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In this study, we demonstrated the anti-fungal efficacy of the AH derivative D13 [4-bromo-\n              <jats:italic>N<\/jats:italic>\n              \u2032-(3,5-dibromo-2-hydroxybenzylidene)-benzohydrazide] against both planktonic cells and biofilms formed by\n              <jats:italic>Sporothrix brasiliensis<\/jats:italic>\n              . In a clinical study, the effect of D13 was then tested in combination with itraconazole (ITC), with or without potassium iodide, in 10 cats with sporotrichosis refractory to the treatment of standard of care with ITC. Improvement or total clinical cure was achieved in five cases after 12 weeks of treatment. Minimal abnormal laboratory findings, e.g., elevation of alanine aminotransferase, were observed in four cats during the combination treatment and returned to normal level within a week after the treatment was ended. Although highly encouraging, a larger and randomized controlled study is required to evaluate the effectiveness and the safety of this new and exciting drug combination using ITC and D13 for the treatment of feline sporotrichosis.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>This paper reports the first veterinary clinical study of an acylhydrazone anti-fungal (D13) combined with itraconazole against a dimorphic fungal infection, sporotrichosis, which is highly endemic in South America in animals and humans. Overall, the results show that the combination treatment was efficacious in ~50% of the infected animals. In addition, D13 was well tolerated during the course of the study. Thus, these results warrant the continuation of the research and development of this new class of anti-fungals.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.03967-23","type":"journal-article","created":{"date-parts":[[2024,4,22]],"date-time":"2024-04-22T13:05:04Z","timestamp":1713791104000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Combination therapy of itraconazole and an acylhydrazone derivative (D13) for the treatment of sporotrichosis in cats"],"prefix":"10.1128","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8428-7616","authenticated-orcid":false,"given":"Isabella","family":"Dib Ferreira Gremi\u00e3o","sequence":"first","affiliation":[{"name":"Laboratory of Clinical Research on Dermatozoonoses in Domestic Animals, Evandro Chagas National Institute of Infectious Diseases, Rio de Janeiro, 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Pereira","family":"Borba-Santos","sequence":"additional","affiliation":[{"name":"Laboratory of Fungal Cell Biology, Carlos Chagas Filho Institute of Biophysics, Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandra Lifsitch","family":"Vi\u00e7osa","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Pharmacotechnics, Institute of Drug Technology \u2013 Farmanguinhos, Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashna","family":"Garg","sequence":"additional","affiliation":[{"name":"Institute of Chemical Biology and Drug Discovery, Stony Brook University, Stony Brook, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Krupanandan","family":"Haranahalli","sequence":"additional","affiliation":[{"name":"Institute of Chemical Biology and Drug Discovery, Stony Brook University, Stony Brook, New York, 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