{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T02:54:31Z","timestamp":1791946471325,"version":"4.3.4"},"reference-count":33,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T00:00:00Z","timestamp":1780358400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"SCINet project and the AI Center of Excellence of the USDA Agricultural Research Service","award":["0500-00093-001-00-D"],"award-info":[{"award-number":["0500-00093-001-00-D"]}]},{"DOI":"10.13039\/100008678","name":"Center for Produce Safety","doi-asserted-by":"crossref","award":["2024CPS02"],"award-info":[{"award-number":["2024CPS02"]}],"id":[{"id":"10.13039\/100008678","id-type":"DOI","asserted-by":"crossref"}]},{"name":"U.S. Department of Agriculture, Agricultural Research Service, and Food Safety Program","award":["8072-41420-025"],"award-info":[{"award-number":["8072-41420-025"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2026,6,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Listeria monocytogenes<\/jats:italic>\n                      contamination of fresh-cut produce poses a threat to consumers due to its high mortality rate in humans. Because it is widespread in the environment, as well as able to grow and survive in harsh conditions, such as low temperatures, it is difficult to control. A strain of\n                      <jats:italic toggle=\"yes\">L. monocytogenes<\/jats:italic>\n                      , L008, with significantly faster growth than other strains, was identified in our laboratory during experiments with fresh-cut produce. The L008 strain had a consistently shorter lag phase and reached maximum spectrophotometric absorbance (optical density) faster than three other\n                      <jats:italic toggle=\"yes\">L. monocytogenes<\/jats:italic>\n                      strains during growth curve studies at 4 and 8\u00b0C. The lag phase was also shorter when compared with two of the three strains at 12\u00b0C. A genetic comparison between L008 and the other three strains identified missense mutations in the\n                      <jats:italic toggle=\"yes\">fapR, mngB, pfkB,<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">uvrC<\/jats:italic>\n                      genes of L008 that may account for the faster growth. These genes are involved in metabolism, cell membrane maintenance, and stress tolerance, which may allow L008 to adapt to lower temperatures better than the other strains. Accelerated growth at low temperatures may elevate the risk associated with this isolate and other\n                      <jats:italic toggle=\"yes\">L. monocytogenes<\/jats:italic>\n                      strains with similar genetic variants. This study highlights the importance of including bacterial isolates that demonstrate a worst-case scenario for lag phase or more rapid growth in foodborne pathogen challenge studies.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Listeria monocytogenes<\/jats:italic>\n                      is responsible for listeriosis in humans and has a relatively high incidence of hospitalization and mortality when compared to other foodborne pathogens. The psychrotrophic nature of\n                      <jats:italic toggle=\"yes\">L. monocytogenes<\/jats:italic>\n                      allows it to grow at low temperatures, makes it difficult to control, and we identified an isolate with faster growth at 4, 8, and 12\u00b0C. A comparison of the isolate\u2019s genome with three other\n                      <jats:italic toggle=\"yes\">L. monocytogenes<\/jats:italic>\n                      strains found changes in genes involved in energy usage, cell membrane maintenance, and stress resistance. These genetic differences may allow the isolate to adjust to lower temperatures better than other strains and replicate more quickly. The ability to grow faster at low temperatures could increase the risk of foodborne illness from this isolate and other\n                      <jats:italic toggle=\"yes\">L. monocytogenes<\/jats:italic>\n                      with similar genetic changes.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.03786-25","type":"journal-article","created":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T11:18:31Z","timestamp":1777547911000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["Genetic variations and accelerated growth at low temperatures in a\n                    <i>Listeria monocytogenes<\/i>\n                    isolate"],"prefix":"10.1128","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9953-8676","authenticated-orcid":true,"given":"Katrina L.","family":"Counihan","sequence":"first","affiliation":[{"name":"United States Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center","place":["Wyndmoor, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nirosha Ruwani","family":"Amarasekara","sequence":"additional","affiliation":[{"name":"United States Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center","place":["Wyndmoor, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5844-7794","authenticated-orcid":false,"given":"Joshua B.","family":"Gurtler","sequence":"additional","affiliation":[{"name":"United States Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center","place":["Wyndmoor, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1656-7522","authenticated-orcid":false,"given":"Xuetong","family":"Fan","sequence":"additional","affiliation":[{"name":"United States Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center","place":["Wyndmoor, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.006774"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/fsn3.2910"},{"key":"e_1_3_2_4_2","unstructured":"Clinical overview of listeriosis. 2024. Available from: https:\/\/www.cdc.gov\/listeria\/hcp\/clinical-overview\/index.html"},{"key":"e_1_3_2_5_2","unstructured":"FoodNet Fast. 2025. Available from: https:\/\/wwwn.cdc.gov\/foodnetfast"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid3104.240913"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/cmr.00060-19"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-60.11.1334"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0168-1605(96)01224-x"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.738470"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.792162"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2021.01.008"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2025.111451"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkae410"},{"key":"e_1_3_2_15_2","doi-asserted-by":"crossref","unstructured":"Counihan KL et al.. 2026. Data from: genetic variations and accelerated growth at low temperatures in a Listeria monocytogenes isolate. Ag Data Commons","DOI":"10.1128\/spectrum.03786-25"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41597-025-05356-3"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/humu.20938"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/s1534-5807(03)00123-0"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1839"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2014.00068"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2016.00064"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1006\/fmic.1997.0170"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00980-07"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0009006"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr401004a"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.syapm.2008.05.002"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1472-765x.2000.00646.x"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20051183"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-015-1116-1"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.dnarep.2007.09.002"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2017.06.008"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028x-69.12.3031"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.00486-22"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0305663"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.03786-25","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.03786-25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T13:08:16Z","timestamp":1781701696000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.03786-25"}},"subtitle":[],"editor":[{"given":"Salina","family":"Parveen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,6,2]]},"references-count":33,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,6,2]]}},"alternative-id":["10.1128\/spectrum.03786-25"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.03786-25","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,2]]},"assertion":[{"value":"2025-11-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-03","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e03786-25"}}