{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T00:49:16Z","timestamp":1782175756980,"version":"3.54.5"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2023,4,13]],"date-time":"2023-04-13T00:00:00Z","timestamp":1681344000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,4,13]],"date-time":"2023-04-13T00:00:00Z","timestamp":1681344000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100018555","name":"\u8d35\u5dde\u7701\u79d1\u6280\u5385 | Science and Technology Program of Guizhou Province","doi-asserted-by":"publisher","award":["Qian Ke He [2020]4Y184"],"award-info":[{"award-number":["Qian Ke He [2020]4Y184"]}],"id":[{"id":"10.13039\/501100018555","id-type":"DOI","asserted-by":"publisher"}]},{"name":"The Program of Science and Technology of Guizhou Provincial Health Commission","award":["gzwjkj2022-1-497"],"award-info":[{"award-number":["gzwjkj2022-1-497"]}]},{"name":"The Scientific and Technological in Guiyang City","award":["Zhu Ke He [2020]-10-5"],"award-info":[{"award-number":["Zhu Ke He [2020]-10-5"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2023,4,13]]},"abstract":"<jats:p>Monkeypox (MPX), a reemerging zoonotic disease caused by monkeypox virus (MPXV), causes a smallpox-like disease in humans. Early diagnosis is critical to prevent MPX epidemics. Here, CRISPR\/Cas12b was integrated with LAMP amplification to devise a novel CRISPR-MPXV approach to achieve highly specific, ultrasensitive, rapid, and visual identification of MPXV Congo Basin and West African strains.<\/jats:p>","DOI":"10.1128\/spectrum.04035-22","type":"journal-article","created":{"date-parts":[[2023,2,22]],"date-time":"2023-02-22T14:00:13Z","timestamp":1677074413000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":18,"title":["Ultrasensitive and Specific Identification of Monkeypox Virus Congo Basin and West African Strains Using a CRISPR\/Cas12b-Based Platform"],"prefix":"10.1128","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1583-3916","authenticated-orcid":true,"given":"Xu","family":"Chen","sequence":"first","affiliation":[{"name":"The Second Clinical College, Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou, People\u2019s Republic of China"},{"name":"Clinical Medical Laboratory of the Second Affiliated Hospital, Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Quality Control, Guizhou Provincial Center for Clinical Laboratory, Guiyang, Guizhou, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinggui","family":"Yang","sequence":"additional","affiliation":[{"name":"Guizhou Provincial Centre for Disease Control and Prevention, Guiyang, Guizhou, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanfang","family":"Shi","sequence":"additional","affiliation":[{"name":"The Second Clinical College, Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyan","family":"Zeng","sequence":"additional","affiliation":[{"name":"The Second Clinical College, Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou, People\u2019s Republic of China"},{"name":"Clinical Medical Laboratory of the Second Affiliated Hospital, Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junfei","family":"Huang","sequence":"additional","affiliation":[{"name":"Guizhou Provincial Centre for Disease Control and Prevention, Guiyang, Guizhou, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Wang","sequence":"additional","affiliation":[{"name":"Experimental Research Center, Capital Institute of Pediatrics, Beijing, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shijun","family":"Li","sequence":"additional","affiliation":[{"name":"Guizhou Provincial Centre for Disease Control and Prevention, Guiyang, Guizhou, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2704.203569"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.idc.2019.03.001"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.add2701"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.amsu.2022.103975"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2022.27.22.2200422"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2022.12513"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s40808-021-01313-2"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virs.2022.07.006"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.3390\/v12111257"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2604.191164"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(22)02075-X"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.7759\/cureus.26756"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-019-0131-5"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.13925"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaq0179"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aas8836"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1042\/BST20190662"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2021.02.018"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2020.112430"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aar6245"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41596-019-0210-2"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1021\/acssynbio.9b00209"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00604-019-3822-x"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.7150\/thno.56471"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.0c10035"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2015.05.050"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.21494"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2019.03.015"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2010.07.012"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2021.12.002"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2021.04.007"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.synbio.2018.09.004"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-021-02741-5"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.02714-22"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.12.e63"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2017.03.016"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1111\/1348-0421.12734"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.7150\/thno.44298"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2017.09.001"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.04035-22","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.04035-22","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,13]],"date-time":"2023-04-13T13:21:03Z","timestamp":1681392063000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.04035-22"}},"subtitle":[],"editor":[{"given":"Yun Young","family":"Go","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2023,4,13]]},"references-count":39,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2023,4,13]]}},"alternative-id":["10.1128\/spectrum.04035-22"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.04035-22","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,13]]},"assertion":[{"value":"2022-10-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-01-29","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-02-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e04035-22"}}