{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T17:54:31Z","timestamp":1785434071922,"version":"3.56.0"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T00:00:00Z","timestamp":1777939200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T00:00:00Z","timestamp":1777939200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01AI144016-01, R01AI172874-03"],"award-info":[{"award-number":["R01AI144016-01, R01AI172874-03"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["F32AI172084"],"award-info":[{"award-number":["F32AI172084"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["T32AI007414-32"],"award-info":[{"award-number":["T32AI007414-32"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"UW-Madison Food Research Institute"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2026,5,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Toxoplasma gondii<\/jats:italic>\n                      is a widespread parasite that impacts both human and animal health. Increasing use of organoid model systems has made previously challenging aspects of the\n                      <jats:italic toggle=\"yes\">T. gondii<\/jats:italic>\n                      lifecycle more accessible. The media for these organoid systems are highly complex with many growth factors and pathway inhibitors that promote stem cell retention or differentiation of the cells. We noticed changes in\n                      <jats:italic toggle=\"yes\">T. gondii<\/jats:italic>\n                      growth and development in our intestinal organoid system and wanted to determine if this was driven by cell type or media components. We found that low concentrations of SB202190 (a p38 MAPK inhibitor) and A83-01 (an ALK 4\/5\/7 receptor inhibitor) are each sufficient to alter\n                      <jats:italic toggle=\"yes\">T. gondii<\/jats:italic>\n                      growth even in fibroblast cells. Further investigation with our qPCR panel of\n                      <jats:italic toggle=\"yes\">T. gondii<\/jats:italic>\n                      stage markers revealed that these compounds promote bradyzoite cyst development and prime parasites for pre-sexual and sexual stage gene expression. As these complex organoid systems become more common in microbiology research, this study highlights the role of organoid media components in controlling pathogen growth and development.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      The use of complex cell culture model systems is becoming more common across many fields. Maintenance of these systems often includes growth factors and inhibitors not found in standard media. These added components have the potential to impact pathogen growth and development and can influence how experimental results may be interpreted. This study improves our understanding of the\n                      <jats:italic toggle=\"yes\">Toxoplasma gondii<\/jats:italic>\n                      lifecycle in one of those systems. It also serves as a template for other pathogen researchers to consider influences within their own systems.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.03472-25","type":"journal-article","created":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T13:00:46Z","timestamp":1775221246000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["Kinase inhibitors in organoid media influence\n                    <i>Toxoplasma gondii<\/i>\n                    growth and development"],"prefix":"10.1128","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-2742-7297","authenticated-orcid":false,"given":"Katie M.","family":"Cataldo","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01e4byj08","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Medical Microbiology & Immunology, University of Wisconsin-Madison","place":["Madison, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole M.","family":"Davis","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01e4byj08","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Medical Microbiology & Immunology, University of Wisconsin-Madison","place":["Madison, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9362-8088","authenticated-orcid":true,"given":"Laura J.","family":"Knoll","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01e4byj08","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Medical Microbiology & Immunology, University of Wisconsin-Madison","place":["Madison, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.1972.tb03431.x"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-37671-8"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3000364"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/dgd.12559"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2023.09.015"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.stemcr.2022.03.007"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-14875-7"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1099\/jgv.0.001629"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-022-05388-9"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0166-3542(99)00002-9"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0020-7519(02)00061-9"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00680-07"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004180"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaf5211"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002825"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2017.04.001"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/0065-2571(84)90007-4"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1124\/pr.58.3.10"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0012855"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2015.09.016"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1042\/0264-6021:3510095"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2005.00103.x"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.08.019"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1002093"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.21769\/BioProtoc.2728"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","unstructured":"Ulu A Srivastava S Kachour N White MW Wilson EH. 2025. 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