{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T00:36:15Z","timestamp":1788568575234,"version":"build-2803163510"},"reference-count":23,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T00:00:00Z","timestamp":1722902400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T00:00:00Z","timestamp":1722902400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2024,8,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Mucormycosis is a severe and emerging invasive fungal infection associated with high mortality rates. Early diagnosis is crucial for initiating specific antifungal treatment, with molecular tools currently representing the most efficient diagnostic approach. Presently, a standardized in-house real-time PCR method is widely employed for diagnosing mucormycosis. Our study aimed to evaluate the agreement for the Mucorales DNA detection between two commercial real-time PCR assays\u2014the Fungiplex Mucorales Real-Time PCR Kit and the MycoGENIE\n                      <jats:italic>Aspergillus-Mucorales<\/jats:italic>\n                      spp. Real-Time PCR Kit\u2014in comparison with the in-house PCR. We retrospectively analyzed 58 samples previously identified as positive for Mucorales using the in-house PCR. These samples, obtained from 22 patients with proven or probable mucormycosis, were tested with both commercial kits. Additionally, samples from 40 patients without mucormycosis served as negative controls. Our findings revealed that the MycoGENIE Kit demonstrated superior performance in detecting Mucorales DNA in samples identified as positive by the in-house PCR. Notably, we observed minimal variability in cycle threshold (CT) values when comparing the results of the MycoGENIE Kit with those of the in-house PCR, with an average difference of 1.8 cycles. In contrast, the Fungiplex Kit exhibited a larger discrepancy in CT values compared to the in-house PCR, with an average difference of 4.1 cycles. The MycoGENIE Kit exhibited very good agreement (kappa of 0.82) with the in-house PCR for detecting Mucorales DNA across various sample types. These findings are important for the choice of kits that could be used to diagnose mucormycosis in clinical microbiology laboratories.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>Early diagnosis of mucormycosis is crucial for initiating effective treatment. The detection of Mucorales DNA by PCR in serum has revolutionized the diagnosis of this infection. However, the use of in-house methods can be time consuming. The availability of a commercial kit eliminates the need for in-house assay development, reducing laboratory workload and ensuring consistent performance across different healthcare settings. Currently, there are several commercial assays available, but many have limited evaluation. In this study, we compared two commercial kits and found that the MycoGENIE Kit offers a promising alternative to the in-house method.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.03585-23","type":"journal-article","created":{"date-parts":[[2024,6,25]],"date-time":"2024-06-25T09:01:32Z","timestamp":1719306092000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":13,"title":["Agreement between two real-time commercial PCR kits and an in-house real-time PCR for diagnosis of mucormycosis"],"prefix":"10.1128","volume":"12","author":[{"given":"Lovanirina Cl\u00e9mencia","family":"Rafanomezantsoa","sequence":"first","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9, Facult\u00e9 de M\u00e9decine, APHP, H\u00f4pital Necker Enfants-Malades, H\u00f4pital Europ\u00e9en Georges Pompidou, Unit\u00e9 de Parasitologie-Mycologie, Service de Microbiologie, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Estelle","family":"Sabourin","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9, Facult\u00e9 de M\u00e9decine, APHP, H\u00f4pital Necker Enfants-Malades, H\u00f4pital Europ\u00e9en Georges Pompidou, Unit\u00e9 de Parasitologie-Mycologie, Service de Microbiologie, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadia","family":"Guennouni Sebbouh","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9, Facult\u00e9 de M\u00e9decine, APHP, H\u00f4pital Necker Enfants-Malades, H\u00f4pital Europ\u00e9en Georges Pompidou, Unit\u00e9 de Parasitologie-Mycologie, Service de Microbiologie, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilie","family":"Sitterl\u00e9","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9, Facult\u00e9 de M\u00e9decine, APHP, H\u00f4pital Necker Enfants-Malades, H\u00f4pital Europ\u00e9en Georges Pompidou, Unit\u00e9 de Parasitologie-Mycologie, Service de Microbiologie, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nada","family":"Ben Halima","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9, Facult\u00e9 de M\u00e9decine, APHP, H\u00f4pital Necker Enfants-Malades, H\u00f4pital Europ\u00e9en Georges Pompidou, Unit\u00e9 de Parasitologie-Mycologie, Service de Microbiologie, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yannick Sonjah","family":"Raveloarisaona","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9, Facult\u00e9 de M\u00e9decine, APHP, H\u00f4pital Necker Enfants-Malades, H\u00f4pital Europ\u00e9en Georges Pompidou, Unit\u00e9 de Parasitologie-Mycologie, Service de Microbiologie, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilles","family":"Quesne","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9, Facult\u00e9 de M\u00e9decine, APHP, H\u00f4pital Necker Enfants-Malades, H\u00f4pital Europ\u00e9en Georges Pompidou, Unit\u00e9 de Parasitologie-Mycologie, Service de Microbiologie, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2817-3830","authenticated-orcid":true,"given":"Eric","family":"Dannaoui","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9, Facult\u00e9 de M\u00e9decine, APHP, H\u00f4pital Necker Enfants-Malades, H\u00f4pital Europ\u00e9en Georges Pompidou, Unit\u00e9 de Parasitologie-Mycologie, Service de Microbiologie, Paris, France"},{"name":"Dynamyc Research Group, Paris Est Cr\u00e9teil University (UPEC, EnvA), Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4721-2740","authenticated-orcid":false,"given":"Marie-Elisabeth","family":"Bougnoux","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9, Facult\u00e9 de M\u00e9decine, APHP, H\u00f4pital Necker Enfants-Malades, H\u00f4pital Europ\u00e9en Georges Pompidou, Unit\u00e9 de Parasitologie-Mycologie, Service de Microbiologie, Paris, France"},{"name":"Biologie et Pathog\u00e9nicit\u00e9 fongiques, D\u00e9partement de Mycologie, Institut Pasteur, Paris, 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