{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T12:28:54Z","timestamp":1785414534117,"version":"3.56.0"},"reference-count":34,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T00:00:00Z","timestamp":1780358400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T00:00:00Z","timestamp":1780358400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2026,6,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Multidrug-resistant\n                      <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                      has been classified as a pathogen of high priority. However, limited or mixed-quality studies reported multidrug-resistant\n                      <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                      strains from pediatric patients. The study aimed to investigate the prevalence, serotypes, antimicrobial characteristics, and plasmid profiles of clinical\n                      <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                      strains isolated from pediatric patients. For that, we collected 46\n                      <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                      isolates from children\u2019s fecal samples in multiple hospitals. Antimicrobial susceptibility testing, conjugation assay, short-read and long-read sequencing, and bioinformatics analysis were performed. Our data revealed that the top three serotypes of the 46 isolated strains are\n                      <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                      I 4,[5],12:i:-, Typhimurium, and Enteritidis, with prevalence at 34.78% (16\/46), 23.91% (11\/46), and 17.39% (8\/46), respectively. Most of these strains clustered into ST34, ST19, and ST11. Antimicrobial susceptibility testing analysis suggested that more than 80% of these\n                      <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                      isolates belong to multidrug-resistant strains, all of them showed resistance to ampicillin, ampicillin-sulbactam, and trimethoprim-sulfamethoxazole, and all strains were susceptible to meropenem, with about 17.39% of them having minimum inhibitory concentrations \u22654 \u00b5g\/mL to imipenem. Moreover, conjugation assay and long-read sequencing analysis on the plasmids harbored in 12 of the 46\n                      <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                      strains consistently showed that none of them has intact conjugative transfer genes, indicating\n                      <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                      strains from pediatric patients have different antimicrobial resistance levels and impaired plasmid transferability due to loss of part or whole\n                      <jats:italic toggle=\"yes\">tra<\/jats:italic>\n                      genes.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                        , one of the world\u2019s top prevalent foodborne pathogens, has gained resistance to fluoroquinolone and many other drugs that are used clinically. However, few studies focused on the genomic and plasmid profiles of multidrug-resistant\n                        <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                        isolates from pediatric patients. In the present report, our data indicate that\n                        <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                        strains isolated from pediatric patients have different antimicrobial resistance levels and plasmid transferability compared to those from adults. These findings provide crucial insights into pediatric patients\u2019 isolated\n                        <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                        strains from Zhuhai, China, which can be leveraged to optimize drug regimens, leading to more effective and less toxic treatments for pediatric infections, and call for more attention to\n                        <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                        epidemiology and antimicrobial resistance patterns in children.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.04063-25","type":"journal-article","created":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T13:21:58Z","timestamp":1776777718000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Genomic and plasmid profiles of multidrug-resistant\n                    <i>Salmonella<\/i>\n                    isolates from pediatric patients from Zhuhai, China"],"prefix":"10.1128","volume":"14","author":[{"given":"Yongru","family":"Chen","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02bnz8785","id-type":"ROR","asserted-by":"publisher"}],"name":"Clinical Research Center, The First Affiliated Hospital of Shantou University Medical College","place":["Shantou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanmei","family":"Fang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00qzjvm58","id-type":"ROR","asserted-by":"publisher"}],"name":"Zhuhai Center for Disease Control and Prevention","place":["Zhuhai, 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