{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T15:39:36Z","timestamp":1792510776817,"version":"4.5.0"},"reference-count":59,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31701831"],"award-info":[{"award-number":["31701831"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2026,10,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Copper serves as an indispensable trace element in living organisms, playing pivotal roles in fundamental physiological processes including enzyme assembly, antioxidant defense, and metabolic regulation. However, excess copper can induce oxidative damage. MerR-family regulator CueR acts as the core mediator of copper homeostasis in prokaryotes, but its mechanism of action in\n                      <jats:italic toggle=\"yes\">P. chlororaphis<\/jats:italic>\n                      zm-1, a plant biocontrol bacterium that inhibits peanut stem rot, remains unclear. In this study, we investigated the regulatory function of CueR using molecular techniques including gene deletion, electrophoretic mobility shift assay, and quantitative real-time PCR. The results demonstrated that the transcriptional regulator gene\n                      <jats:italic toggle=\"yes\">cueR<\/jats:italic>\n                      and the P\n                      <jats:sub>1B<\/jats:sub>\n                      -type ATPase gene\n                      <jats:italic toggle=\"yes\">cueA<\/jats:italic>\n                      (involved in copper efflux) form the\n                      <jats:italic toggle=\"yes\">cueRA<\/jats:italic>\n                      operon in strain zm-1, with a 4-bp overlap and co-transcriptional feature. CueR directly binds to the promoter region and positively activates\n                      <jats:italic toggle=\"yes\">cueRA<\/jats:italic>\n                      expression upon copper ion induction, thereby driving CueA-mediated efflux of excess copper ions. The deletion of\n                      <jats:italic toggle=\"yes\">cueR<\/jats:italic>\n                      or\n                      <jats:italic toggle=\"yes\">cueA<\/jats:italic>\n                      significantly reduced copper tolerance, increased intracellular copper content, and impaired peanut rhizosphere colonization ability of the strain, while these phenotypes were restored in the complementation strains. This study deciphers the regulatory mechanism of the\n                      <jats:italic toggle=\"yes\">cueRA<\/jats:italic>\n                      operon in\n                      <jats:italic toggle=\"yes\">P. chlororaphis<\/jats:italic>\n                      and establishes a correlation analysis between this mechanism and the rhizosphere colonization capacity of the strain. The findings provide a molecular basis for understanding the diversity of copper homeostasis regulation in\n                      <jats:italic toggle=\"yes\">Pseudomonas<\/jats:italic>\n                      and optimizing the biocontrol application of this bacterium.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        The CueR-regulated copper efflux system is essential for bacterial survival under copper overload conditions. While this system has been extensively characterized in human pathogens, its function in plant-beneficial bacteria remains poorly understood. Here, we investigate the agricultural biocontrol strain\n                        <jats:italic toggle=\"yes\">P. chlororaphis<\/jats:italic>\n                        zm-1 and delineate the regulatory mechanism of CueR through molecular approaches, leading to the identification of the\n                        <jats:italic toggle=\"yes\">cueRA<\/jats:italic>\n                        operon. Furthermore, we establish a functional link between CueR and rhizosphere colonization, thereby providing a framework for understanding CueR-mediated regulation in plant-associated bacteria.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.03981-25","type":"journal-article","created":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T13:03:30Z","timestamp":1789650210000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["The transcriptional regulator CueR positively regulates the expression of the\n                    <i>cueRA<\/i>\n                    operon to maintain copper homeostasis in\n                    <i>Pseudomonas chlororaphis<\/i>\n                    zm-1"],"prefix":"10.1128","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-2570-2382","authenticated-orcid":true,"given":"Zhongkang","family":"Wang","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/003xyzq10","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Life Sciences, Henan University","place":["Kaifeng, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Li","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/003xyzq10","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Life Sciences, Henan University","place":["Kaifeng, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenming","family":"Lei","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/003xyzq10","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Life Sciences, Henan University","place":["Kaifeng, 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