{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T09:12:49Z","timestamp":1769505169046,"version":"3.49.0"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2024,4,2]],"date-time":"2024-04-02T00:00:00Z","timestamp":1712016000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,4,2]],"date-time":"2024-04-02T00:00:00Z","timestamp":1712016000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2024,4,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              Neuroblastoma (NB) is a type of neuroectodermal tumor that originates from primitive sympathetic ganglion cells. Although many risk factors contributing to the occurrence of NB have been reported in recent years, the role of the gut microbiota in its development remains unclear. A bidirectional Mendelian randomization (MR) analysis was conducted to elucidate the causal relationship between the gut microbiota and NB. In the MR analysis, we employed the inverse-variance weighted (IVW) method as the primary criterion for assessing causality, while also utilizing three additional approaches, including MR-Egger, weighted median model, and weighted mode, for comprehensive evaluation. For gut microbiota that were causally associated with NB, a reverse MR was also used to assess the stability of this causal relationship. Finally, we also used external cohorts for validation and performed a meta-analysis of the results. The IVW results indicated a causal relationship between six gut microbiota and NB. Among the six gut microbiota, genus\n              <jats:italic>Lachnospiraceae<\/jats:italic>\n              [IVW odds ratio (OR): 2.66, 95% confidence interval (CI): 1.09\u20136.51,\n              <jats:italic>P<\/jats:italic>\n              value: 0.03] exhibited a detrimental effect against NB. On the other hand, the class\n              <jats:italic>Actinobacteria<\/jats:italic>\n              (IVW OR: 0.24, 95% CI: 0.07\u20130.77,\n              <jats:italic>P<\/jats:italic>\n              value: 0.02), the family\n              <jats:italic>Bifidobacteriaceae<\/jats:italic>\n              (IVW OR: 0.40, 95% CI: 0.17\u20130.96,\n              <jats:italic>P<\/jats:italic>\n              value: 0.04), the genus\n              <jats:italic>Desulfovibrio<\/jats:italic>\n              (IVW OR: 0.50, 95% CI: 0.25\u20130.97,\n              <jats:italic>P<\/jats:italic>\n              value: 0.04), the genus\n              <jats:italic>Bifidobacterium<\/jats:italic>\n              (IVW OR: 0.39, 95% CI: 0.16\u20130.92,\n              <jats:italic>P<\/jats:italic>\n              value: 0.03), and the genus\n              <jats:italic>Howardella<\/jats:italic>\n              (IVW OR: 0.55, 95% CI: 0.31\u20130.97,\n              <jats:italic>P<\/jats:italic>\n              value: 0.04) displayed a protective effect on NB. A reverse MR analysis did not reveal a causality between NB and the six gut microbiota. Meta-analysis showed that genus\n              <jats:italic>Bifidobacterium<\/jats:italic>\n              (meta OR: 0.41, 95% CI: 0.22\u20130.75,\n              <jats:italic>P<\/jats:italic>\n              &lt; 0.01) and genus\n              <jats:italic>Lachnospiraceae<\/jats:italic>\n              (meta OR: 2.20, 95% CI: 1.01\u20134.79,\n              <jats:italic>P<\/jats:italic>\n              &lt; 0.05) were still significant.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>Bidirectional Mendelian randomization was used to explore the causality between gut microbiota and neuroblastoma (NB). The results showed that there is a causal relationship between the six gut microbiota and NB, of which two gut microbiota were further confirmed in the meta-analysis. This may provide a new perspective on the prevention and treatment of NB.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.03656-23","type":"journal-article","created":{"date-parts":[[2024,2,27]],"date-time":"2024-02-27T14:01:40Z","timestamp":1709042500000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["The causal relationship between gut microbiota and neuroblastoma: a bidirectional Mendelian randomization analysis and meta-analysis"],"prefix":"10.1128","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2542-995X","authenticated-orcid":false,"given":"Zexin","family":"Zhang","sequence":"first","affiliation":[{"name":"The Second Clinical School of Guangzhou University of Chinese Medicine, Guangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dongting","family":"Li","sequence":"additional","affiliation":[{"name":"The Affiliated Guangzhou Hospital of TCM of Guangzhou University of Chinese Medicine, Guangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fengxi","family":"Xie","sequence":"additional","affiliation":[{"name":"Maoming Hospital of Guangzhou University of Chinese Medicine, Maoming, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8104-4280","authenticated-orcid":false,"given":"Haibo","family":"Zhang","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China"},{"name":"Guangdong Key Laboratory of Clinical Research of Chinese Medicine, Guangzhou, China"},{"name":"Guangdong Joint Laboratory of Guangdong, Hong Kong and Macao Chinese Medicine and Immune Diseases, Guangzhou, China"},{"name":"State Key Laboratory of Wet Certificate of Chinese Medicine Jointly Built by the Province and the Ministry, Guangzhou, 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