{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T15:21:09Z","timestamp":1788448869997,"version":"build-2803163510"},"reference-count":27,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T00:00:00Z","timestamp":1783382400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T00:00:00Z","timestamp":1783382400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Medical Science and Technology Project of Henan Province","award":["LHGJ2024020581"],"award-info":[{"award-number":["LHGJ2024020581"]}]},{"name":"Key Reseatch, Development, and Promotion Projects of Henan Province","award":["242102310328"],"award-info":[{"award-number":["242102310328"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2026,7,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      The rise of carbapenem-resistant\n                      <jats:italic toggle=\"yes\">Enterobacterales<\/jats:italic>\n                      presents a substantial global public health challenge. While carbapenem-resistant\n                      <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                      is rarely reported in clinical settings, this study characterizes a carbapenem resistance mediated by\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>NDM-13<\/jats:sub>\n                      <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                      Stanley strain SAL22057, isolated from the fecal sample of a pediatric patient with diarrhea and fever. We determined antimicrobial susceptibility, conducted genomic characterization, and assessed plasmid conjugation. Antimicrobial susceptibility testing showed that SAL22057 was resistant to meropenem (minimum inhibitory concentration [MIC] 32 \u03bcg\/mL) and imipenem (MIC 16 \u03bcg\/mL). Genotyping analysis identified SAL22057 as belonging to ST29, and it was found to be multidrug-resistant and to carry numerous virulence genes. Whole-genome sequencing and plasmid analysis identified that\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>NDM-13<\/jats:sub>\n                      was located on pSAL22057-NDM (IncI1\n                      <jats:italic toggle=\"yes\">\u03b1<\/jats:italic>\n                      ), while a distinct plasmid, pSAL22057-OXA (IncHI2), harbored multiple antimicrobial resistance genes, including\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>OXA-10<\/jats:sub>\n                      . Conjugation experiments revealed that\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>NDM-13<\/jats:sub>\n                      was transferable to\n                      <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                      C600. We show that IS\n                      <jats:italic toggle=\"yes\">1294<\/jats:italic>\n                      likely mediates\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>NDM-13<\/jats:sub>\n                      mobilization, with its insertion influencing gene transfer. Furthermore, the consistent flanking of several resistance genes by IS\n                      <jats:italic toggle=\"yes\">26<\/jats:italic>\n                      elements indicates that IS\n                      <jats:italic toggle=\"yes\">26<\/jats:italic>\n                      -mediated horizontal gene transfer is a key mechanism driving the dissemination of these determinants and genomic rearrangements within\n                      <jats:italic toggle=\"yes\">Enterobacteriaceae<\/jats:italic>\n                      .\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        The emergence of carbapenem-resistant\n                        <jats:italic toggle=\"yes\">Enterobacterales<\/jats:italic>\n                        poses a global health threat. This study identified a carbapenem-resistant\n                        <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                        Stanley strain, SAL22057, from a pediatric patient, which carried the carbapenemase gene\n                        <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                        <jats:sub>NDM-13<\/jats:sub>\n                        . The genotyping revealed that SAL22057 is ST29, displaying a concerning multidrug-resistant phenotype along with several virulence determinants. Alarmingly, this strain exhibits high-level resistance to meropenem (minimum inhibitory concentration [MIC] 32 \u03bcg\/mL) and imipenem (MIC 16 \u03bcg\/mL). Conjugation experiments confirm that\n                        <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                        <jats:sub>NDM-13<\/jats:sub>\n                        is transferable to\n                        <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                        C600, signaling a clear pathway for resistance dissemination among\n                        <jats:italic toggle=\"yes\">Enterobacteriaceae<\/jats:italic>\n                        . We demonstrate that IS\n                        <jats:italic toggle=\"yes\">1294<\/jats:italic>\n                        likely mobilizes\n                        <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                        <jats:sub>NDM-13<\/jats:sub>\n                        , while IS\n                        <jats:italic toggle=\"yes\">26<\/jats:italic>\n                        consistently flanks multiple resistance genes, providing mechanistic evidence that IS\n                        <jats:italic toggle=\"yes\">26<\/jats:italic>\n                        -mediated transposition is accelerating the spread of resistance genes in clinical pathogens.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.03207-24","type":"journal-article","created":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T13:01:05Z","timestamp":1781010065000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["Carbapenem resistance mediated by\n                    <i>bla<\/i>\n                    <sub>NDM-13<\/sub>\n                    in a highly drug-resistant\n                    <i>Salmonella<\/i>\n                    Stanley ST29 strain in China"],"prefix":"10.1128","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4066-455X","authenticated-orcid":true,"given":"Juanjuan","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory, Children\u2019s Hospital Affiliated to Zhengzhou University, Henan Children\u2019s Hospital, Zhengzhou Children\u2019s Hospital, Zhengzhou Key Laboratory of Children\u2019s Infection and 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