{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T12:04:06Z","timestamp":1792757046019,"version":"4.5.0"},"reference-count":77,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2024,6,4]],"date-time":"2024-06-04T00:00:00Z","timestamp":1717459200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,6,4]],"date-time":"2024-06-04T00:00:00Z","timestamp":1717459200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2024,6,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              A collection of 161\n              <jats:italic>Ralstonia<\/jats:italic>\n              isolates, including 90 isolates from persons with cystic fibrosis, 27 isolates from other human clinical samples, 8 isolates from the hospital environment, 7 isolates from industrial samples, and 19 environmental isolates, was subjected to matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry (MALDI-TOF MS) identification and yielded confident species level identification scores for only 62 (39%) of the isolates, including four that proved misidentified subsequently. Whole-genome sequence analysis of 32 representative isolates for which no confident MALDI-TOF MS species level identification was obtained revealed the presence of seven novel\n              <jats:italic>Ralstonia<\/jats:italic>\n              species, including three and four that were isolated from cystic fibrosis or other human clinical samples, respectively, and provided the basis for updating an\n              <jats:italic>in-house<\/jats:italic>\n              MALDI-TOF MS database. A reanalysis of all mass spectra with the updated MALDI-TOF MS database increased the percentage of isolates with confident species level identification up to 77%. The antimicrobial susceptibility of 30 isolates mainly representing novel human clinical and environmental\n              <jats:italic>Ralstonia<\/jats:italic>\n              species was tested toward 17 antimicrobial agents and demonstrated that the novel\n              <jats:italic>Ralstonia<\/jats:italic>\n              species were generally multi-resistant, yet susceptible to trimethoprim\/sulfamethoxazole, ciprofloxacin, and tigecycline. An analysis of genomic antimicrobial resistance genes in 32 novel and publicly available genome sequences revealed broadly distributed beta-lactam resistance determinants.\n            <\/jats:p>\n            <jats:sec>\n              <jats:title>IMPORTANCE<\/jats:title>\n              <jats:p>\n                The present study demonstrated that a commercial matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry identification database can be tailored to improve the identification of\n                <jats:italic>Ralstonia<\/jats:italic>\n                species. It also revealed the presence of seven novel\n                <jats:italic>Ralstonia<\/jats:italic>\n                species, including three and four that were isolated from cystic fibrosis or other human clinical samples, respectively. An analysis of minimum inhibitory concentration values demonstrated that the novel\n                <jats:italic>Ralstonia<\/jats:italic>\n                species were generally multi-resistant but susceptible to trimethoprim\/sulfamethoxazole, ciprofloxacin, and tigecycline.\n              <\/jats:p>\n            <\/jats:sec>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.04021-23","type":"journal-article","created":{"date-parts":[[2024,4,25]],"date-time":"2024-04-25T13:00:51Z","timestamp":1714050051000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":18,"title":["Novel\n            <i>Ralstonia<\/i>\n            species from human infections: improved matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry-based identification and analysis of antimicrobial resistance patterns"],"prefix":"10.1128","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6953-7047","authenticated-orcid":true,"given":"Stephanie","family":"Steyaert","sequence":"first","affiliation":[{"name":"Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, Gent, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1891-4869","authenticated-orcid":true,"given":"Charlotte","family":"Peeters","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, Gent, Belgium"},{"name":"National Reference Center for Burkholderia cepacia complex, La Plata, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4503-4063","authenticated-orcid":false,"given":"Anneleen D.","family":"Wieme","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, Gent, Belgium"},{"name":"BCCM\/LMG Bacteria Collection, Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Astrid","family":"Muyldermans","sequence":"additional","affiliation":[{"name":"National Reference Center for Burkholderia cepacia complex, La Plata, Belgium"},{"name":"Department of Microbiology and Infection Control, Vrije Universiteit Brussel (VUB), Universitair Ziekenhuis Brussel (UZ Brussel), Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristof","family":"Vandoorslaer","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infection Control, Vrije Universiteit Brussel (VUB), Universitair Ziekenhuis Brussel (UZ Brussel), Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theodore","family":"Spilker","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Michigan Medical School, Ann Arbor, Michigan, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6217-1695","authenticated-orcid":true,"given":"Ingrid","family":"Wybo","sequence":"additional","affiliation":[{"name":"National Reference Center for Burkholderia cepacia complex, La Plata, Belgium"},{"name":"Department of Microbiology and Infection Control, Vrije Universiteit Brussel (VUB), Universitair Ziekenhuis Brussel (UZ Brussel), Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denis","family":"Pi\u00e9rard","sequence":"additional","affiliation":[{"name":"National Reference Center for Burkholderia cepacia complex, La Plata, Belgium"},{"name":"Department of Microbiology and Infection Control, Vrije Universiteit Brussel (VUB), Universitair Ziekenhuis Brussel (UZ Brussel), Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4033-7794","authenticated-orcid":true,"given":"John J.","family":"LiPuma","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Michigan Medical School, Ann Arbor, Michigan, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5581-7937","authenticated-orcid":false,"given":"Peter","family":"Vandamme","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, Gent, Belgium"},{"name":"National Reference Center for Burkholderia cepacia complex, La