{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:34:06Z","timestamp":1787016846599,"version":"build-2736575974"},"reference-count":31,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T00:00:00Z","timestamp":1756771200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T00:00:00Z","timestamp":1756771200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"crossref","award":["2024JJ5217"],"award-info":[{"award-number":["2024JJ5217"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004735","name":"Hunan Provincial Natural Science Foundation of China","doi-asserted-by":"crossref","award":["2023JJ30324"],"award-info":[{"award-number":["2023JJ30324"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Hunan Provincial Health High-level Talent Scientific research project","award":["R2023156"],"award-info":[{"award-number":["R2023156"]}]},{"name":"Ministry of Science and Technology in Hunan Provincial of China","award":["2018TP1028"],"award-info":[{"award-number":["2018TP1028"]}]},{"name":"Hunan Provincial Health High-level Talent Scientific research project","award":["R2023143"],"award-info":[{"award-number":["R2023143"]}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFC2701800,2021YFC2701805"],"award-info":[{"award-number":["2021YFC2701800,2021YFC2701805"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Shanghai Municiple Science and Technology Major Project","award":["ZD2021CY001"],"award-info":[{"award-number":["ZD2021CY001"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,9,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Invasive candidiasis (IC) is the most common fungal infection in clinical settings, yet early diagnosis remains challenging. Candidalysin, a key virulence factor in invasive infections, exhibits site-specific pathogenicity. Our study identifies candidalysin as a serodominant antigenic peptide and reveals significantly elevated serum anti-candidalysin IgG levels in IC across diverse anatomical sites, suggesting its potential as a novel diagnostic biomarker. To evaluate this, we developed an indirect enzyme-linked immunosorbent assay (ELISA) to quantify serum anti-candidalysin IgG. The assay was validated in two distinct validation subsets using a 5-year cohort comprising 121 proven, probable, or possible IC cases from Hunan Children\u2019s Hospital, alongside 105 non-IC controls (patients with other infections and healthy individuals). In validation set 1, comparing proven IC cases (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 20) to non-IC controls, the cutoff increased to 0.1768 ng\/mL, with an area under the curve (AUC) of 0.818 (95% CI: 0.736\u20130.899), 80% sensitivity, and 73.3% specificity. In validation set 2, receiver operating characteristic (ROC) analysis comparing proven\/probable IC cases (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 77) to non-IC controls (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 105) yielded an AUC of 0.870 (95% CI: 0.821\u20130.919) at an optimal cutoff of 0.1647 ng\/mL, demonstrating 87% sensitivity and 70.5% specificity. These findings establish the indirect ELISA for anti-candidalysin IgG as a robust diagnostic tool for IC. Additionally, the assay effectively discriminates colonization from invasive infection at non-sterile sites in suspected cases, offering valuable guidance for antifungal therapy and prophylaxis.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>The indirect enzyme-linked immunosorbent assay (ELISA) for detecting serum anti-candidalysin IgG exhibits robust diagnostic performance in invasive candidiasis, demonstrating particular utility in distinguishing commensal colonization from invasive infection in cases of suspected deep-seated candidiasis. This assay represents a valuable adjunctive tool for both clinical diagnosis and therapeutic management of invasive candidiasis.<\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.03245-24","type":"journal-article","created":{"date-parts":[[2025,8,5]],"date-time":"2025-08-05T14:01:31Z","timestamp":1754402491000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["Detection of serum anti-candidalysin IgG by indirect ELISA: a novel auxiliary tool for diagnosing invasive candidiasis in a preliminary pediatric study"],"prefix":"10.1128","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-7295-4494","authenticated-orcid":false,"given":"Ting","family":"Luo","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00f1zfq44","id-type":"ROR","asserted-by":"publisher"}],"name":"Pediatrics Research Institute of the Affiliated Children\u2019s 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