{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T09:37:13Z","timestamp":1769852233368,"version":"3.49.0"},"reference-count":74,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2022,12,21]],"date-time":"2022-12-21T00:00:00Z","timestamp":1671580800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2022,12,21]],"date-time":"2022-12-21T00:00:00Z","timestamp":1671580800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["P31093"],"award-info":[{"award-number":["P31093"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["M2867"],"award-info":[{"award-number":["M2867"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2022,12,21]]},"abstract":"<jats:p>In recent years, inducible promoters have gained increasing interest for industrial or laboratory use and have become key instruments for protein expression, synthetic biology, and metabolic engineering. Constitutive, high-expressing promoters can be used to achieve high expression yields; however, the continuous overexpression of specific proteins can lead to an unpredictable metabolic burden.<\/jats:p>","DOI":"10.1128\/spectrum.03670-22","type":"journal-article","created":{"date-parts":[[2022,11,9]],"date-time":"2022-11-09T14:01:14Z","timestamp":1668002474000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":18,"title":["Modular Inducible Multigene Expression System for Filamentous Fungi"],"prefix":"10.1128","volume":"10","author":[{"given":"Clara","family":"Baldin","sequence":"first","affiliation":[{"name":"Institute of Molecular Biology, Biocenter Innsbruck, Medical University of Innsbruck, Innsbruck, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"K\u00fchbacher","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology, Biocenter Innsbruck, Medical University of Innsbruck, Innsbruck, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Petra","family":"Merschak","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology, Biocenter Innsbruck, Medical University of Innsbruck, Innsbruck, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5952-9422","authenticated-orcid":true,"given":"Johannes","family":"Wagener","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, School of Medicine, Trinity College Dublin, The University of Dublin, Dublin, Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9257-8648","authenticated-orcid":true,"given":"Fabio","family":"Gsaller","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology, Biocenter Innsbruck, Medical University of Innsbruck, Innsbruck, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","unstructured":"Global Action for Fungal Infections. 2022. Burden of disease map. http:\/\/www.gaffi.org\/why\/burden-of-disease-maps. Accessed 20 July 2022."},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-016-8034-2"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3004404"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2013.09.007"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1186\/s40694-020-00095-z"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-009-2416-7"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2020.00293"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-018-0121-1"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1021\/acssynbio.1c00260"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2020.604923"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.3390\/jof7040257"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1080\/12298093.2020.1838115"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2005.06.002"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-394382-8.00001-0"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1100210108"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-018-9115-1"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12257-015-0567-1"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02999-09"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02740-10"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2015.11.003"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/8894215"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.644404"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1186\/s40694-018-0054-5"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003743"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2007\/007641-0"},{"key":"e_1_3_3_27_2","unstructured":"Global Action for Fungal Infections. 2022. Fungal disease frequency. https:\/\/gaffi.org\/why\/fungal-disease-frequency\/. Accessed 20 July 2022."},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.11.4810-4816.2000"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-5-1"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1271\/bbb.64.1416"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsle.2005.01.014"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jit530"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-005-0014-3"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1080\/13693780400029114"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2006.00370.x"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.3390\/jof6010012"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.7.2490-2496.2004"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00230-20"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.3390\/jof7070506"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(88)90377-0"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(92)16041-h"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201400584"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.02773"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02593-17"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22147739"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.03626-12"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.13167"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(03)01335-8"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-14941-6"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.3390\/toxins11120701"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms5030044"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1186\/s40694-016-0024-8"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-07112-1"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2013.11.003"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2013.10.036"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1984.tb02014.x"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0170404"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002530050779"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1996.00094.x"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1186\/s40694-017-0042-1"},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12926"},{"key":"e_1_3_3_62_2","doi-asserted-by":"publisher","DOI":"10.3390\/toxins9040126"},{"key":"e_1_3_3_63_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0061(199707)13:9<837::AID-YEA145>3.0.CO;2-T"},{"key":"e_1_3_3_64_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00351693"},{"key":"e_1_3_3_65_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.4.8.1328-1342.2005"},{"key":"e_1_3_3_66_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-18868-9"},{"key":"e_1_3_3_67_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi401618y"},{"key":"e_1_3_3_68_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006096"},{"key":"e_1_3_3_69_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00437-19"},{"key":"e_1_3_3_70_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky558"},{"key":"e_1_3_3_71_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-7799(89)90048-6"},{"key":"e_1_3_3_72_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkt075"},{"key":"e_1_3_3_73_2","doi-asserted-by":"crossref","unstructured":"Sastr\u00e9-Vel\u00e1squez LE Dallemulle A K\u00fchbacher A Baldin A Alcazar-Fuoli L Niedrig A Muller C Gsaller F. 2022. The fungal expel of 5-fluorocytosine derived fluoropyrimidines mitigates its antifungal activity and generates a cytotoxic environment. bioRxiv. doi:10.1101\/2022.08.24.504767.","DOI":"10.1101\/2022.08.24.504767"},{"key":"e_1_3_3_74_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0065-2660(08)60408-3"},{"key":"e_1_3_3_75_2","volume-title":"EUCAST definitive document E.Def.9.4: method for the determination of broth dilution minimum inhibitory concentrations of antifungal agents for conidia forming moulds","author":"Guinea J","year":"2022","unstructured":"Guinea J, Meletiadis J, Arikan-Akdagli S, Muehlethaler K, Kahlmeter G, Arendrup M. 2022. EUCAST definitive document E.Def.9.4: method for the determination of broth dilution minimum inhibitory concentrations of antifungal agents for conidia forming moulds. EUCAST, Vaxjo, Sweden."}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.03670-22","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.03670-22","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,21]],"date-time":"2022-12-21T14:29:44Z","timestamp":1671632984000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.03670-22"}},"subtitle":[],"editor":[{"given":"Gustavo H.","family":"Goldman","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2022,12,21]]},"references-count":74,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2022,12,21]]}},"alternative-id":["10.1128\/spectrum.03670-22"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.03670-22","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,12,21]]},"assertion":[{"value":"2022-09-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-10-19","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-11-09","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e03670-22"}}