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This study investigated the effects of CGA on the intestinal microbiota and metabolites of broilers under immune stress. A group of 312 broiler chicks was randomly divided into four treatment groups: Saline control, lipopolysaccharide (LPS), LPS + CGA (LCGA), and Saline + CGA (SCGA). The SCGA and LCGA groups were fed a basal diet supplemented with 1,000 mg\/kg CGA throughout the whole experimental period. The LPS and LCGA groups were injected with 0.5 mg\/kg LPS on days 14, 15, and 16 to induce immune stress. At day 17, the ileal contents were collected for analysis. Results showed that LPS-induced immune stress decreased the abundance of\n                      <jats:italic toggle=\"yes\">Subdoligranulum<\/jats:italic>\n                      , thereby lowering microbial richness. However, dietary CGA increased beneficial bacteria like\n                      <jats:italic toggle=\"yes\">Clostridiaceae<\/jats:italic>\n                      (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      &lt; 0.05) and\n                      <jats:italic toggle=\"yes\">Candidatus Arthromitus<\/jats:italic>\n                      (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      &lt; 0.01) in immune-stressed broilers, while reducing harmful\n                      <jats:italic toggle=\"yes\">Lachnospiraceae<\/jats:italic>\n                      (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      &lt; 0.05) and\n                      <jats:italic toggle=\"yes\">Desulfovibrionaceae<\/jats:italic>\n                      (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      &lt; 0.05). Non-targeted metabolomics revealed that immune stress significantly increased methylmalonic acid, N-acetyl-L-aspartic acid, trans-ferulic acid, and pantothenic acid, while significantly downregulating pyroglutamic acid, L-glutamic gamma-semialdehyde, and biliverdin, indicating that immune stress (LPS) induced inflammation and oxidative damage. CGA addition reduced corticosterone, prostaglandin B2, trans-ferulic acid, and methylmalonic acid levels, while increasing pyroglutamic acid, L-glutamic gamma-semialdehyde, biliverdin, chenodeoxycholic acid, desmosterol, and 4-hydroxycinnamic acid, confirming that CGA can alleviate inflammation and enhance antioxidant capacity by influencing ileal microbiota and specific metabolite synthesis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>Our previous research indicated that CGA could effectively alleviate immune stress in broilers. However, it was unclear whether its antistress effects were achieved by altering the gut microbiota and metabolites in the ileum of immune-stressed broilers. In this study, CGA altered gut microbiota and metabolites associated with alleviating immune stress were provided, which will provide new insights into strategies to target gut microbiota and metabolites to relieve immune stress in broilers. These findings contribute to a better understanding of the mechanism of action of CGA on the attenuation of immune stress and provide new approaches to immune stress therapy by regulating gut microbiota and metabolites.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.03312-24","type":"journal-article","created":{"date-parts":[[2025,6,12]],"date-time":"2025-06-12T09:00:52Z","timestamp":1749718852000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["Chlorogenic acid alters ileal microbiota and metabolites in broiler chickens under immune stress"],"prefix":"10.1128","volume":"13","author":[{"given":"Ziwei","family":"Wang","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05d80kz58","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Animal Physiology, College of Animal Science and Technology, Henan University of Science and Technology","place":["Luoyang, 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