{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T15:51:23Z","timestamp":1786117883205,"version":"build-2736575974"},"reference-count":67,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2026,8,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Klebsiella pneumoniae<\/jats:italic>\n                      sequence type 147 (ST147) is a globally disseminated multidrug-resistant (MDR) clone associated with hospital outbreaks, yet genomic data from low-income countries remain scarce. Here, we report the first genomic characterization of an extended-spectrum \u03b2-lactamase (ESBL)-producing\n                      <jats:italic toggle=\"yes\">K. pneumoniae<\/jats:italic>\n                      ST147 isolate (ARM07) recovered from a hospitalized patient in Armenia. Antimicrobial susceptibility testing revealed resistance to multiple antibiotic classes, while genomic analysis identified seven antimicrobial resistance (AMR) genes, namely,\n                      <jats:italic toggle=\"yes\">strA<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">strB<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sup>CTX-M-15<\/jats:sup>\n                      ,\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sup>SHV-11<\/jats:sup>\n                      ,\n                      <jats:italic toggle=\"yes\">sul2<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">qnrS1<\/jats:italic>\n                      , and\n                      <jats:italic toggle=\"yes\">catII.2,<\/jats:italic>\n                      consistent with its multidrug-resistant phenotype. Phylogenetic analysis showed that ARM07 clustered closely with nine Russian ST147 isolates, with divergence estimated around 2014 (95% CI: 2009 to 2018), suggesting a recent common ancestor. Compared with its Russian relatives, ARM07 carried fewer AMR and virulence determinants, implying lower selective pressure in Armenia. Nevertheless, ARM07 harbored a distinct plasmid profile, IncFIB(pKPHS1), IncR, IncFIA(pBK30683), and Col440I, and the insertion sequence (IS)\n                      <jats:italic toggle=\"yes\">ISKpn19<\/jats:italic>\n                      adjacent to AMR genes, indicating ongoing genomic plasticity and potential for further resistance acquisition. Our findings highlight the importance of genomic surveillance in Armenia and similar settings for monitoring the emergence and spread of high-risk\n                      <jats:italic toggle=\"yes\">K. pneumoniae<\/jats:italic>\n                      ST147 lineages, providing essential evidence to guide targeted antimicrobial stewardship and infection control strategies.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      We report the first in-depth genomic analysis of an extended-spectrum \u03b2-lactamase (ESBL)-producing\n                      <jats:italic toggle=\"yes\">Klebsiella pneumoniae<\/jats:italic>\n                      ST147 isolate (ARM07) recovered from a hospitalized patient in Armenia, a low- and middle-income country (LMIC) where genomic surveillance capacity remains severely limited. Antimicrobial susceptibility testing revealed resistance to multiple antibiotic classes, while genomic analysis identified seven antimicrobial resistance (AMR) genes, namely, strA, strB, blaCTX-M-15, blaSHV-11, sul2, qnrS1, and catII.2, consistent with its multidrug-resistant (MDR) phenotype. Phylogenetic analysis revealed that ARM07 shares a recent common ancestor with Russian ST147 isolates but has since diverged, possessing distinct AMR determinants, virulence features, and plasmid repertoire, likely shaped by local selective pressures. The identification of this high-risk clone in Armenia highlights the potential for regional dissemination of clinically important\n                      <jats:italic toggle=\"yes\">K. pneumoniae<\/jats:italic>\n                      lineages in settings with limited genomic surveillance capacity.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.03773-25","type":"journal-article","created":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T13:03:39Z","timestamp":1784207019000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Genomic characterization of an ESBL-producing\n                    <i>Klebsiella pneumoniae<\/i>\n                    ST147 recovered from a hospitalized patient in Armenia"],"prefix":"10.1128","volume":"14","author":[{"given":"Siyu","family":"Lu","sequence":"first","affiliation":[{"name":"Department of Paediatrics, Second Affiliated Hospital of the Army Medical University","place":["Chongqing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8196-8600","authenticated-orcid":false,"given":"Jie","family":"Sheng","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/017z00e58","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Basic Medical Sciences, Chongqing Medical University","place":["Chongqing, China"]},{"name":"The Joint International Research Laboratory of Reproduction and Development, Ministry of Education","place":["Chongqing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary M.","family":"Ter-Stepanyan","sequence":"additional","affiliation":[{"name":"Faculty of Public Health, Department of Epidemiology, Yerevan State Medical University after M. Heratsi","place":["Yerevan, Republic of Armenia"]},{"name":"Research Center of Maternal and Child Health Protection","place":["Yerevan, Armenia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingxiong","family":"Wang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/017z00e58","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Basic Medical Sciences, Chongqing Medical University","place":["Chongqing, China"]},{"name":"The Joint International Research Laboratory of Reproduction and Development, Ministry of Education","place":["Chongqing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7339-7297","authenticated-orcid":true,"given":"Hermine