza, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Paz","family":"Peris","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/012a91z28","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Animal Pathology, Faculty of Veterinary Sciences, Universidad de Zaragoza","place":["Zaragoza, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Blanca","family":"Fortu\u00f1o","sequence":"additional","affiliation":[{"name":"Health Research Institute Arag\u00f3n","place":["Zaragoza, Spain"]},{"name":"Miguel Servet University Hospital, Microbiology","place":["Zaragoza, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pilar","family":"Abad","sequence":"additional","affiliation":[{"name":"Health Research Institute Arag\u00f3n","place":["Zaragoza, Spain"]},{"name":"Miguel Servet University Hospital, Microbiology","place":["Zaragoza, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Rezusta","sequence":"additional","affiliation":[{"name":"Health Research Institute Arag\u00f3n","place":["Zaragoza, Spain"]},{"name":"Miguel Servet University Hospital, Microbiology","place":["Zaragoza, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Milagro","sequence":"additional","affiliation":[{"name":"Health Research Institute Arag\u00f3n","place":["Zaragoza, Spain"]},{"name":"Miguel Servet University Hospital, Microbiology","place":["Zaragoza, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-023-00861-x"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12281-021-00420-y"},{"key":"e_1_3_3_4_2","unstructured":"Centers for Disease Control and Prevention. 2016. Candida auris clinical alert to U.S. Healthcare facilities - June 2016. Available from: https:\/\/archive.cdc.gov\/www_cdc_gov\/fungal\/candida-auris\/candida-auris-alert.html. Retrieved 25 Apr 2024."},{"key":"e_1_3_3_5_2","unstructured":"Centers for Disease Control and Prevention. 2017. Candida auris clinical update - September 2017. Available from: https:\/\/archive.cdc.gov\/www_cdc_gov\/fungal\/candida-auris\/c-auris-alert-09-17.html. Retrieved 25 Apr 2024."},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1186\/s40560-018-0342-4"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(18)30609-1"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myy054"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.n1880"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.3390\/jof7020081"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-013-2027-1"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhin.2017.09.009"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/myc.12709"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13756-016-0132-5"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.15585\/mmwr.mm6619a7"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1080\/23120053.2016.1221613"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00134-014-3603-2"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01223-17"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00630-19"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/myc.13250"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.3390\/jof9080849"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.757835"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.eimc.2020.02.007"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00630-17"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01604-18"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01337-18"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.riam.2019.06.001"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.3390\/jof9090913"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.53854\/liim-3103-6"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2022.27.46.2200846"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.3390\/healthcare11030425"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2013.04.005"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2288-6-31"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.03111-24","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.03111-24","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T09:07:01Z","timestamp":1751360821000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.03111-24"}},"subtitle":[],"editor":[{"given":"Po-Yu","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,7]]},"references-count":32,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2025,7]]}},"alternative-id":["10.1128\/spectrum.03111-24"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.03111-24","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7]]},"assertion":[{"value":"2024-11-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-03","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-05-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e03111-24"}}