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In this study, we performed whole-genome sequencing of\n                      <jats:italic>P. multocida<\/jats:italic>\n                      isolates recovered from a range of human infections, from the mouths of cats, and from wounds on dogs. Together with publicly available\n                      <jats:italic>P. multocida<\/jats:italic>\n                      genome sequences, we performed phylogenetic and comparative genomic analyses. While isolates from cats and dogs were spread across the phylogenetic tree, human infections were caused almost exclusively by subsp.\n                      <jats:italic>septica<\/jats:italic>\n                      strains. Most of the human isolates were capsule type A and LPS type L1 and L3; however, some strains lacked a capsule biosynthesis locus, and some strains contained a novel LPS outer-core locus, distinct from the eight LPS loci that can currently be identified using an LPS multiplex PCR. In addition, the\n                      <jats:italic>P. multocida<\/jats:italic>\n                      strains isolated from human infections contained novel mobile genetic elements. We compiled a curated database of known\n                      <jats:italic>P. multocida<\/jats:italic>\n                      virulence factor and antibiotic resistance genes (PastyVRDB) allowing for detailed characterization of isolates. The majority of human\n                      <jats:italic>P. multocida<\/jats:italic>\n                      isolates encoded a reduced range of iron receptors and contained only one filamentous hemagglutinin gene. Finally, gene-trait analysis identified a putative L-fucose uptake and utilization pathway that was over-represented in subsp.\n                      <jats:italic>septica<\/jats:italic>\n                      strains and may represent a novel host predilection mechanism in this subspecies. Together, these analyses have identified pathogenic mechanisms likely important for\n                      <jats:italic>P. multocida<\/jats:italic>\n                      zoonotic infections.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        <jats:italic>Pasteurella multocida<\/jats:italic>\n                        can cause serious infections in humans, including skin and wound infections, pneumonia, peritonitis, meningitis, and bacteraemia. Cats and dogs are known vectors of human pasteurellosis, transmitting\n                        <jats:italic>P. multocida<\/jats:italic>\n                        via bite wounds or contact with animal saliva. The mechanisms that underpin\n                        <jats:italic>P. multocida<\/jats:italic>\n                        human predilection and pathogenesis are poorly understood. With increasing identification of antibiotic-resistant\n                        <jats:italic>P. multocida<\/jats:italic>\n                        strains, understanding these mechanisms is vital for developing novel treatments and control strategies to combat\n                        <jats:italic>P. multocida<\/jats:italic>\n                        human infection. Here, we show that a narrow range of\n                        <jats:italic>P. multocida<\/jats:italic>\n                        strains cause disease in humans, while cats and dogs, common vectors for zoonotic infections, can harbor a wide range of\n                        <jats:italic>P. multocida<\/jats:italic>\n                        strains. We also present a curated\n                        <jats:italic>P. multocida<\/jats:italic>\n                        -specific database, allowing quick and detailed characterization of newly sequenced\n                        <jats:italic>P. multocida<\/jats:italic>\n                        isolates.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.03805-23","type":"journal-article","created":{"date-parts":[[2024,3,1]],"date-time":"2024-03-01T09:01:12Z","timestamp":1709283672000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":34,"title":["Pathogenomic analysis and characterization of\n                    <i>Pasteurella multocida<\/i>\n                    strains recovered from human infections"],"prefix":"10.1128","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-3852-6518","authenticated-orcid":false,"given":"Thomas 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