V.","family":"Mkrtchyan","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03e5mzp60","id-type":"ROR","asserted-by":"publisher"}],"name":"Centre for Innovation for Genomics and Microbiome Sciences, University of West London","place":["London, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-019-0315-1"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13756-025-01614-6"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.7189\/jogh.15.04198"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2025.1650010"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.835417"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/femsre\/fux013"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2023.10.019"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2021.02.020"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1136\/archdischild-2015-308707"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms12122548"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2024.1348093"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12941-019-0343-8"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.h6420"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkn287"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkp264"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1186\/1757-4749-4-7"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.06142-11"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2011.03532.x"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01686-12"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00418-17"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1007\/s40121-024-01097-9"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.03416-23"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-025-03798-8"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2017.22.49.17-00048"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.es2015.20.23.21150"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.01859"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01148-20"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1099\/mgen.0.000737"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1111\/nyas.13310"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.04011-22"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.01910-24"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.00332-25:e0033225"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-024-10368-2"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2022.27.50.2200926"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkx216"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.3109\/1040841X.2012.691460"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.4161\/viru.22974"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens3030743"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2025.1581851"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2025.151666"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02749-13"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S212321"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2025.1622352"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-022-08564-y"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ram.2024.02.007"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.1002\/mbo3.1349"},{"key":"e_1_3_4_48_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.743981"},{"key":"e_1_3_4_49_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0054223"},{"key":"e_1_3_4_50_2","unstructured":"The European committee on antimicrobial susceptibility testing. 2021. Breakpoint tables for interpretation of MICs and zone diameters. Version 11.0. Available from: http:\/\/www.eucast.org"},{"key":"e_1_3_4_51_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu170"},{"key":"e_1_3_4_52_2","doi-asserted-by":"publisher","DOI":"10.1002\/cpbi.102"},{"key":"e_1_3_4_53_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu153"},{"key":"e_1_3_4_54_2","doi-asserted-by":"publisher","DOI":"10.12688\/wellcomeopenres.14826.1"},{"key":"e_1_3_4_55_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-24448-3"},{"key":"e_1_3_4_56_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab1107"},{"key":"e_1_3_4_57_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02412-14"},{"key":"e_1_3_4_58_2","doi-asserted-by":"publisher","DOI":"10.1099\/mgen.0.000206"},{"key":"e_1_3_4_59_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciab784"},{"key":"e_1_3_4_60_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku1196"},{"key":"e_1_3_4_61_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0009490"},{"key":"e_1_3_4_62_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab301"},{"key":"e_1_3_4_63_2","doi-asserted-by":"publisher","DOI":"10.12688\/wellcomeopenres.14694.1"},{"key":"e_1_3_4_64_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv421"},{"key":"e_1_3_4_65_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-016-1108-8"},{"key":"e_1_3_4_66_2","doi-asserted-by":"publisher","DOI":"10.1039\/C5AY02550H"},{"key":"e_1_3_4_67_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2148-7-214"},{"key":"e_1_3_4_68_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2109"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.03773-25","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.03773-25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T14:40:32Z","timestamp":1786027232000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.03773-25"}},"subtitle":[],"editor":[{"given":"Gabriele","family":"Arcari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,8,4]]},"references-count":67,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8,4]]}},"alternative-id":["10.1128\/spectrum.03773-25"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.03773-25","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,4]]},"assertion":[{"value":"2025-11-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e03773-25"}}