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In this study, we demonstrated the involvement of XanB, an enzyme with phosphomannose isomerase (PMI) and guanosine diphosphate-mannose pyrophosphorylase (GMP) activities, in Xcc pathogenicity. Additionally, we found that XanB inhibitors protect the host against Xcc infection. Besides being deficient in motility, biofilm production, and ultraviolet resistance, the\n              <jats:italic>xanB<\/jats:italic>\n              deletion mutant was unable to cause disease, whereas\n              <jats:italic>xanB<\/jats:italic>\n              complementation restored wild-type phenotypes. XanB homology modeling allowed\n              <jats:italic>in silico<\/jats:italic>\n              virtual screening of inhibitors from databases, three of them being suitable in terms of absorption, distribution, metabolism, excretion, and toxicity (ADME\/Tox) properties, which inhibited GMP (but not PMI) activity of the Xcc recombinant XanB protein in more than 50%. Inhibitors reduced citrus canker severity up to 95%, similarly to copper-based treatment.\n              <jats:italic>xanB<\/jats:italic>\n              is essential for Xcc pathogenicity, and XanB inhibitors can be used for the citrus canker control.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              Xcc causes citrus canker, a threat to citrus production, which has been managed with copper, being required a more sustainable alternative for the disease control. XanB was previously found on the surface of Xcc, interacting with the host and displaying PMI and GMP activities. We demonstrated by\n              <jats:italic>xanB<\/jats:italic>\n              deletion and complementation that GMP activity plays a critical role in Xcc pathogenicity, particularly in biofilm formation. XanB homology modeling was performed, and\n              <jats:italic>in silico<\/jats:italic>\n              virtual screening led to carbohydrate-derived compounds able to inhibit XanB activity and reduce disease symptoms by 95%. XanB emerges as a promising target for drug design for control of citrus canker and other economically important diseases caused by\n              <jats:italic>Xanthomonas<\/jats:italic>\n              sp.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.03673-23","type":"journal-article","created":{"date-parts":[[2024,5,9]],"date-time":"2024-05-09T13:01:12Z","timestamp":1715259672000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["GDP-mannose pyrophosphorylase is an efficient target in\n            <i>Xanthomonas citri<\/i>\n            for citrus canker control"],"prefix":"10.1128","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0111-3491","authenticated-orcid":false,"given":"Andr\u00e9 Vessoni","family":"Alexandrino","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Bioqu\u00edmica e Biologia Molecular Aplicada (LBBMA), Departamento de 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