{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T12:13:27Z","timestamp":1791807207421,"version":"4.3.4"},"reference-count":45,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2024,7,2]],"date-time":"2024-07-02T00:00:00Z","timestamp":1719878400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/doi.org\/10.1128\/AuthorWarrantyLicense.v1"},{"start":{"date-parts":[[2024,7,2]],"date-time":"2024-07-02T00:00:00Z","timestamp":1719878400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2024,7,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              <jats:italic>Candida auris<\/jats:italic>\n              , initially identified in 2009, has rapidly become a critical concern due to its antifungal resistance and significant mortality rates in healthcare-associated outbreaks. To date, whole-genome sequencing (WGS) has identified five unique clades of\n              <jats:italic>C. auris<\/jats:italic>\n              , with some strains displaying resistance to all primary antifungal drug classes. In this study, we presented the first WGS analysis of\n              <jats:italic>C. auris<\/jats:italic>\n              from Bangladesh, describing its origins, transmission dynamics, and antifungal susceptibility testing (AFST) profile. Ten\n              <jats:italic>C. auris<\/jats:italic>\n              isolates collected from hospital settings in Bangladesh were initially identified by CHROMagar Candida Plus, followed by VITEK2 system, and later sequenced using Illumina NextSeq 550 system. Reference-based phylogenetic analysis and variant calling pipelines were used to classify the isolates in different clades. All isolates aligned ~90% with the Clade I\n              <jats:italic>C. auris<\/jats:italic>\n              B11205 reference genome. Of the 10 isolates, 8 were clustered with Clade I isolates, highlighting a South Asian lineage prevalent in Bangladesh. Remarkably, the remaining two isolates formed a distinct cluster, exhibiting &gt;42,447 single-nucleotide polymorphism differences compared to their closest Clade IV counterparts. This significant variation corroborates the emergence of a sixth clade (Clade VI) of\n              <jats:italic>C. auris<\/jats:italic>\n              in Bangladesh, with potential for international transmission. AFST results showed that 80% of the\n              <jats:italic>C. auris<\/jats:italic>\n              isolates were resistant to fluconazole and voriconazole, whereas Clade VI isolates were susceptible to azoles, echinocandins, and pyrimidine analogue. Genomic sequencing revealed\n              <jats:italic>ERG11<\/jats:italic>\n              _Y132F mutation conferring azole resistance while\n              <jats:italic>FCY1<\/jats:italic>\n              _S70R mutation found inconsequential in describing 5-flucytosine resistance. Our study underscores the pressing need for comprehensive genomic surveillance in Bangladesh to better understand the emergence, transmission dynamics, and resistance profiles of\n              <jats:italic>C. auris<\/jats:italic>\n              infections. Unveiling the discovery of a sixth clade (Clade VI) accentuates the indispensable role of advanced sequencing methodologies.\n            <\/jats:p>\n            <jats:sec>\n              <jats:title>IMPORTANCE<\/jats:title>\n              <jats:p>\n                <jats:italic>Candida auris<\/jats:italic>\n                is a nosocomial fungal pathogen that is commonly misidentified as other\n                <jats:italic>Candida<\/jats:italic>\n                species. Since its emergence in 2009, this multidrug-resistant fungus has become one of the five urgent antimicrobial threats by 2019. Whole-genome sequencing (WGS) has proven to be the most accurate identification technique of\n                <jats:italic>C. auris<\/jats:italic>\n                which also played a crucial role in the initial discovery of this pathogen. WGS analysis of\n                <jats:italic>C. auris<\/jats:italic>\n                has revealed five distinct clades where isolates of each clade differ among themselves based on pathogenicity, colonization, infection mechanism, as well as other phenotypic characteristics. In Bangladesh,\n                <jats:italic>C. auris<\/jats:italic>\n                was first reported in 2019 from clinical samples of a large hospital in Dhaka city. To understand the origin, transmission dynamics, and antifungal-resistance profile of\n                <jats:italic>C. auris<\/jats:italic>\n                isolates circulating in Bangladesh, we conducted a WGS-based surveillance study on two of the largest hospital settings in Dhaka, Bangladesh.\n              <\/jats:p>\n            <\/jats:sec>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.03540-23","type":"journal-article","created":{"date-parts":[[2024,6,6]],"date-time":"2024-06-06T13:02:32Z","timestamp":1717678952000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":62,"title":["Emergence of the novel sixth\n            <i>Candida auris<\/i>\n            Clade VI in Bangladesh"],"prefix":"10.1128","volume":"12","author":[{"given":"Tahsin","family":"Khan","sequence":"first","affiliation":[{"name":"Genome Center, Infectious Diseases Division, icddr,b, Dhaka, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naimul Islam","family":"Faysal","sequence":"additional","affiliation":[{"name":"Genome Center, Infectious Diseases Division, icddr,b, Dhaka, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md Mobarok","family":"Hossain","sequence":"additional","affiliation":[{"name":"Genome Center, Infectious Diseases Division, icddr,b, Dhaka, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Syeda","family":"Mah-E-Muneer","sequence":"additional","affiliation":[{"name":"Emerging Infections, Infectious Diseases Division, icddr,b, Dhaka, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arefeen","family":"Haider","sequence":"additional","affiliation":[{"name":"Genome Center, Infectious Diseases Division, icddr,b, Dhaka, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shovan 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