{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T15:06:29Z","timestamp":1785423989450,"version":"3.56.0"},"reference-count":10,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T00:00:00Z","timestamp":1780358400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T00:00:00Z","timestamp":1780358400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2026,6,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      As the last line of defense against carbapenemase-producing Enterobacterales, the growing prevalence of tigecycline resistance poses a serious challenge to clinical therapy. Although\n                      <jats:italic toggle=\"yes\">tmexCD<\/jats:italic>\n                      -\n                      <jats:italic toggle=\"yes\">toprJ<\/jats:italic>\n                      clusters were initially described in Enterobacterales, increasing reports in non-fermenting gram-negative bacteria, including\n                      <jats:italic toggle=\"yes\">Pseudomonas<\/jats:italic>\n                      species, suggest potential cross-species dissemination (N. Dong, Y. Zeng, Y. Wang, C. Liu, J. Lu, C. Cai, X. Liu, Y. Chen, Y. Wu, Y. Fang, Y. Fu, Y. Hu, H. Zhou, J. Cai, F. Hu, S. Wang, Y. Wang, Y. Wu, G. Chen, Z. Shen, S. Chen, R. Zhang, Lancet Microbe 3:e846\u2013e856, 2022\n                      <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/doi.org\/10.1016\/S2666-5247(22)00221-X\" xlink:type=\"simple\">https:\/\/doi.org\/10.1016\/S2666-5247(22)00221-X<\/jats:ext-link>\n                      ). Here, we report a\n                      <jats:italic toggle=\"yes\">Pseudomonas sediminis<\/jats:italic>\n                      which exhibited reduced susceptibility to tigecycline (minimum inhibitory concentration &gt; 8 mg\/L) carrying\n                      <jats:italic toggle=\"yes\">tmexCD3<\/jats:italic>\n                      -t\n                      <jats:italic toggle=\"yes\">oprJ1<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>VIM-2<\/jats:sub>\n                      from a child\u2019s intestinal tract. Genomic analysis revealed that the gene cluster is present in different hosts and associated with a complex genetic environment.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      Tigecycline is regarded as one of the last options against multidrug-resistant gram-negative bacteria, especially carbapenemase-producing bacteria. Here, we describe a strain of\n                      <jats:italic toggle=\"yes\">Pseudomonas sediminis<\/jats:italic>\n                      (C3-T-78-1) in China carrying tigecycline resistance gene cluster\n                      <jats:italic toggle=\"yes\">tmexCD3<\/jats:italic>\n                      -\n                      <jats:italic toggle=\"yes\">toprJ1<\/jats:italic>\n                      and carbapenemase resistance\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>VIM-2<\/jats:sub>\n                      in a complex gene environment. We highlight that these genes have the potential to disseminate across bacterial hosts and may represent a potential reservoir for antimicrobial resistance dissemination.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.03491-25","type":"journal-article","created":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T13:04:16Z","timestamp":1777554256000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Co-occurrence of the tigecycline resistance gene cluster\n                    <i>tmexCD3-toprJ1<\/i>\n                    and verona integron-encoded metallo-\u03b2-lactamase (VIM) in a human-derived\n                    <i>Pseudomonas sediminis<\/i>\n                    isolate"],"prefix":"10.1128","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-0641-4552","authenticated-orcid":false,"given":"Heng","family":"Shen","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05gpas306","id-type":"ROR","asserted-by":"publisher"}],"name":"Hangzhou Medical College","place":["Hangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-4797-9524","authenticated-orcid":false,"given":"Aoxiao","family":"Chen","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00a2xv884","id-type":"ROR","asserted-by":"publisher"}],"name":"Clinical Microbiology Laboratory, The Second Affiliated Hospital of Zhejiang University School of Medicine, Zhejiang University","place":["Hangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang","family":"Cai","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05td3s095","id-type":"ROR","asserted-by":"publisher"}],"name":"Sanya Institute of Nanjing Agricultural University, Laboratory of Emerging Infectious Diseases and One Health, College of Veterinary Medicine, Nanjing Agricultural University","place":["Nanjing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2174-7985","authenticated-orcid":true,"given":"Rong","family":"Zhang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00a2xv884","id-type":"ROR","asserted-by":"publisher"}],"name":"Clinical Microbiology Laboratory, The Second Affiliated Hospital of Zhejiang University School of Medicine, Zhejiang University","place":["Hangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9478-4023","authenticated-orcid":true,"given":"Hongwei","family":"Zhou","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00a2xv884","id-type":"ROR","asserted-by":"publisher"}],"name":"Clinical Microbiology Laboratory, The Second Affiliated Hospital of Zhejiang University School of Medicine, Zhejiang University","place":["Hangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2613-7844","authenticated-orcid":true,"given":"Dazhi","family":"Jin","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05gpas306","id-type":"ROR","asserted-by":"publisher"}],"name":"Hangzhou Medical College","place":["Hangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02930-19"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02712-20"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S2666-5247(22)00221-X"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.envint.2024.109099"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13073-024-01399-0"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.4.891-897.2000"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2020.1768805"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.02928"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(12)70317-1"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11356-014-3632-y"}],"container-title":["Microbiology 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