{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T14:26:56Z","timestamp":1788445616769,"version":"build-2803163510"},"reference-count":46,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2023,4,13]],"date-time":"2023-04-13T00:00:00Z","timestamp":1681344000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,4,13]],"date-time":"2023-04-13T00:00:00Z","timestamp":1681344000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Cultivation Fund Project of the National Natural Science Foundation in Beijing Children\u2019s Hospital, Capital Medical University","award":["GPMS201904"],"award-info":[{"award-number":["GPMS201904"]}]},{"DOI":"10.13039\/501100005090","name":"BMSTC | Beijing Nova Program","doi-asserted-by":"publisher","award":["Grant Nos. Z211100002121042"],"award-info":[{"award-number":["Grant Nos. Z211100002121042"]}],"id":[{"id":"10.13039\/501100005090","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004001","name":"Guizhou Science and Technology Department","doi-asserted-by":"publisher","award":["[2019]2822"],"award-info":[{"award-number":["[2019]2822"]}],"id":[{"id":"10.13039\/501100004001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004001","name":"Guizhou Science and Technology Department","doi-asserted-by":"publisher","award":["[2016]4021"],"award-info":[{"award-number":["[2016]4021"]}],"id":[{"id":"10.13039\/501100004001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2023,4,13]]},"abstract":"<jats:p>\n            The\n            <jats:named-content content-type=\"genus-species\">Mycobacterium tuberculosis<\/jats:named-content>\n            complex pathogen is a crucial infectious agent of tuberculosis. Hence, improving the capability of MTC detection is one of the most urgently required strategies for preventing and controlling TB.\n          <\/jats:p>","DOI":"10.1128\/spectrum.03475-22","type":"journal-article","created":{"date-parts":[[2023,3,28]],"date-time":"2023-03-28T14:10:10Z","timestamp":1680012610000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":19,"title":["Development of CRISPR\/Cas12b-Based Multiple Cross Displacement Amplification Technique for the Detection of\n            <i>Mycobacterium tuberculosis<\/i>\n            Complex in Clinical Settings"],"prefix":"10.1128","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4677-9470","authenticated-orcid":true,"given":"Xinggui","family":"Yang","sequence":"first","affiliation":[{"name":"Guizhou Provincial Center for Disease Control and Prevention, Guiyang, Guizhou, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junfei","family":"Huang","sequence":"additional","affiliation":[{"name":"Guizhou Provincial Center for Disease Control and Prevention, Guiyang, Guizhou, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yijiang","family":"Chen","sequence":"additional","affiliation":[{"name":"Guizhou Provincial Center for Disease Control and Prevention, Guiyang, Guizhou, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xia","family":"Ying","sequence":"additional","affiliation":[{"name":"Guizhou Provincial Center for Disease Control and Prevention, Guiyang, Guizhou, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinqin","family":"Tan","sequence":"additional","affiliation":[{"name":"Guizhou Provincial Center for Disease Control and Prevention, Guiyang, Guizhou, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1583-3916","authenticated-orcid":true,"given":"Xu","family":"Chen","sequence":"additional","affiliation":[{"name":"Second Affiliated Hospital, Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyan","family":"Zeng","sequence":"additional","affiliation":[{"name":"Second Affiliated Hospital, Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiguang","family":"Lei","sequence":"additional","affiliation":[{"name":"Guizhou Provincial Center for Disease Control and Prevention, Guiyang, Guizhou, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Wang","sequence":"additional","affiliation":[{"name":"Experimental Research Center, Capital Institute of Pediatrics, Beijing, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shijun","family":"Li","sequence":"additional","affiliation":[{"name":"Guizhou Provincial Center for Disease Control and Prevention, Guiyang, Guizhou, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms5812"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13073-020-00726-5"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.7860\/JCDR\/2016\/18837.7755"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00036-20"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2016.76"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00069-18"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(21)00435-X"},{"key":"e_1_3_3_9_2","unstructured":"World Health Organization. 2021. Global tuberculosis report 2021. WHO Geneva Switzerland."},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-020-05450-4"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI148013"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms7060177"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201646562"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0103091"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.708658"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1590\/1414-431X20143899"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biologicals.2020.01.007"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00812-17"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(06)70578-3"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.3855\/jidc.263"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00659-18"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1590\/S1678-9946202062036"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-021-02328-6"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.22655"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.02060-2020"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.681488"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2019.00078"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep11902"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.11.032"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1021\/acssensors.0c01984"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-24577-9"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abn7382"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.1c00686"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2021.743322"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00604-021-04985-w"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2021.122591"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaq0179"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aar6245"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-016-1232-6"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1007\/s42770-021-00520-4"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmyco.2016.09.025"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1590\/0074-02760160048"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2020.04.059"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.622389"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-08224-4"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2021.04.032"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2021.758564"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.03475-22","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.03475-22","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,13]],"date-time":"2023-04-13T13:27:50Z","timestamp":1681392470000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.03475-22"}},"subtitle":[],"editor":[{"given":"John Osei","family":"Sekyere","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2023,4,13]]},"references-count":46,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2023,4,13]]}},"alternative-id":["10.1128\/spectrum.03475-22"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.03475-22","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,13]]},"assertion":[{"value":"2022-08-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-03-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-03-28","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e03475-22"}}