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Mechanistically, ch-MYC-AS1 binds to annexin A2 (ANXA2) and impedes its nuclear translocation, preventing its collaboration with c-Myc to promote glycolysis. This dual inhibition suppresses c-Myc-driven metabolic reprogramming essential for viral proliferation. Our findings reveal ch-MYC-AS1 as a key suppressor of retroviral replication through coordinated disruption of c-Myc\/ANXA2 signaling, providing a potential therapeutic strategy for antiviral and anticancer drug development.<\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>Avian leukosis virus subgroup J (ALV-J) is an oncogenic retrovirus that causes tumors and immunosuppression in chickens, leading to significant economic losses in poultry industries. This study identifies a host-derived long noncoding RNA (lncRNA), ch-MYC-AS1, which suppresses ALV-J replication by disrupting the ANXA2-c-Myc signaling axis. These findings unveil a novel layer of antiviral defense mediated by an epigenetically regulated lncRNA and highlight a potential RNA-based strategy to combat retroviral infections. Moreover, targeting the ANXA2-c-Myc interaction may offer therapeutic insights for controlling ALV-J and other MYC-driven diseases.<\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.03880-25","type":"journal-article","created":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T12:00:56Z","timestamp":1774872056000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["lncRNA ch-MYC-AS1 restricts ALV-J replication by disrupting the ANXA2-C-Myc oncogenic axis"],"prefix":"10.1128","volume":"14","author":[{"given":"Suyu","family":"Fan","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03tqb8s11","id-type":"ROR","asserted-by":"publisher"}],"name":"Jiangsu Key Laboratory for Animal Genetic, Breeding and Molecular Design, College of Animal Science and Technology, Yangzhou University","place":["Yangzhou, 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