{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T14:19:33Z","timestamp":1790691573513,"version":"4.1.0"},"reference-count":24,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2024,4,2]],"date-time":"2024-04-02T00:00:00Z","timestamp":1712016000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,4,2]],"date-time":"2024-04-02T00:00:00Z","timestamp":1712016000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"UB | Institute of Veterinary Bacteriology, University of Bern","award":["660-50"],"award-info":[{"award-number":["660-50"]}]},{"name":"Clinic for Ruminants, University of Bern","award":["33-014"],"award-info":[{"award-number":["33-014"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2024,4,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              The fattening of calves is often associated with high antimicrobial use and the selection of antimicrobial resistance (AMR). The objective of this observational longitudinal study was to describe the AMR and strain dynamics, using whole-genome sequencing (WGS), of fecal\n              <jats:italic>Escherichia coli<\/jats:italic>\n              in a cohort of 22 calves. All calves received antimicrobial group treatments on Day (D) 1 (oxytetracycline, intramuscularly) and on D4 through D12 (doxycycline, in-feed). Additionally, eight calves received individual parenteral treatments between D7 and D59, including florfenicol, amoxicillin, marbofloxacin, and gamithromycin. Rectal swabs were collected from all calves on D1 (prior to treatment), D2, D9, and D82. The swabs were spread onto\n              <jats:italic>Enterobacterales<\/jats:italic>\n              -selective agar, and three\n              <jats:italic>E. coli<\/jats:italic>\n              colonies per plate were subjected to WGS. Out of 264 isolates across all calves and sampling times, 80 unique strains were identified, a majority of which harbored genes conferring resistance to tetracyclines, streptomycin, and sulfonamides. The diversity of strains decreased during the in-feed antimicrobial group treatment of the calves. On D82, 90% of isolates were strains that were not isolated at previous sampling times, and the median number per strain of AMR determinants to tetracyclines, florfenicol, \u03b2-lactams, quinolones, or macrolides decreased compared to D9. Additionally, clonal dissemination of some strains represented the main transmission route of AMR determinants. In this study, WGS revealed important variations in strain diversity and genotypic AMR of fecal\n              <jats:italic>E. coli<\/jats:italic>\n              over time in calves subjected to group antimicrobial treatments.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              The continued emergence and spread of antimicrobial resistance (AMR) determinants are serious global concerns. The dynamics of AMR spread and persistence in bacterial and animal host populations are complex and not solely driven by antimicrobial selection pressure. In calf fattening, both antimicrobial use and carriage prevalence of antimicrobial-resistant bacteria are generally recognized as high. This study provides new insights into the short-term, within-farm dynamics and transmission of AMR determinants in\n              <jats:italic>Escherichia coli<\/jats:italic>\n              from the dominant fecal flora of calves subjected to antimicrobial group treatments during the rearing period. The diversity of\n              <jats:italic>E. coli<\/jats:italic>\n              strains decreased over time, although, in contrast to previous observations in extended-spectrum \u03b2-lactamase-producing\n              <jats:italic>Enterobacterales<\/jats:italic>\n              , the predominance of a few clones was not observed. The spread of AMR determinants occurred through the dissemination of clonal strains among calves. The median number per strain of AMR determinants conferring resistance to selected antimicrobials decreased toward the end of the rearing period.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.03214-23","type":"journal-article","created":{"date-parts":[[2024,3,5]],"date-time":"2024-03-05T15:02:35Z","timestamp":1709650955000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":9,"title":["Whole-genome sequencing-based antimicrobial resistance and shedding dynamics of\n            <i>Escherichia coli<\/i>\n            isolated from calves before and after antimicrobial group treatments"],"prefix":"10.1128","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0448-5386","authenticated-orcid":true,"given":"V\u00e9ronique","family":"Bernier Gosselin","sequence":"first","affiliation":[{"name":"Clinic for Ruminants, Vetsuisse Faculty, University of Bern, Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier E.","family":"Fernandez","sequence":"additional","affiliation":[{"name":"Division of Molecular Bacterial Epidemiology and Infectious Diseases, Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Ollagnier","sequence":"additional","affiliation":[{"name":"Pigs, Agroscope, Posieux, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Morel","sequence":"additional","affiliation":[{"name":"Ruminant Nutrition and Emissions, Agroscope, Posieux, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raphael","family":"Siegenthaler","sequence":"additional","affiliation":[{"name":"Research Contracts Animals, Agroscope, Posieux, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Collaud","sequence":"additional","affiliation":[{"name":"Division of Molecular Bacterial Epidemiology and Infectious Diseases, Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mireille","family":"Meylan","sequence":"additional","affiliation":[{"name":"Clinic for Ruminants, Vetsuisse Faculty, University of Bern, Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5722-9445","authenticated-orcid":true,"given":"Vincent","family":"Perreten","sequence":"additional","affiliation":[{"name":"Division of Molecular Bacterial Epidemiology and Infectious Diseases, Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2016.02.002"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2016.01.020"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0146488"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkr570"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2019.03.007"},{"key":"e_1_3_5_7_2","unstructured":"BLV (Bundesamt f\u00fcr Lebensmittelsicherheit und Veterin\u00e4rwesen). 2022. 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