Plata, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.6.2062-2069.2002"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0807.010472"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.rmcr.2016.11.014"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00068-09"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.prrv.2016.09.005"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.02555-0"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-51-2-547"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-23-1-15"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.1995.tb03275.x"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2023.1135872"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0723-2020(11)80178-5"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.066712-0"},{"key":"e_1_3_4_14_2","volume-title":"Bull div Veg Physiol Pathol US Dep Agric 12","author":"Smith E","year":"1896","unstructured":"Smith E. 1896. A bacterial disease of the tomato, eggplant, and Irish potato (Bacillus solanacearum n. sp.). In Bull div Veg Physiol Pathol US Dep Agric 12"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.02754-0"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.005622"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2023.1179087"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268817001728"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1097\/MCP.0000000000000517"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-013-1975-9"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00569-08"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01618-13"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcf.2011.09.001"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10482-021-01637-0"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.7.3463-3466.2005"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSystems.00437-19"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-004-1812-2"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2011.06.004"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty560"},{"key":"e_1_3_4_31_2","unstructured":"Seemann T. 2020. Shovill. https:\/\/github.com\/tseemann\/abricate."},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1089\/cmb.2012.0021"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btt086"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.186072.114"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu153"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab902"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-10210-3"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-14-60"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-07641-9"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btac672"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab776"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2011.189"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1139\/gen-2015-0175"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab301"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac920"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab1107"},{"key":"e_1_3_4_48_2","doi-asserted-by":"publisher","DOI":"10.1080\/14756366.2017.1344235"},{"key":"e_1_3_4_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.66.1.24-26.1953"},{"key":"e_1_3_4_50_2","volume-title":"Biochemical tests for identification of medical bacteria","author":"MacFaddin J","year":"2000","unstructured":"MacFaddin J. 2000. Biochemical tests for identification of medical bacteria. Williams and Wilkins."},{"key":"e_1_3_4_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.syapm.2022.126318"},{"key":"e_1_3_4_52_2","doi-asserted-by":"publisher","DOI":"10.4056\/sigs.531120"},{"key":"e_1_3_4_53_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10482-017-0844-4"},{"key":"e_1_3_4_54_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.45656-0"},{"key":"e_1_3_4_55_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.12.5008-5011.1998"},{"key":"e_1_3_4_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2020.105889"},{"key":"e_1_3_4_57_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-011-5656-1"},{"key":"e_1_3_4_58_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.1.1.61-64.1975"},{"key":"e_1_3_4_59_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-114-1-137"},{"key":"e_1_3_4_60_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-120-2-439"},{"key":"e_1_3_4_61_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(72)90981-6"},{"key":"e_1_3_4_62_2","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-9-4-493"},{"key":"e_1_3_4_63_2","doi-asserted-by":"publisher","DOI":"10.1017\/s0950268800047725"},{"key":"e_1_3_4_64_2","doi-asserted-by":"publisher","DOI":"10.1016\/0195-6701(90)90049-t"},{"key":"e_1_3_4_65_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.21.2.278-279.1985"},{"key":"e_1_3_4_66_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.32.4.1132-1133.1994"},{"key":"e_1_3_4_67_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-45-2-274"},{"key":"e_1_3_4_68_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-6-66"},{"key":"e_1_3_4_69_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01310-05"},{"key":"e_1_3_4_70_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1999.tb13593.x"},{"key":"e_1_3_4_71_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01069-21"},{"key":"e_1_3_4_72_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-019-3985-4"},{"key":"e_1_3_4_73_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.054759-0"},{"key":"e_1_3_4_74_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2004.10.002"},{"key":"e_1_3_4_75_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12302-020-00357-y"},{"key":"e_1_3_4_76_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.drup.2010.08.003"},{"key":"e_1_3_4_77_2","doi-asserted-by":"publisher","DOI":"10.1086\/521367"},{"key":"e_1_3_4_78_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.76.010307.145803"},{"key":"e_1_3_4_79_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.11.4217-4225.2004"},{"key":"e_1_3_4_80_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.8.2201-2204.2000"}],"container-title":["Microbiology Spectrum"],"original-title":[],"contributor":[{"given":"Mikel Joseba","family":"Urrutikoetxea","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"reviewer"}]},{"given":"Alejandra","family":"Bosch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"reviewer"}]}],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.04021-23","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.04021-23","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,4]],"date-time":"2024-06-04T14:16:14Z","timestamp":1717510574000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.04021-23"}},"subtitle":[],"editor":[{"given":"Silvia T.","family":"Cardona","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2024,6,4]]},"references-count":77,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2024,6,4]]}},"alternative-id":["10.1128\/spectrum.04021-23"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.04021-23","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,6,4]]},"assertion":[{"value":"2023-11-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-03-13","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e04021-23"}}