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in pre-clinical studies; however, other clinical phage therapy studies have reported the occurrence of phage-resistant variants. Here, we assessed phage resistance using a cytolytic\n              <jats:italic>E. faecalis<\/jats:italic>\n              clinical isolate, EF01. After infecting EF01 with \u03a6Ef2.1 (\n              <jats:italic>Myoviridae<\/jats:italic>\n              ) or \u03a6Ef2.2 (\n              <jats:italic>Podoviridae<\/jats:italic>\n              ), four host variants (R-EF01\n              <jats:sup>\u03a6Ef2.1<\/jats:sup>\n              -A and R-EF01\n              <jats:sup>\u03a6Ef2.1<\/jats:sup>\n              -B from infection with \u03a6Ef2.1, and R-EF01\n              <jats:sup>\u03a6Ef2.2<\/jats:sup>\n              -A and R-EF01\n              <jats:sup>\u03a6Ef2.2<\/jats:sup>\n              -B from infection with \u03a6Ef2.2) were isolated. Although isolate R-EF01\n              <jats:sup>\u03a6Ef2.2<\/jats:sup>\n              exhibited resistance to both phages, isolate R-EF01\n              <jats:sup>\u03a6Ef2.1<\/jats:sup>\n              demonstrated partial resistance only to \u03a6Ef2.1. Whole-genome sequencing of these four isolates revealed an insertion sequence, IS256, -mediated disruption of\n              <jats:italic>xylA<\/jats:italic>\n              in R-EF01\n              <jats:sup>\u03a6Ef2.1<\/jats:sup>\n              -A and R-EF01\n              <jats:sup>\u03a6Ef2.1<\/jats:sup>\n              -B. In addition, a non-synonymous mutation in\n              <jats:italic>epaR<\/jats:italic>\n              , essential for the complete\n              <jats:italic>Enterococcus<\/jats:italic>\n              polysaccharide antigen (Epa), was identified in the R-EF01\n              <jats:sup>\u03a6Ef2.2<\/jats:sup>\n              -A isolate. Furthermore, R-EF01\n              <jats:sup>\u03a6Ef2.2<\/jats:sup>\n              isolates exhibited IS256-associated chromosomal deletions and lacked\n              <jats:italic>galE<\/jats:italic>\n              , a gene involved in Epa biosynthesis. After gavaging mice with EF01 WT, R-EF01\n              <jats:sup>\u03a6Ef2.1<\/jats:sup>\n              -A, R-EF01\n              <jats:sup>\u03a6Ef2.2<\/jats:sup>\n              -A, and R-EF01\n              <jats:sup>\u03a6Ef2.2<\/jats:sup>\n              -B isolates, colonization of R-EF01\n              <jats:sup>\u03a6Ef2.2<\/jats:sup>\n              isolates was significantly attenuated. R-EF01\n              <jats:sup>\u03a6Ef2.2<\/jats:sup>\n              isolates exhibited less resistance to the bile salt sodium deoxycholate and showed reduced adherence to intestinal cell monolayers, suggesting that phage-resistant variants with alterations in bacterial surface molecules, potentially including those involved in Epa biosynthesis, reduced pathogen fitness by attenuating gut colonization. In summary, IS256 is involved in phage resistance of a cytolytic\n              <jats:italic>E. faecalis<\/jats:italic>\n              clinical isolate, and certain phage resistance mechanisms could contribute to favorable clinical outcomes by promoting the swift elimination of phage-resistant variants in the treatment of alcohol-associated hepatitis.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              Phage therapy is a promising approach for precise editing of the gut microbiota. Notably, the specific elimination of cytolytic\n              <jats:italic>E. faecalis<\/jats:italic>\n              from the intestinal microbiota by phages attenuates ethanol-induced liver disease in pre-clinical studies. Despite the great promise of phage therapy, the occurrence of phage-resistant variants represents a concern for the successful development of phage-based therapies. In this context, we assessed phage resistance using a cytolytic\n              <jats:italic>E. faecalis<\/jats:italic>\n              clinical isolate. Isolated phage-resistant variants acquired mutations or deletions of Epa biosynthesis-related genes and exhibited attenuated colonization in the gut. These phage-resistant variants showed less resistance to bile salts and reduced adherence to intestinal cell monolayers. These results suggest that even if phage-resistant variants arise during phage therapy, certain mechanisms of phage resistance may contribute to the rapid elimination of phage-resistant variants promoting favorable clinical outcomes in the treatment of alcohol-associated hepatitis.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.03303-24","type":"journal-article","created":{"date-parts":[[2025,4,15]],"date-time":"2025-04-15T13:00:21Z","timestamp":1744722021000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["Insertion sequence-mediated phage resistance contributes to attenuated colonization of cytolytic\n            <i>Enterococcus faecalis<\/i>\n            variants in the gut"],"prefix":"10.1128","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0476-752X","authenticated-orcid":true,"given":"Jumpei","family":"Fujiki","sequence":"first","affiliation":[{"name":"Department of Medicine, University of California San Diego","place":["La Jolla, USA"]},{"name":"Department of Veterinary Medicine, Rakuno Gakuen University","place":["Ebetsu, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomohiro","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California San Diego","place":["La Jolla, USA"]},{"name":"Department of Veterinary Medicine, Rakuno Gakuen University","place":["Ebetsu, Japan"]},{"name":"Laboratory of Small Animal Surgery, School of Veterinary Medicine, Azabu University","place":["Kanagawa, Japan"]},{"name":"Research Center for Drug and Vaccine Development, National Institute of Infectious Diseases","place":["Shinjuku, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henriette","family":"Kreimeyer","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California San Diego","place":["La Jolla, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Llorente","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California San Diego","place":["La Jolla, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4323-7668","authenticated-orcid":true,"given":"Derrick E.","family":"Fouts","sequence":"additional","affiliation":[{"name":"Department of Human Genomic Medicine, J. 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