{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T11:19:43Z","timestamp":1785410383715,"version":"3.56.0"},"reference-count":20,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T00:00:00Z","timestamp":1762214400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T00:00:00Z","timestamp":1762214400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,11,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      This study characterized compositional shifts in cervical microbiota across disease stages from benign conditions through cervical intraepithelial neoplasia (CIN) to cervical cancer (CC) and investigated interactions with high-risk HPV (hr-HPV) infection using species-resolution profiling to identify severity-associated biomarkers. Cervical exfoliated epithelial cells from 50 patients (eight normal\/CIN1, 15 CIN2, 19 CIN3, 5 CC) were analyzed using Low-Coverage Whole Genome Sequencing combined with the Ultrasensitive Chromosomal Aneuploidy Detector (UCAD), a technology featuring a two-step normalization framework that systematically converts raw microbial reads into statistically validated abundance deviations. This enables quantitative identification of pathologically relevant microbiota through cohort-wide Z-score benchmarking. Microbial diversity, differential biomarkers, and HPV-microbiota interactions were assessed using Kruskal-Wallis tests, LEfSe, and Random Forest modeling. Results revealed progressive\n                      <jats:italic toggle=\"yes\">Lactobacillus<\/jats:italic>\n                      depletion (e.g.,\n                      <jats:italic toggle=\"yes\">Lactobacillus crispatus<\/jats:italic>\n                      : 32.9% in \u2264CIN2 vs. 8.8% in CC) and enrichment of pathobionts like\n                      <jats:italic toggle=\"yes\">Gardnerella<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">Bacteroides<\/jats:italic>\n                      with lesion severity. CC exhibited the highest microbial diversity (Shannon index: CC vs. CIN2,\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      =0.045), dominated by HPV16 (11.8%),\n                      <jats:italic toggle=\"yes\">Bacteroides<\/jats:italic>\n                      (55.4%), and\n                      <jats:italic toggle=\"yes\">Porphyromonas<\/jats:italic>\n                      (25.2%). LEfSe identified HPV16, HPV35,\n                      <jats:italic toggle=\"yes\">Parvimonas micra<\/jats:italic>\n                      , and\n                      <jats:italic toggle=\"yes\">Anaerococcus lactolyticus<\/jats:italic>\n                      as CC-specific markers, while Random Forest highlighted\n                      <jats:italic toggle=\"yes\">Mobiluncus curtisii<\/jats:italic>\n                      (importance score=2.0) and HPV16 as key discriminators. CC microbiota showed significant Bacteroidetes enrichment (82% at class level) and reduced Firmicutes abundance. These findings suggest carcinogenesis-associated microbial restructuring, marked by\n                      <jats:italic toggle=\"yes\">Lactobacillus<\/jats:italic>\n                      loss, anaerobic proliferation, and HPV16\/35 dominance, potentially modulating disease progression. The identified signatures may inform diagnostic development and microbiome-targeted therapies.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>Our study pioneers an LC-WGS\/UCAD approach to characterize microbial across the spectrum from benign lesions through precancerous cervical intraepithelial neoplasia to invasive cervical carcinoma. By identifying lesion-specific microbial biomarkers and HPV-associated cofactors, this work advances mechanistic understanding of microbiota-driven oncogenesis and informs future strategies for microbiota-targeted cervical cancer prevention.<\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.03206-24","type":"journal-article","created":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T10:48:31Z","timestamp":1759920511000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Characterization of cervical microbiota in cervical intraepithelial neoplasia and cervical cancer using low-coverage whole genome sequencing"],"prefix":"10.1128","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-0103-0889","authenticated-orcid":false,"given":"Tingting","family":"Zhang","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01eff5662","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Obstetrics and Gynecology, Beijing Chaoyang Hospital Affiliated with the Capital Medical University","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Gu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01eff5662","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Obstetrics and Gynecology, Beijing Chaoyang Hospital Affiliated with the Capital Medical University","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weihua","family":"Li","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01eff5662","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Obstetrics and Gynecology, Beijing Chaoyang Hospital Affiliated with the Capital Medical University","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruxue","family":"Han","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01eff5662","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Obstetrics and Gynecology, Beijing Chaoyang Hospital Affiliated with the Capital Medical University","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyu","family":"Liu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01eff5662","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Obstetrics and Gynecology, Beijing Chaoyang Hospital Affiliated with the Capital Medical University","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chan","family":"Dai","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00xn0ew58","id-type":"ROR","asserted-by":"publisher"}],"name":"Suzhou Hongyuan Biotech Inc., Biobay","place":["Suzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Di","family":"Zhang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01eff5662","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Obstetrics and Gynecology, Beijing Chaoyang Hospital Affiliated with the Capital Medical University","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1172-6750","authenticated-orcid":true,"given":"Hua","family":"Li","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01eff5662","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Obstetrics and Gynecology, Beijing Chaoyang Hospital Affiliated with the Capital Medical University","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21660"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(13)60022-7"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.3390\/biology11081114"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S2666-5247(23)00315-4"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics11111996"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-016-0203-0"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrg.2016.49"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2007.08.006"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-20-0401"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(10)70230-8"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.14218\/jctp.2023.00004"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijgo.2015.07.038"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/cam4.4801"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-021-02152-y"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-33750-y"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-09842-6"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.02533"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41585-020-0286-z"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.gpb.2021.08.001"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkae978"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.03206-24","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.03206-24","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T14:10:28Z","timestamp":1762265428000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.03206-24"}},"subtitle":[],"editor":[{"given":"Hyun Jin","family":"Kwun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,11,4]]},"references-count":20,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2025,11,4]]}},"alternative-id":["10.1128\/spectrum.03206-24"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.03206-24","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,4]]},"assertion":[{"value":"2024-12-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-02","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e03206-24"}}