{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T16:52:10Z","timestamp":1776185530803,"version":"3.50.1"},"reference-count":20,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2024,5,2]],"date-time":"2024-05-02T00:00:00Z","timestamp":1714608000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,5,2]],"date-time":"2024-05-02T00:00:00Z","timestamp":1714608000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"crossref","award":["19-CE15-0012-01 (SUNLIVE)"],"award-info":[{"award-number":["19-CE15-0012-01 (SUNLIVE)"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002915","name":"Fondation pour la Recherche M\u00e9dicale","doi-asserted-by":"crossref","award":["Equipe FRM EQU202103012588"],"award-info":[{"award-number":["Equipe FRM EQU202103012588"]}],"id":[{"id":"10.13039\/501100002915","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2024,5,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              <jats:italic>Mycobacterium abscessus,<\/jats:italic>\n              an emerging pathogen responsible for severe pulmonary infections in cystic fibrosis patients, displays either a smooth (S) or a rough (R) morphotype. Infections with\n              <jats:italic>M. abscessus<\/jats:italic>\n              R are associated with increased pathogenicity in animal models and humans. While the S-to-R transition correlating with reduced glycopeptidolipid (GPL) production is well-documented, the recent screening of a transposon library revealed additional gene candidates located outside of the\n              <jats:italic>GPL<\/jats:italic>\n              locus involved in this transition. These genes include\n              <jats:italic>MAB_1470c<\/jats:italic>\n              , encoding the putative lipoprotein peptidase LpqM. However, experimental confirmation of the implication of this gene in the morphotype switch is lacking. Herein, we re-examined the role of\n              <jats:italic>MAB_1470c<\/jats:italic>\n              , and its homolog\n              <jats:italic>MAB_1466c<\/jats:italic>\n              , in colonial morphotype changes by generating unmarked deletion mutants in\n              <jats:italic>M. abscessus<\/jats:italic>\n              S. Our results indicate that the morphotype of these mutants stayed smooth in different media. Unexpectedly, the intracellular growth of \u0394\n              <jats:italic>MAB_1470c<\/jats:italic>\n              and \u0394\n              <jats:italic>MAB_1466c<\/jats:italic>\n              in THP-1 macrophages was significantly reduced as compared to the parental S strain, and these defects were rescued upon complementation with their corresponding genes. Strikingly, the intracellular survival defect was further exacerbated in a mutant lacking both\n              <jats:italic>MAB_1470c<\/jats:italic>\n              and\n              <jats:italic>MAB_1466c<\/jats:italic>\n              genes. This implies that, despite their primary sequence relatedness, the two proteins are not functionally redundant. Collectively, this suggests that these two LpqM-related lipoproteins are unlikely to be involved in the S-to-R transition but are key players for intramacrophage survival of\n              <jats:italic>M. abscessus<\/jats:italic>\n              .\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              <jats:italic>Mycobacterium abscessus<\/jats:italic>\n              causes persistent infections in patients with underlying pulmonary diseases, resulting in progressive lung function deterioration. The rough (R) morphotype is well-established as associated with chronic and more aggressive infections in patients. In this study, we individually and simultaneously deleted the\n              <jats:italic>MAB_1470c<\/jats:italic>\n              and\n              <jats:italic>MAB_1466c<\/jats:italic>\n              genes in\n              <jats:italic>M. abscessus<\/jats:italic>\n              S, without observing changes in colony morphotypes. However, these mutants exhibited a severe impairment in their ability to survive within human macrophages, highlighting the critical role of these two lipoproteins in\n              <jats:italic>M. abscessus<\/jats:italic>\n              virulence.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.03837-23","type":"journal-article","created":{"date-parts":[[2024,4,15]],"date-time":"2024-04-15T13:00:16Z","timestamp":1713186016000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["Loss of LpqM proteins in\n            <i>Mycobacterium abscessus<\/i>\n            is associated with impaired intramacrophage survival"],"prefix":"10.1128","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0021-276X","authenticated-orcid":false,"given":"Yves-Marie","family":"Boudehen","sequence":"first","affiliation":[{"name":"Centre National de la Recherche Scientifique UMR 9004, Institut de Recherche en Infectiologie de Montpellier (IRIM), Universit\u00e9 de Montpellier, Montpellier, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wassim","family":"Daher","sequence":"additional","affiliation":[{"name":"Centre National de la Recherche Scientifique UMR 9004, Institut de Recherche en Infectiologie de Montpellier (IRIM), Universit\u00e9 de Montpellier, Montpellier, France"},{"name":"INSERM, IRIM, Montpellier, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2636-3734","authenticated-orcid":false,"given":"Fran\u00e7oise","family":"Roquet-Baneres","sequence":"additional","affiliation":[{"name":"Centre National de la Recherche Scientifique UMR 9004, Institut de Recherche en Infectiologie de Montpellier (IRIM), Universit\u00e9 de Montpellier, Montpellier, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6604-4458","authenticated-orcid":true,"given":"Laurent","family":"Kremer","sequence":"additional","affiliation":[{"name":"Centre National de la Recherche Scientifique UMR 9004, Institut de Recherche en Infectiologie de Montpellier (IRIM), Universit\u00e9 de Montpellier, Montpellier, France"},{"name":"INSERM, IRIM, Montpellier, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-020-0331-1"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1098\/rsob.160185"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.28625-0"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12387"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02592-06"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01478-08"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiad007"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02322-10"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1321390111"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2010.01565.x"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.03431-20"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2022.03.008"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSystems.00976-20"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00024-09"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2006\/000216-0"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01316-18"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.01145"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1021\/acsinfecdis.0c00490"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsre.2004.06.002"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA119.008781"}],"container-title":["Microbiology 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