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This study screened 46 indole derivative agents for their antimicrobial activities against clinical isolates of extensively drug-resistant\n              <jats:italic>A. baumannii<\/jats:italic>\n              (XDRAB) with various degrees of biofilm production. Three selected indole agents\u20145-iodoindole, 3-methylindole, and 7-hydroxyindole\u2014were revealed to display potent antimicrobial and antibiofilm activity, including synergistic interplay with anti-\n              <jats:italic>A<\/jats:italic>\n              .\n              <jats:italic>baumannii<\/jats:italic>\n              antimicrobial drugs against XDRAB. Sub-inhibitory concentrations of these agents (particularly 7-hydroxyindole at 1\/64 of MIC) not only inhibited XDRAB biofilm formation but also eradicated the mature biofilm. The survival rate of XDRAB-infected\n              <jats:italic>Galleria mellonella<\/jats:italic>\n              was improved with the treatment of 7-hydroxyindole. Mechanistically, 7-hydroxyindole was found to reduce the expression of quorum sensing\/biofilm-implicated genes\n              <jats:italic>abaI<\/jats:italic>\n              and\n              <jats:italic>abaR<\/jats:italic>\n              . Together, the findings highlight the potential of indole derivatives against\n              <jats:italic>A. baumannii<\/jats:italic>\n              infections.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              Extensively drug-resistant\n              <jats:italic>Acinetobacter baumannii<\/jats:italic>\n              (XDRAB) isolates pose a major public health threat to antimicrobial therapy and are highly prevalent in hospital settings. This study identified and characterized indole derivative agents for their antimicrobial and antibiofilm activities against XDRAB. Sub-inhibitory indole agents such as 7-hydroxyindole can both inhibit XDRAB biofilm formation and eradicate the mature biofilm. Indole agents warrant further investigation against hard-to-treat antimicrobial-resistant pathogens.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.03388-24","type":"journal-article","created":{"date-parts":[[2025,4,15]],"date-time":"2025-04-15T11:23:01Z","timestamp":1744716181000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["Indole derivatives display antimicrobial and antibiofilm effects against extensively drug-resistant\n            <i>Acinetobacter baumannii<\/i>"],"prefix":"10.1128","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-5499-8255","authenticated-orcid":true,"given":"Junwei","family":"Li","sequence":"first","affiliation":[{"name":"Key Laboratory of Structure-Specific Small Molecule Drugs at Chengdu Medical College of Sichuan Province, School of Pharmacy, Chengdu Medical College","place":["Chengdu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-4753-8229","authenticated-orcid":false,"given":"Lulin","family":"Xie","sequence":"additional","affiliation":[{"name":"Key Laboratory of Structure-Specific Small Molecule Drugs at Chengdu Medical College of Sichuan Province, School of Pharmacy, Chengdu Medical College","place":["Chengdu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3489-1959","authenticated-orcid":false,"given":"Fei","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Clinical Medical College and The First Affiliated Hospital of Chengdu Medical College","place":["Chengdu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8896-4709","authenticated-orcid":true,"given":"Baodong","family":"Ling","sequence":"additional","affiliation":[{"name":"Key Laboratory of Structure-Specific Small Molecule Drugs at Chengdu Medical College of Sichuan Province, School of Pharmacy, Chengdu Medical College","place":["Chengdu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.9.2.148"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00058-07"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2017.148"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciad556"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1086\/500202"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-39658-3_13"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1017\/ice.2020.450"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.13718"},{"key":"e_1_3_3_10_2","unstructured":"WHO. 2024. 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