{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T15:51:59Z","timestamp":1786117919783,"version":"build-2736575974"},"reference-count":58,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2026,8,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Obesity and excess body weight in companion animals represent significant and growing health concerns. Accumulating evidence indicates that obesity-associated metabolic and inflammatory dysfunction may be mitigated through dietary modulation of host-microbiome interactions. In this controlled dietary intervention study, healthy Beagle dogs were fed a commercially available high-caloric, protein-, and lipid-enriched diet at three times their daily energy requirement for 8 weeks and orally supplemented with either pasteurized\n                      <jats:italic toggle=\"yes\">Akkermansia muciniphila<\/jats:italic>\n                      Muc\n                      <jats:sup>T<\/jats:sup>\n                      or a placebo. Supplementation reduced weight gain and attenuated the high-caloric diet-induced expansion of\n                      <jats:italic toggle=\"yes\">Peptacetobacter hiranonis<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">Collinsella<\/jats:italic>\n                      spp. compared to controls. Pasteurized\n                      <jats:italic toggle=\"yes\">A. muciniphila<\/jats:italic>\n                      -treated animals exhibited reduced circulating pro-inflammatory cytokines and a marked decrease in fecal calprotectin levels, indicative of reduced mucosal inflammation. Longitudinal modeling identified treatment-associated alterations in inflammatory markers, while integrative multi-omics factor analysis (MOFA) and redundancy analysis (RDA) revealed coordinated changes across inflammatory, metabolic, and bile acid profiles that distinguished placebo- and pasteurized\n                      <jats:italic toggle=\"yes\">A. muciniphila<\/jats:italic>\n                      -treated animals under high-caloric feeding conditions. These changes were accompanied by normalization of circulating bile acid profiles, particularly reductions in microbiome-derived secondary bile acids and oxo-derivatives associated with metabolic dysfunction. Together, these findings provide integrative insight into the immune-metabolic effects of pasteurized\n                      <jats:italic toggle=\"yes\">A. muciniphila<\/jats:italic>\n                      Muc\n                      <jats:sup>T<\/jats:sup>\n                      in dogs under high-caloric feeding conditions and support its potential as a dietary intervention to mitigate obesity-associated dysfunction in companion animals. As this is an exploratory proof-of-concept study conducted in a limited number of animals under controlled feeding conditions, further validation in larger and more diverse companion animal populations is warranted.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Obesity in pet dogs is increasingly recognized not only as a condition of excess body fat but as a chronic inflammatory disorder with profound metabolic consequences. This study demonstrates that dietary supplementation with pasteurized\n                        <jats:italic toggle=\"yes\">Akkermansia muciniphila<\/jats:italic>\n                        Muc\n                        <jats:sup>T<\/jats:sup>\n                        can counteract the adverse effects of a high-caloric diet in dogs by reducing body weight gain, preventing inflammation, and restoring bile acid homeostasis. These results highlight the therapeutic potential of gut-targeted nutritional strategies for managing obesity in companion animals and introduce\n                        <jats:italic toggle=\"yes\">A. muciniphila<\/jats:italic>\n                        Muc\n                        <jats:sup>T<\/jats:sup>\n                        as a safe, effective postbiotic with translational relevance for pet health.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.03226-25","type":"journal-article","created":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T13:02:46Z","timestamp":1784034166000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Pasteurized\n                    <i>Akkermansia muciniphila<\/i>\n                    Muc\n                    <sup>T<\/sup>\n                    reduces high-caloric diet-induced weight gain and alters bile acid and inflammatory profiles in dogs"],"prefix":"10.1128","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1526-1516","authenticated-orcid":false,"given":"Harro M.","family":"Timmerman","sequence":"first","affiliation":[{"name":"Microbiome Innovation Consultancy","place":["Rhenen, the Netherlands"]},{"name":"The Akkermansia Company","place":["Mont-Saint-Guibert, Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6714-1209","authenticated-orcid":true,"given":"Anneleen","family":"Segers","sequence":"additional","affiliation":[{"name":"The Akkermansia Company","place":["Mont-Saint-Guibert, Belgium"]},{"name":"Laboratory of Microbiology, Wageningen University","place":["Wageningen, the Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos","family":"Boekhorst","sequence":"additional","affiliation":[{"name":"Laboratory of Host-Microbiome Interactions, Wageningen University","place":["Wageningen, the Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dennis","family":"te Beest","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04qw24q55","id-type":"ROR","asserted-by":"publisher"}],"name":"Biometris, Wageningen University & Research","place":["Wageningen, the Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chi-Hsuan","family":"Sung","sequence":"additional","affiliation":[{"name":"Gastrointestinal Laboratory, Texas A&M College of Veterinary Medicine & Biomedical Sciences","place":["College Station, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda B.","family":"Blake","sequence":"additional","affiliation":[{"name":"Gastrointestinal Laboratory, Texas A&M College of Veterinary Medicine & Biomedical Sciences","place":["College Station, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan 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