{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T07:59:24Z","timestamp":1787990364020,"version":"build-2784847793"},"reference-count":46,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T00:00:00Z","timestamp":1759795200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T00:00:00Z","timestamp":1759795200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Central Government-Directed Project for Local Science and Technology Development","award":["2024ZYD0144 and 24ZYZYTS0389"],"award-info":[{"award-number":["2024ZYD0144 and 24ZYZYTS0389"]}]},{"DOI":"10.13039\/501100020207","name":"Health Commission of Sichuan Province","doi-asserted-by":"publisher","award":["24WXXT05"],"award-info":[{"award-number":["24WXXT05"]}],"id":[{"id":"10.13039\/501100020207","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,10,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Dysbiosis of the gastric microecology is implicated in various gastric diseases, with\n                      <jats:italic toggle=\"yes\">Helicobacter pylori<\/jats:italic>\n                      (\n                      <jats:italic toggle=\"yes\">H. pylori<\/jats:italic>\n                      ) infection serving as a pivotal factor influencing the gastric microecological balance and vice versa. In this study, we investigated the novel effects of gastric microbiota transplantation (GMT) on gastric microecology and the potential of this treatment to enhance\n                      <jats:italic toggle=\"yes\">H. pylori<\/jats:italic>\n                      eradication. We performed a metagenomic analysis of the microecological systems across different regions of the stomach, including the gastric fluid (GF), the gastric mucus layer (GML), and the gastric mucosa (GM). We initiated a clinical GMT intervention by transplanting microbial communities from healthy individuals' GML into patients exhibiting refractory\n                      <jats:italic toggle=\"yes\">H. pylori<\/jats:italic>\n                      infection and chronic atrophic gastritis. Our findings demonstrated significant disparities in species richness among the GF, GML, and GM, with the GML exhibiting the highest diversity of unique microbial genera.\n                      <jats:italic toggle=\"yes\">H. pylori<\/jats:italic>\n                      infection primarily influenced the relative species abundance within the GML community, without altering its fundamental composition. Clinically, GMT was well-tolerated by all recipients and showed substantial synergistic efficacy against refractory\n                      <jats:italic toggle=\"yes\">H. pylori<\/jats:italic>\n                      infection, achieving a 100% eradication rate in all patients, and significantly alleviating symptoms in individuals with\n                      <jats:italic toggle=\"yes\">H. pylori<\/jats:italic>\n                      -positive atrophic gastritis (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      &lt; 0.05). Compared with the gastric microbiota of\n                      <jats:italic toggle=\"yes\">H. pylori<\/jats:italic>\n                      -negative patients, the gastric microbiota of\n                      <jats:italic toggle=\"yes\">H. pylori<\/jats:italic>\n                      -positive patients treated with GMT exhibited closer alignment with those of healthy donors. In conclusion, GMT utilizing GML enhanced the eradication rate of refractory\n                      <jats:italic toggle=\"yes\">H. pylori<\/jats:italic>\n                      infection and improved symptoms in patients with\n                      <jats:italic toggle=\"yes\">H. pylori<\/jats:italic>\n                      -positive atrophic gastritis through modulating the gastric microbiota.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      Dysbiosis of the gastric microecology is implicated in various gastric diseases, with\n                      <jats:italic toggle=\"yes\">Helicobacter pylori (H. pylori)<\/jats:italic>\n                      infection serving as a pivotal factor influencing the gastric microecological balance and vice versa. We investigated the novel effects of gastric microbiota transplantation (GMT) on gastric microecology and the potential of this treatment to enhance\n                      <jats:italic toggle=\"yes\">H. pylori<\/jats:italic>\n                      eradication. GMT significantly enhanced the eradication rate of refractory\n                      <jats:italic toggle=\"yes\">H. pylori<\/jats:italic>\n                      infection and improved symptoms in patients with\n                      <jats:italic toggle=\"yes\">H. pylori<\/jats:italic>\n                      -positive atrophic gastritis. GMT demonstrated improvements in the cure rate of refractory\n                      <jats:italic toggle=\"yes\">H. pylori<\/jats:italic>\n                      infection, potentially offering a new clinical treatment approach. This finding provides new insights and a potential therapeutic direction for treating dysbiosis related chronic gastric diseases.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.03263-24","type":"journal-article","created":{"date-parts":[[2025,8,18]],"date-time":"2025-08-18T13:08:19Z","timestamp":1755522499000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["Gastric microbiota transplantation enhanced the eradication of refractory\n                    <i>Helicobacter pylori<\/i>\n                    infection by modulating the gastric microbiota: a pilot study"],"prefix":"10.1128","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9880-1356","authenticated-orcid":false,"given":"Jun","family":"Li","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01c4jmp52","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of 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