{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T15:52:23Z","timestamp":1786117943688,"version":"build-2736575974"},"reference-count":81,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Sichuan Province Transportation Science and Technology Project","award":["2019-ZL-19"],"award-info":[{"award-number":["2019-ZL-19"]}]},{"name":"Natural Science Foundation of Sichuan Provence","award":["2023NSFSC0143"],"award-info":[{"award-number":["2023NSFSC0143"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31800458"],"award-info":[{"award-number":["31800458"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2026,8,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>Wetlands are among the world\u2019s most significant ecosystems. Studying how different grazing intensities affect soil bacterial diversity and community assembly in alpine wetlands is crucial for their sustainable management. Based on 16S sequencing technology, this study analyzed the differences in the composition, structure, and community assembly mechanisms of bacterial communities under different grazing impacts on the Qinghai-Tibet Plateau. The research results showed that under the influence of grazing, there was no significant difference in the \u03b1-diversity of the soil bacterial community, but a certain degree of reorganization occurred at the phylum level of bacteria. At the same grazing intensity, the relative abundances of different bacterial phyla in the wetland were different, and at different grazing intensities, the relative abundances of the same phylum might have significant differences. Co-occurrence network analysis indicated that wetlands with medium grazing intensity had a higher degree of modularity. The results of the null model and neutral model showed that, with the increase in grazing intensity, the assembly process of the soil bacterial community gradually shifted from being dominated by deterministic processes to being dominated by neutral processes, with a decrease in heterogeneous selection and an increase in dispersal limitation.<\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>This work innovatively clarifies grazing\u2019s regulatory effects on alpine wetland soil bacterial community assembly, revealing the deterministic-to-neutral shift with increasing grazing intensity and higher modularity under moderate grazing. It deepens understanding of \u201cdisturbance-microbial assembly\u201d relationships, bridging environmental science, disturbance ecology, and microbiology. Practically, it provides a scientific basis for optimizing grazing management to maintain microbial function, crucial for sustainable management of Qinghai-Tibet alpine wetlands and similar ecosystems.<\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.03791-25","type":"journal-article","created":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T13:18:54Z","timestamp":1783343934000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Bacterial diversity and community assembly mechanisms in alpine wetland soils under the influence of grazing"],"prefix":"10.1128","volume":"14","author":[{"given":"Xu","family":"Ai","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04s99y476","id-type":"ROR","asserted-by":"publisher"}],"name":"Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), China West Normal University","place":["Nanchong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cao","family":"Yu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04s99y476","id-type":"ROR","asserted-by":"publisher"}],"name":"Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), China West Normal University","place":["Nanchong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji","family":"Yangyue","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04s99y476","id-type":"ROR","asserted-by":"publisher"}],"name":"Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), China West Normal University","place":["Nanchong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zou","family":"Shuzhen","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04s99y476","id-type":"ROR","asserted-by":"publisher"}],"name":"Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), China West Normal University","place":["Nanchong, China"]},{"id":[{"id":"https:\/\/ror.org\/04s99y476","id-type":"ROR","asserted-by":"publisher"}],"name":"Laboratory of Environmental Science and Biodiversity Conservation, China West Normal University","place":["Nanchong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4255-8689","authenticated-orcid":true,"given":"Kang","family":"Di","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04s99y476","id-type":"ROR","asserted-by":"publisher"}],"name":"Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), China West Normal University","place":["Nanchong, China"]},{"id":[{"id":"https:\/\/ror.org\/04s99y476","id-type":"ROR","asserted-by":"publisher"}],"name":"Laboratory of Environmental Science and Biodiversity Conservation, China West Normal University","place":["Nanchong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.3390\/agronomy14040669"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2018.03.373"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.11829\/j.issn.1001-0629.2022-0682"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2020.144038"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecolind.2021.107783"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/ldr.4987"},{"key":"e_1_3_4_8_2","volume-title":"Restoration of wetland ecosystem: a trajectory towards a sustainable environment","author":"Yadav N","year":"2019","unstructured":"Yadav N, Singh DP. 2019. Microalgae and microorganisms: important regulators of carbon dynamics in wetland ecosystem. In Restoration of wetland ecosystem: a trajectory towards a sustainable environment. Springer, Singapore."