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Nevertheless, its widespread application has been hindered by its comparatively slow efficacy in eliminating mosquitoes. To augment the potency of\n                      <jats:italic>B. bassiana<\/jats:italic>\n                      against\n                      <jats:italic>Aedes<\/jats:italic>\n                      mosquitoes, a novel recombinant strain,\n                      <jats:italic>Bb<\/jats:italic>\n                      -Cyt1Aa, was developed by incorporating the\n                      <jats:italic>Bacillus thuringiensis<\/jats:italic>\n                      toxin gene\n                      <jats:italic>Cyt1Aa<\/jats:italic>\n                      into\n                      <jats:italic>B. bassiana<\/jats:italic>\n                      . The virulence of\n                      <jats:italic>Bb<\/jats:italic>\n                      -Cyt1Aa was evaluated against\n                      <jats:italic>Aedes aegypti<\/jats:italic>\n                      and\n                      <jats:italic>Aedes albopictus<\/jats:italic>\n                      using insect bioassays. Compared to the wild-type (WT) strain, the median lethal time (LT\n                      <jats:sub>50<\/jats:sub>\n                      ) for\n                      <jats:italic>A. aegypti<\/jats:italic>\n                      larvae infected with\n                      <jats:italic>Bb<\/jats:italic>\n                      -Cyt1Aa decreased by 33.3% at a concentration of 1 \u00d7 10\n                      <jats:sup>8<\/jats:sup>\n                      conidia\/mL and by 22.2% at 1 \u00d7 10\n                      <jats:sup>7<\/jats:sup>\n                      conidia\/mL. The LT\n                      <jats:sub>50<\/jats:sub>\n                      for\n                      <jats:italic>A. aegypti<\/jats:italic>\n                      adults infected with\n                      <jats:italic>Bb<\/jats:italic>\n                      -Cyt1Aa through conidia ingestion was reduced by 37.5% at 1 \u00d7 10\n                      <jats:sup>8<\/jats:sup>\n                      conidia\/mL and by 33.3% at 1 \u00d7 10\n                      <jats:sup>7<\/jats:sup>\n                      conidia\/mL. Likewise, the LT\n                      <jats:sub>50<\/jats:sub>\n                      for\n                      <jats:italic>A. aegypti<\/jats:italic>\n                      adults infected with\n                      <jats:italic>Bb<\/jats:italic>\n                      -Cyt1Aa through cuticle contact decreased by 33.3% and 30.8% at the same concentrations, respectively. Furthermore, the\n                      <jats:italic>Bb<\/jats:italic>\n                      -Cyt1Aa strain also demonstrated increased toxicity against both larval and adult\n                      <jats:italic>A. albopictus<\/jats:italic>\n                      , when compared to the WT strain. In conclusion, our study demonstrated that the expression of\n                      <jats:italic>B. thuringiensis<\/jats:italic>\n                      toxin Cyt1Aa in\n                      <jats:italic>B. bassiana<\/jats:italic>\n                      enhanced its virulence against\n                      <jats:italic>Aedes<\/jats:italic>\n                      mosquitoes. This suggests that\n                      <jats:italic>B. bassiana<\/jats:italic>\n                      expressing Cyt1Aa has potential value for use in mosquito control.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Beauveria bassiana<\/jats:italic>\n                      is a naturally occurring fungus that can be utilized as a bioinsecticide against mosquitoes. Cyt1Aa is a delta-endotoxin protein produced by\n                      <jats:italic>Bacillus thuringiensis<\/jats:italic>\n                      that exhibits specific and potent insecticidal activity against mosquitoes. In our study, the expression of this toxin Cyt1Aa in\n                      <jats:italic>B. bassiana<\/jats:italic>\n                      enhances the virulence of\n                      <jats:italic>B. bassiana<\/jats:italic>\n                      against\n                      <jats:italic>Aedes aegypti<\/jats:italic>\n                      and\n                      <jats:italic>Aedes albopictus<\/jats:italic>\n                      , thereby increasing their effectiveness in killing mosquitoes. This novel strain can be used alongside chemical insecticides to reduce dependence on harmful chemicals, thereby minimizing negative impacts on the environment and human health. Additionally, the potential resistance of\n                      <jats:italic>B. bassiana<\/jats:italic>\n                      against mosquitoes in the future could be overcome by acquiring novel combinations of exogenous toxin genes. The presence of\n                      <jats:italic>B. bassiana<\/jats:italic>\n                      that expresses Cyt1Aa is of significant importance in mosquito control as it enhances genetic diversity, creates novel virulent strains, and contributes to the development of safer and more sustainable methods of mosquito control.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.03792-23","type":"journal-article","created":{"date-parts":[[2024,5,29]],"date-time":"2024-05-29T09:10:58Z","timestamp":1716973858000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["Recombinant\n                    <i>Beauveria bassiana<\/i>\n                    expressing\n                    <i>Bacillus thuringiensis<\/i>\n                    toxin Cyt1Aa: a promising approach for enhancing\n                    <i>Aedes<\/i>\n                    mosquito control"],"prefix":"10.1128","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0901-7661","authenticated-orcid":true,"given":"Sheng-Qun","family":"Deng","sequence":"first","affiliation":[{"name":"Department of Pathogen Biology, School of Basic Medical Sciences, Anhui Medical University, Hefei, China"},{"name":"Anhui Province Key Laboratory of Zoonoses, the Key Laboratory of Zoonoses of High Institutions in Anhui, Anhui Medical University, Hefei, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ni","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pathogen Biology, School of Basic Medical Sciences, Anhui Medical University, Hefei, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu-Ke","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Pathogen Biology, School of Basic Medical Sciences, Anhui Medical University, Hefei, China"},{"name":"Anhui Province Key Laboratory of Zoonoses, the Key Laboratory of Zoonoses of High Institutions in Anhui, Anhui Medical University, Hefei, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong-Zheng","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Pathogen Biology, School of Basic Medical Sciences, Anhui Medical University, Hefei, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia-Hua","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pathogen Biology, School of Basic Medical Sciences, Anhui Medical University, Hefei, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhe-Yu","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of Pathogen Biology, School of Basic Medical Sciences, Anhui Medical University, Hefei, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin-Min","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pathogen Biology, School of Basic Medical Sciences, Anhui Medical University, Hefei, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pathogen Biology, School of Basic Medical Sciences, Anhui Medical University, Hefei, China"},{"name":"Anhui Province Key Laboratory of Zoonoses, the Key Laboratory of Zoonoses of High Institutions in Anhui, Anhui Medical University, Hefei, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5342-2803","authenticated-orcid":false,"given":"Chao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pathogen Biology, School of Basic Medical Sciences, Anhui Medical University, Hefei, China"},{"name":"Anhui Province Key Laboratory of Zoonoses, the Key Laboratory of Zoonoses of High Institutions in Anhui, Anhui Medical University, Hefei, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0499-2073","authenticated-orcid":false,"given":"Chen","family":"Chen","sequence":"additional","affiliation":[{"name":"Anhui Province Key Laboratory of Zoonoses, the Key Laboratory of Zoonoses of High Institutions in Anhui, Anhui Medical University, Hefei, China"},{"name":"Department of Microbiology, School of Basic Medical Sciences, Anhui Medical University, Hefei, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2021.04.002"},{"key":"e_1_3_3_3_2","unstructured":"World. 2023. 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