{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T14:27:49Z","timestamp":1785421669569,"version":"3.56.0"},"reference-count":22,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T00:00:00Z","timestamp":1777939200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T00:00:00Z","timestamp":1777939200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["AI175342"],"award-info":[{"award-number":["AI175342"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2026,5,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Mycobacterium abscessus<\/jats:italic>\n                      is an emerging pathogen, extremely difficult to treat and with no FDA-approved drugs in clinic. Many antibiotics have limited activity against\n                      <jats:italic toggle=\"yes\">M. abscessus<\/jats:italic>\n                      , and their clinical breakpoints are not available. Post-antibiotic effect (PAE) is a determinant of antibiotic efficacy and could inform drug dosing regimens. Here, we evaluated and compared the duration of the PAE for seven antibiotics (amikacin, bedaquiline, cefoxitin, clarithromycin, imipenem, linezolid, and tigecycline), alone and in combination, toward\n                      <jats:italic toggle=\"yes\">M. abscessus<\/jats:italic>\n                      . The bioluminescence method used to evaluate the PAE was confirmed through conventional viable counting and offered a faster and easier approach to access PAE of individual antibiotics, their combinations, and could facilitate translation into clinical dosing. Clarithromycin and tigecycline induced a strong PAE (8.3\u201325.7 and 10.6\u201322 h, respectively), while amikacin (2\u201311 h), bedaquiline (1.8\u20134.5 h), imipenem (5 h), and linezolid (0.8\u20132 h) displayed moderate PAE activities. Cefoxitin showed no PAE. Inclusion of clarithromycin into four three-drug combinations used in the initial parenteral phase resulted in significant extension of the PAE in concordance with the better outcomes of these drug regimens. These results provide reference PAE values for\n                      <jats:italic toggle=\"yes\">M. abscessus<\/jats:italic>\n                      -active antibiotics and their combinations and offer a method to facilitate PAE testing\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      .\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Clinical infections with\n                        <jats:italic toggle=\"yes\">Mycobacterium abscessus<\/jats:italic>\n                        are on the rise globally, with no reliable cure options. Current treatment options rely on multidrug therapy including macrolide-based combinations and\/or amikacin; however, minimum inhibitory concentration (MIC) cutoffs for susceptibility and resistance have established clinical relevance only for macrolides and amikacin. No other\n                        <jats:italic toggle=\"yes\">M. abscessus<\/jats:italic>\n                        -active antibiotics have validated MIC cutoff points or available other parameters such as post-antibiotic effect (PAE) that could predict clinical response and dosing efficacy. Here, we report on the generation of a recombinant\n                        <jats:italic toggle=\"yes\">M. abscessus<\/jats:italic>\n                        reference strain expressing the bacterial\n                        <jats:italic toggle=\"yes\">lux<\/jats:italic>\n                        genes and its usage to monitor regrowth and quantify PAEs for current\n                        <jats:italic toggle=\"yes\">M. abscessus<\/jats:italic>\n                        antibiotics and their combinations used in clinic. These data provide reference numbers for current\n                        <jats:italic toggle=\"yes\">M. abscessus<\/jats:italic>\n                        antibiotics and offer a simplified approach to facilitate testing of new antibiotics and their combinations.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.03805-25","type":"journal-article","created":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T13:00:11Z","timestamp":1775566811000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of the post-antibiotic effect against\n                    <i>Mycobacterium abscessus<\/i>\n                    by a bioluminescence method"],"prefix":"10.1128","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4569-147X","authenticated-orcid":true,"given":"Isabelle","family":"Bonnet","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02r109517","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology and Immunology, Weill Cornell Medicine","place":["New York, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2179-671X","authenticated-orcid":true,"given":"Ruslana","family":"Bryk","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02r109517","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology and Immunology, Weill Cornell Medicine","place":["New York, 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Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.03805-25","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.03805-25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T13:06:25Z","timestamp":1779282385000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.03805-25"}},"subtitle":[],"editor":[{"given":"Aude 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