},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/ecy.1879"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.17031"},{"key":"e_1_3_4_11_2","first-page":"116","article-title":"Research progress on influencing factors of soil microbial biomass and diversity","volume":"8","author":"Zhang Q","year":"2022","unstructured":"Zhang Q, Wang C, Sun Z, Li S, Liang Y. 2022. Research progress on influencing factors of soil microbial biomass and diversity. Northern Hortic 8:116\u2013121.","journal-title":"Northern Hortic"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-2920.2003.00477.x"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1890\/0012-9658(2001)082[2397:CPSSMA]2.0.CO;2"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.agee.2023.108541"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.13287\/j.1001-9332.201909.010"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0038-0717(98)00052-2"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.soilbio.2004.10.014"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/gcb.13080"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/oik.01493"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1262073"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/mec.14914"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-016-7802-3"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tree.2011.03.024"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00002-17"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2014.06.088"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0601602103"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-52734-0"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/nclimate3299"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2021.148363"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.still.2024.106033"},{"key":"e_1_3_4_31_2","first-page":"2","article-title":"Grassland degradation and control strategies in northern China","volume":"30","author":"Li B","year":"1997","unstructured":"Li B. 1997. Grassland degradation and control strategies in northern China. Sci Agric Sin 30:2\u201310.","journal-title":"Sci Agric Sin"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2025.112494"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","unstructured":"Oksanen J Blanchet FG Friendly M Kindt R Legendre R McGlinn D Minchin P O\u2019Hara RB Simpson GL Solymos P Stevens MHH Szoecs E Wagner H. 2022. Vegan: community ecology package. R package version 2.6-4. doi:10.32614\/CRAN.package.vegan","DOI":"10.32614\/CRAN.package.vegan"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2013.93"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2023.168627"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1002\/ldr.5601"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.13817\/j.cnki.cyycp.2024.05.030"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.13287\/j.1001-9332.201705.032"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.geoderma.2021.115035"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.soilbio.2020.107817"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41396-020-0669-4"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1159\/000516215"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.agee.2024.109190"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.soilbio.2015.10.024"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2024.173662"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0213844"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1004041"},{"key":"e_1_3_4_48_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00373-10"},{"key":"e_1_3_4_49_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1016303213326"},{"key":"e_1_3_4_50_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.741645"},{"key":"e_1_3_4_51_2","doi-asserted-by":"publisher","DOI":"10.1029\/2020JG006027"},{"key":"e_1_3_4_52_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep40093"},{"key":"e_1_3_4_53_2","first-page":"EP33D","volume-title":"AGU fall meeting abstracts 2016","author":"Chen L","year":"2016","unstructured":"Chen L, Wu S, Minghui Y. 2016. How does slope gradient affect runoff and soil erosion on hillslopes? AGU fall meeting abstracts 2016. p EP33D\u20131033"},{"key":"e_1_3_4_54_2","doi-asserted-by":"publisher","DOI":"10.3390\/f10070601"},{"key":"e_1_3_4_55_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jenvman.2022.115770"},{"key":"e_1_3_4_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsoil.2018.11.003"},{"key":"e_1_3_4_57_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-33598-8_21-1"},{"key":"e_1_3_4_58_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11368-017-1680-x"},{"key":"e_1_3_4_59_2","doi-asserted-by":"publisher","DOI":"10.1088\/1755-1315\/648\/1\/012143"},{"key":"e_1_3_4_60_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-2508(03)00082-2"},{"key":"e_1_3_4_61_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms13010138"},{"key":"e_1_3_4_62_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00343-021-1214-7"},{"key":"e_1_3_4_63_2","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-018-0497-1"},{"key":"e_1_3_4_64_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsoil.2019.03.001"},{"key":"e_1_3_4_65_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.catena.2023.107389"},{"key":"e_1_3_4_66_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.00261"},{"key":"e_1_3_4_67_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.agee.2024.108915"},{"key":"e_1_3_4_68_2","doi-asserted-by":"publisher","DOI":"10.1093\/femsec\/fiae017"},{"key":"e_1_3_4_69_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13717-024-00516-6"},{"key":"e_1_3_4_70_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature00812"},{"key":"e_1_3_4_71_2","doi-asserted-by":"publisher","DOI":"10.1002\/imt2.18"},{"key":"e_1_3_4_72_2","doi-asserted-by":"publisher","DOI":"10.3390\/fishes8050224"},{"key":"e_1_3_4_73_2","doi-asserted-by":"publisher","DOI":"10.1111\/ele.70173"},{"key":"e_1_3_4_74_2","doi-asserted-by":"publisher","DOI":"10.12160\/j.issn.1672-5190.2021.03.014"},{"key":"e_1_3_4_75_2","doi-asserted-by":"publisher","DOI":"10.1002\/ldr.2501"},{"key":"e_1_3_4_76_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.00606"},{"key":"e_1_3_4_77_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01372-16"},{"key":"e_1_3_4_78_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.soilbio.2018.08.029"},{"key":"e_1_3_4_79_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.agee.2025.109753"},{"key":"e_1_3_4_80_2","doi-asserted-by":"publisher","DOI":"10.1111\/nph.14606"},{"key":"e_1_3_4_81_2","doi-asserted-by":"publisher","DOI":"10.1111\/nph.15161"},{"key":"e_1_3_4_82_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-20271-4"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.03791-25","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.03791-25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T14:42:08Z","timestamp":1786027328000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.03791-25"}},"subtitle":[],"editor":[{"given":"Warish","family":"Ahmed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,8,4]]},"references-count":81,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8,4]]}},"alternative-id":["10.1128\/spectrum.03791-25"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.03791-25","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,4]]},"assertion":[{"value":"2025-11-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e03791-25"}}