{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T13:12:26Z","timestamp":1770988346675,"version":"3.50.1"},"reference-count":20,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2024,3,5]],"date-time":"2024-03-05T00:00:00Z","timestamp":1709596800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,3,5]],"date-time":"2024-03-05T00:00:00Z","timestamp":1709596800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000897","name":"Cystic Fibrosis Foundation","doi-asserted-by":"crossref","award":["WHITEL20A0"],"award-info":[{"award-number":["WHITEL20A0"]}],"id":[{"id":"10.13039\/100000897","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000897","name":"Cystic Fibrosis Foundation","doi-asserted-by":"crossref","award":["WHITEL22G0"],"award-info":[{"award-number":["WHITEL22G0"]}],"id":[{"id":"10.13039\/100000897","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2024,3,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              The ESKAPEE pathogen\n              <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n              is a common cause of chronic wound and cystic fibrosis lung infections, as well as acute burn and nosocomial infections. Many of these infections are recalcitrant to conventional antibiotic therapies due to both traditional antibiotic resistance mechanisms and antimicrobial tolerance. Recent successes with bacteriophage (phage) therapy to treat chronic human\n              <jats:italic>P. aeruginosa<\/jats:italic>\n              infections have led to a renewed interest in isolating and characterizing new\n              <jats:italic>P. aeruginosa<\/jats:italic>\n              phages. Here, we isolated and characterized a new lytic phage (termed PIP, pili-infecting phage) capable of infecting\n              <jats:italic>P. aeruginosa<\/jats:italic>\n              PA14. PIP is a tailed phage with an icosahedral head and flexible tail containing a genome that is 57,462 bp in length. Phylogenetic analysis reveals that PIP belongs to the subfamily Queuovirinae and genus Nipunavirus but is highly divergent in gene content from known Nipunaviruses. By isolating and characterizing a\n              <jats:italic>P. aeruginosa<\/jats:italic>\n              strain that spontaneously evolved resistance to PIP, we show that the receptor for PIP is Type IV pili. In summary, we isolated a new\n              <jats:italic>P. aeruginosa<\/jats:italic>\n              phage species with a unique genome, thus increasing the diversity of phages known to infect this important human pathogen.\n            <\/jats:p>\n            <jats:sec>\n              <jats:title>IMPORTANCE<\/jats:title>\n              <jats:p>\n                The opportunistic pathogen\n                <jats:italic>Pseudomonas\u2009aeruginosa<\/jats:italic>\n                causes both acute and chronic human infections. These infections are notoriously difficult to treat due to both antibiotic resistance and antibiotic tolerance. The increasing frequency of antibiotic failure in\n                <jats:italic>P. aeruginosa<\/jats:italic>\n                infections has led scientists to explore other treatment options, including bacteriophage (phage) therapy. To this end, there has been a significant effort to identify new\n                <jats:italic>Pseudomonas<\/jats:italic>\n                phages. Here, we isolated and characterized a bacteriophage (termed PIP, pili-infecting phage) that infects\n                <jats:italic>P. aeruginosa<\/jats:italic>\n                PA14. Examination of the PIP genome revealed that this phage represents a new species in the subclass Queuovirinae. The isolation and characterization of spontaneous PA14 mutants that are resistant to PIP infection revealed Type IV pili as the PIP receptor. Ultimately, this study characterizes a new species of\n                <jats:italic>Pseudomonas<\/jats:italic>\n                phage, thus enhancing the known diversity of phages that infect this important pathogen.\n              <\/jats:p>\n            <\/jats:sec>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.03719-23","type":"journal-article","created":{"date-parts":[[2024,2,12]],"date-time":"2024-02-12T14:09:57Z","timestamp":1707746997000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["Characterization of a new\n            <i>Pseudomonas aeruginosa<\/i>\n            Queuovirinae bacteriophage"],"prefix":"10.1128","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-3118-8490","authenticated-orcid":false,"given":"Lauren E.","family":"Whiteley","sequence":"first","affiliation":[{"name":"School of Biological Sciences, Center for Microbial Dynamics and Infection, Georgia Institute of Technology, Atlanta, Georgia, USA"},{"name":"Emory-Children\u2019s Cystic Fibrosis Center, Atlanta, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4933-9983","authenticated-orcid":true,"given":"Marvin","family":"Whiteley","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, Center for Microbial Dynamics and Infection, Georgia Institute of Technology, Atlanta, Georgia, USA"},{"name":"Emory-Children\u2019s Cystic Fibrosis Center, Atlanta, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-021-01017-w"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01340-15"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0092-8674(03)00233-2"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/emph\/eoy005"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciac453"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00954-17"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-31837-9"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-39370-z"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.000860"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu153"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2021.689770"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btx440"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.3390\/v9040070"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.172.6.2911-2919.1990"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.16.4962-4969.1993"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-24-1-1"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-017-0061-y"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.62.6.1908-1912.1996"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2036282100"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(66)90317-5"}],"container-title":["Microbiology 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