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While\n              <jats:italic>B. pseudomallei<\/jats:italic>\n              is the causative agent of melioidosis in humans and animals, members of the\n              <jats:italic>B. cepacia<\/jats:italic>\n              complex typically only cause disease in immunocompromised hosts. In this study, we report the identification of\n              <jats:italic>B. cepacia<\/jats:italic>\n              strains isolated from either patients or soil in Laos and Thailand that express a\n              <jats:italic>B. pseudomallei<\/jats:italic>\n              -like 6-deoxyheptan capsular polysaccharide (CPS). These\n              <jats:italic>B. cepacia<\/jats:italic>\n              strains were initially identified based on their positive reactivity in a latex agglutination assay that uses the CPS-specific monoclonal antibody (mAb) 4B11. Mass spectrometry and\n              <jats:italic>recA<\/jats:italic>\n              sequencing confirmed the identity of these isolates as\n              <jats:italic>B. cepacia<\/jats:italic>\n              (formerly genomovar I). Total carbohydrates extracted from\n              <jats:italic>B. cepacia<\/jats:italic>\n              cell pellets reacted with\n              <jats:italic>B. pseudomallei<\/jats:italic>\n              CPS-specific mAbs MCA147, 3C5, and 4C4, but did not react with the\n              <jats:italic>B. pseudomallei<\/jats:italic>\n              lipopolysaccharide-specific mAb Pp-PS-W. Whole genome sequencing of the\n              <jats:italic>B. cepacia<\/jats:italic>\n              isolates revealed the presence of genes demonstrating significant homology to those comprising the\n              <jats:italic>B. pseudomallei<\/jats:italic>\n              CPS biosynthetic gene cluster. Collectively, our results provide compelling evidence that\n              <jats:italic>B. cepacia<\/jats:italic>\n              strains expressing the same CPS as\n              <jats:italic>B. pseudomallei<\/jats:italic>\n              co-exist in the environment alongside\n              <jats:italic>B. pseudomallei<\/jats:italic>\n              . Since CPS is a target that is often used for presumptive identification of\n              <jats:italic>B. pseudomallei<\/jats:italic>\n              , it is possible that the occurrence of these unique\n              <jats:italic>B. cepacia<\/jats:italic>\n              strains may complicate the diagnosis of melioidosis.\n            <\/jats:p>\n            <jats:sec>\n              <jats:title>IMPORTANCE<\/jats:title>\n              <jats:p>\n                <jats:italic>Burkholderia pseudomallei<\/jats:italic>\n                , the etiologic agent of melioidosis, is an important cause of morbidity and mortality in tropical and subtropical regions worldwide. The 6-deoxyheptan capsular polysaccharide (CPS) expressed by this bacterial pathogen is a promising target antigen that is useful for rapidly diagnosing melioidosis. Using assays incorporating CPS-specific monoclonal antibodies, we identified both clinical and environmental isolates of\n                <jats:italic>Burkholderia cepacia<\/jats:italic>\n                that express the same CPS antigen as\n                <jats:italic>B. pseudomallei<\/jats:italic>\n                . Because of this, it is important that staff working in melioidosis-endemic areas are aware that these strains co-exist in the same niches as\n                <jats:italic>B. pseudomallei<\/jats:italic>\n                and do not solely rely on CPS-based assays such as latex-agglutination, AMD Plus Rapid Tests, or immunofluorescence tests for the definitive identification of\n                <jats:italic>B. pseudomallei<\/jats:italic>\n                isolates.\n              <\/jats:p>\n            <\/jats:sec>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.03321-23","type":"journal-article","created":{"date-parts":[[2024,2,1]],"date-time":"2024-02-01T14:00:30Z","timestamp":1706796030000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":9,"title":["Identification of\n            <i>Burkholderia cepacia<\/i>\n            strains that express a\n            <i>Burkholderia pseudomallei<\/i>\n            -like capsular polysaccharide"],"prefix":"10.1128","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6763-4348","authenticated-orcid":true,"given":"Mary N.","family":"Burtnick","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, University of Nevada, Reno School of Medicine, Reno, Nevada, USA"},{"name":"Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9189-7244","authenticated-orcid":true,"given":"David A. 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