{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T15:50:55Z","timestamp":1786117855497,"version":"build-2736575974"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2026,8,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Despite its dominance as a commensal,\n                      <jats:italic toggle=\"yes\">Staphylococcus lugdunensis<\/jats:italic>\n                      (SLU) is a particularly virulent coagulase-negative\n                      <jats:italic toggle=\"yes\">Staphylococcus<\/jats:italic>\n                      that is predominantly associated with community-acquired soft-tissue and skin infections. The aim of our study was to identify genotypic and phenotypic traits associated with the clinical presentations of SLU-associated infections, such as prosthetic joint infections, infective endocarditis (IE), and skin and soft tissue infections. Between January 2001 and December 2016, 73 pathogenic (including 18 strains responsible for IE) and 3 nonpathogenic SLU strains were retrospectively collected from nine French hospitals. All the strains belonged to six clonal complexes (CC1 to CC6) and 12 sequence types (STs). Interestingly, the capacity of the IE-associated strains to bind the von Willebrand factor was greater than that of the other strains, regardless of the CC. Biofilm formation was significantly increased for the most common CCs involved in infections (CC1, CC2, and CC3) and for IE-associated strains. The ability of SLU to inhibit other pathogens varied depending on the CCs caused by specific mutations within the\n                      <jats:italic toggle=\"yes\">lug<\/jats:italic>\n                      operon that resulted in truncated protein expression. Genetic variants from genome virulence genes such as\n                      <jats:italic toggle=\"yes\">agrC<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">atIL<\/jats:italic>\n                      also vary according to STs. The\n                      <jats:italic toggle=\"yes\">Galleria mellonella<\/jats:italic>\n                      infection model revealed that CC4 was more virulent than the other CCs. On the basis of a large sequenced collection of SLU strains, we found that important phenotypic variation occurred among clinical strains and seemed to be related to some type of infection.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Staphylococcus lugdunensis<\/jats:italic>\n                      is a coagulase-negative\n                      <jats:italic toggle=\"yes\">Staphylococcus<\/jats:italic>\n                      recognized for its virulence in human clinical infections. However, few studies have focused on investigating the phenotypic and genotypic aspects, as well as the population characteristics, of clinical strains involved in severe invasive infections such as endocarditis or skin and soft tissue infections, or of strains associated with so-called cutaneous carriage. Here, we compared different virulence gene variants in terms of populational aspects and also highlighted important phenotypic trait changes according to the clinical presentation and site of isolation of the strains.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.03856-25","type":"journal-article","created":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T13:01:22Z","timestamp":1782306082000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Phenotypic and genotypic characterization of clinical\n                    <i>Staphylococcus lugdunensis<\/i>\n                    isolates: a French retrospective cohort 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