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The emergence of multidrug-resistant (MDR) APEC poses zoonotic risks through horizontal transfer of antimicrobial resistance (AMR) genes. Bacteriophage therapy emerges as a safe alternative to antibiotherapy; however, comprehensive characterization of phages targeting MDR-APEC from diverse geographical regions remains limited. We isolated five lytic bacteriophages from poultry fecal samples collected from five Indian states and characterized them through morphological analysis, physiological stability testing, whole-genome sequencing, and\n                      <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                      efficacy assessment. Host range was determined against APEC isolates, and therapeutic potential was validated in the\n                      <jats:italic toggle=\"yes\">Galleria mellonella<\/jats:italic>\n                      infection model. All phages showed Myovirus-like morphology and stability across physiologically relevant temperatures (up to 55\u00b0C\u201370\u00b0C) and pH conditions (3\u201311). Phages were classified as\n                      <jats:italic toggle=\"yes\">Escherichia<\/jats:italic>\n                      phage vB_EcoM_fRPOT1, vB_EcoM_fDMYT1, vB_EcoM_fBSZT1, vB_EcoM_fUAMT1, and vB_EcoM_fPKPT2. Their genome size ranges from 170 to 356 kb, belonging to three distinct genera:\n                      <jats:italic toggle=\"yes\">Dhakavirus<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">Gaprivervirus<\/jats:italic>\n                      , and\n                      <jats:italic toggle=\"yes\">Asteriusvirus<\/jats:italic>\n                      . Genomic analysis confirmed the absence of antimicrobial resistance, virulence, toxin, or lysogeny genes. Fifty-one APEC strains were isolated, of which 23 (45.1%) were MDR. Individual phages lysed 37%\u201351% of tested APEC and 17%\u201339% of MDR strains. Three phages (fBSZT1, fUAMT1, and fPKPT2) significantly improved larval survival to 60%\u201380% at an MOI of 10 in\n                      <jats:italic toggle=\"yes\">G. mellonella<\/jats:italic>\n                      infection models compared to the untreated control. This study establishes a well-characterized phage bank targeting MDR-APEC strains, providing a foundation for developing phage-based interventions to reduce antibiotic dependency and mitigate AMR transmission risks under the One Health framework.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>The overuse of antibiotics in poultry farming has created a crisis. The multidrug-resistant (MDR) bacteria threaten both animal health and human safety through the food chain. When antibiotics fail, farmers face devastating losses, and resistant bacteria can transfer to humans through consumption or environmental contamination. Bacteriophages offer a practical solution as they kill target bacteria without harming beneficial microbes or leaving chemical residues. Our comprehensive characterization confirms that these five phages are safe and effective as they lack any resistance or toxin genes and rescue 60%\u201380% of infected larvae. This represents a characterized phage bank targeting the specific resistant strains in Indian poultry. By providing a validated alternative to antibiotics, this work supports sustainable food production while reducing the spread of antimicrobial resistance from farms to humans.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.03979-25","type":"journal-article","created":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T04:00:17Z","timestamp":1784520017000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Genomic characterization and therapeutic potential of five broad-spectrum lytic bacteriophages against multidrug-resistant avian pathogenic\n                    <i>Escherichia coli<\/i>\n                    (APEC)"],"prefix":"10.1128","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2506-4719","authenticated-orcid":false,"given":"Tushar","family":"Midha","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02kknsa06","id-type":"ROR","asserted-by":"publisher"}],"name":"Gastrointestinal Disease Laboratory, Department of Microbiology, School of Basic Sciences, Central University of Punjab","place":["Bathinda, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"family":"Vishakha","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02kknsa06","id-type":"ROR","asserted-by":"publisher"}],"name":"Gastrointestinal Disease Laboratory, Department of Microbiology, School of Basic Sciences, Central University of Punjab","place":["Bathinda, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6065-559X","authenticated-orcid":true,"given":"Somesh","family":"baranwal","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02kknsa06","id-type":"ROR","asserted-by":"publisher"}],"name":"Gastrointestinal Disease Laboratory, Department of Microbiology, School of Basic Sciences, Central University of Punjab","place":["Bathinda, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.psj.2025.105038"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.psj.2024.104359"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2014.00282"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2024.106843"},{"key":"e_1_3_4_6_2","volume-title":"WHO bacterial priority pathogens list, 2024: bacterial pathogens of public health importance, to guide research, development, and strategies to prevent and control antimicrobial resistance","author":"World Health Organization","year":"2024","unstructured":"World Health Organization. 2024. 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