{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T19:35:29Z","timestamp":1791401729704,"version":"4.3.3"},"reference-count":33,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2025,5,6]],"date-time":"2025-05-06T00:00:00Z","timestamp":1746489600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,5,6]],"date-time":"2025-05-06T00:00:00Z","timestamp":1746489600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Bureau of Science and Information Technology of Guangzhou Municipality | Guangzhou Municipal Science and Technology Project","award":["2023A03J0991"],"award-info":[{"award-number":["2023A03J0991"]}]},{"name":"Bureau of Science and Information Technology of Guangzhou Municipality | Guangzhou Municipal Science and Technology Project","award":["2023A03J0539"],"award-info":[{"award-number":["2023A03J0539"]}]},{"name":"MOST | National Key Research and Development Program of China","award":["2022YFC2304800"],"award-info":[{"award-number":["2022YFC2304800"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,5,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              <jats:italic>Mycobacterium abscessus<\/jats:italic>\n              complex (MABC) is the most common rapidly growing non-tuberculous mycobacterium (NTM) and a significant pathogen responsible for various infections. Subspecies of MABC exhibit differing levels of antibiotic resistance, potentially influencing patient prognosis. In this study, 196 MABC clinical isolates were collected from Guangzhou Chest Hospital and subjected to a drug sensitivity test (DST) followed by whole-genome sequencing. In our study, the most common subspecies was\n              <jats:italic>Mycobacterium abscessus<\/jats:italic>\n              subsp.\n              <jats:italic>abscessus<\/jats:italic>\n              , followed by\n              <jats:italic>M. abscessus<\/jats:italic>\n              subsp.\n              <jats:italic>massiliense<\/jats:italic>\n              , with\n              <jats:italic>M. abscessus<\/jats:italic>\n              subsp.\n              <jats:italic>bolletii<\/jats:italic>\n              being the least frequent. The isolates were most susceptible to tigecycline and amikacin, followed by clarithromycin and linezolid. Bedaquiline and clofazimine showed promising anti-bacterial activity, whereas doramapimod and capreomycin demonstrated limited efficacy. The\n              <jats:italic>erm(41<\/jats:italic>\n              ) gene mutation at position 28 and the\n              <jats:italic>rrl<\/jats:italic>\n              gene at position 2270 were identified in clarithromycin-resistant isolates. Additionally, the\n              <jats:italic>rrs<\/jats:italic>\n              gene mutation at position 1375 may serve as an indicator of amikacin resistance. Regular molecular surveillance investigation of NTM and DST is recommended for improved management.\n            <\/jats:p>\n            <jats:sec>\n              <jats:title>IMPORTANCE<\/jats:title>\n              <jats:p>As Guangdong is a region with a high prevalence of NTM, this study investigates the epidemiological trends of MABC subspecies and their associated drug-resistance profiles, addressing a critical research gap. The findings are crucial for guiding hospital drug management and clinical decision making, enabling more accurate diagnoses and the formulation of personalized, effective treatment plans. Additionally, this study provides a valuable reference for the development of drug-resistance detection reagents and new anti-bacterial agents.<\/jats:p>\n            <\/jats:sec>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.04103-23","type":"journal-article","created":{"date-parts":[[2025,3,19]],"date-time":"2025-03-19T13:00:31Z","timestamp":1742389231000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":7,"title":["Subspecies distribution and drug-resistance characteristics of\n            <i>Mycobacterium abscessus<\/i>\n            complex clinical isolates in South China"],"prefix":"10.1128","volume":"13","author":[{"given":"Xinyu","family":"Wang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Respiratory Disease, Guangzhou Key Laboratory of Tuberculosis Research, Department of Clinical Laboratory, Guangzhou Chest Hospital, Institute of Tuberculosis, Guangzhou Medical University","place":["Guangzhou, China"]},{"name":"Beijing Key Laboratory for Drug Resistant Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biyi","family":"Su","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Respiratory Disease, Guangzhou Key Laboratory of Tuberculosis Research, Department of Clinical Laboratory, Guangzhou Chest Hospital, Institute of Tuberculosis, Guangzhou Medical University","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pinru","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Respiratory Disease, Guangzhou Key Laboratory of Tuberculosis Research, Department of Tuberculosis, Guangzhou Chest Hospital, Institute of Tuberculosis, Guangzhou Medical University","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haobin","family":"Kuang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Respiratory Disease, Guangzhou Key Laboratory of Tuberculosis Research, Department of Tuberculosis, Guangzhou Chest Hospital, Institute of Tuberculosis, Guangzhou Medical University","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Guan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Respiratory Disease, Guangzhou Key Laboratory of Tuberculosis Research, Department of Clinical Laboratory, Guangzhou Chest Hospital, Institute of Tuberculosis, Guangzhou Medical University","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory for Drug Resistant Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liping","family":"Pan","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory for Drug Resistant Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0630-4964","authenticated-orcid":false,"given":"Jialou","family":"Zhu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Respiratory Disease, Guangzhou Key Laboratory of Tuberculosis Research, Department of Clinical Laboratory, Guangzhou Chest Hospital, Institute of Tuberculosis, Guangzhou Medical University","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5219-1265","authenticated-orcid":true,"given":"Yaoju","family":"Tan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Respiratory Disease, Guangzhou Key Laboratory of Tuberculosis Research, Department of Clinical Laboratory, Guangzhou Chest Hospital, Institute of Tuberculosis, Guangzhou Medical University","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chest.2021.10.003"},{"key":"e_1_3_4_3_2","unstructured":"Wang Y. 2011. 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