{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T13:55:46Z","timestamp":1781704546095,"version":"3.54.5"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T00:00:00Z","timestamp":1780358400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T00:00:00Z","timestamp":1780358400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Sichuan Provincial Major Science and Technology Project","award":["2021ZDZX0010-3"],"award-info":[{"award-number":["2021ZDZX0010-3"]}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2024YFD1800500, 2024YFD1800102"],"award-info":[{"award-number":["2024YFD1800500, 2024YFD1800102"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Pig Technology Innovation Center","award":["NCTIP-MY23006"],"award-info":[{"award-number":["NCTIP-MY23006"]}]},{"name":"Sichuan Science and Technology Program Projects","award":["2023YFN0021, 2022YFN0007"],"award-info":[{"award-number":["2023YFN0021, 2022YFN0007"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2026,6,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>Pseudorabies virus (PRV) causes rapidly progressive and often fatal encephalitis in both natural and non-natural hosts, making the development of effective preventive strategies critically important. Among current vaccine candidates, the triple-gene-deleted PRV strain (PRV-delgE\/gI\/TK) has been widely studied; however, its protective effects against brain injury, particularly blood\u2013brain barrier (BBB) damage, remain poorly characterized. To address this gap, we established an intracerebral challenge model in C57BL\/6J mice to directly evaluate central nervous system (CNS) outcomes following wild-type PRV infection. Wild-type PRV challenge resulted in severe neurological deficits, uniform mortality, extensive neuronal degeneration, pronounced BBB disruption, downregulation of tight junction proteins, strong induction of MMP-2\/9, and a robust inflammatory cytokine surge. In contrast, mice immunized with PRV-delgE\/gI\/TK exhibited only mild and transient symptoms, rapid functional recovery, markedly reduced viral replication, and complete viral clearance by 14 days. Immunization preserved BBB integrity, maintained ZO-1 and occludin expression, prevented MMP activation, and substantially blunted neuroinflammatory responses. Collectively, these results show that PRV-delgE\/gI\/TK immunization effectively protects against wild-type PRV-induced CNS injury in C57BL\/6J mice, as evidenced by reduced viral burden, preserved BBB integrity, and attenuated neuroinflammatory responses, and provide new insight into the neuroprotective effects of attenuated PRV vaccination.<\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Pseudorabies virus (PRV) can cause severe and often fatal encephalitis, but the central nervous system (CNS)-protective effects of attenuated PRV vaccines remain insufficiently characterized, particularly with respect to blood\u2013brain barrier (BBB) injury. Using a C57BL\/6J intracerebral challenge model, this study shows that immunization with the triple-gene-deleted PRV-delgE\/gI\/TK strain markedly reduces viral replication, preserves BBB integrity, and attenuates neuroinflammatory responses after wild-type PRV challenge. These findings provide\n                        <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                        evidence that attenuated PRV immunization can protect against PRV-induced CNS injury and offer important insights for improving prevention strategies against neurotropic PRV infection.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.03930-25","type":"journal-article","created":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T13:00:39Z","timestamp":1778072439000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["PRV-delgE\/gI\/TK immunization preserves blood\u2013brain barrier integrity and limits CNS injury following intracerebral PRV challenge"],"prefix":"10.1128","volume":"14","author":[{"given":"Tong","family":"Xu","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Sichuan 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