{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T20:43:47Z","timestamp":1773953027378,"version":"3.50.1"},"reference-count":27,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2024,6,4]],"date-time":"2024-06-04T00:00:00Z","timestamp":1717459200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,6,4]],"date-time":"2024-06-04T00:00:00Z","timestamp":1717459200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2024,6,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              Resistance to ceftazidime-avibactam (CZA) due to\n              <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n              carbapenemase (KPC) variants is increasing worldwide. We characterized two CZA-resistant clinical\n              <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n              strains by antimicrobial susceptibility test, conjugation assays, and WGS. Isolates belonged to ST258 and ST45, and produced a KPC-31 and a novel variant KPC-197, respectively. The novel KPC variant presents a deletion of two amino acids on the \u2126-loop (del_168\u2013169_EL) and an insertion of two amino acids in position 274 (Ins_274_DS). Continued surveillance of KPC variants conferring CZA resistance in Colombia is warranted.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              Latin America and the Caribbean is an endemic region for carbapenemases. Increasingly high rates of\n              <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n              carbapenemase (KPC) have established ceftazidime-avibactam (CZA) as an essential antimicrobial for the treatment of infections due to MDR Gram-negative pathogens. Although other countries in the region have reported the emergence of CZA-resistant KPC variants, this is the first description of such enzymes in Colombia. This finding warrants active surveillance, as dissemination of these variants could have devastating public health consequences.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.04105-23","type":"journal-article","created":{"date-parts":[[2024,5,3]],"date-time":"2024-05-03T13:00:23Z","timestamp":1714741223000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":9,"title":["First report of KPC variants conferring ceftazidime-avibactam resistance in Colombia: introducing KPC-197"],"prefix":"10.1128","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0361-7893","authenticated-orcid":true,"given":"Elsa","family":"De la Cadena","sequence":"first","affiliation":[{"name":"Grupo de Investigaci\u00f3n en Resistencia Antimicrobiana y Epidemiolog\u00eda Hospitalaria, Universidad El Bosque, Bogot\u00e1, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1380-9824","authenticated-orcid":false,"given":"Mar\u00eda Fernanda","family":"Mojica","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n en Resistencia Antimicrobiana y Epidemiolog\u00eda Hospitalaria, Universidad El Bosque, Bogot\u00e1, Colombia"},{"name":"Department of Molecular Biology and Microbiology, School of Medicine, Case Western Reserve University, Cleveland, Ohio, USA"},{"name":"CWRU-Cleveland VAMC Center for Antimicrobial Resistance and Epidemiology (Case VA CARES), Cleveland, Ohio, USA"},{"name":"Research Service, VA Northeast Ohio Healthcare System, Cleveland, Ohio, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4410-1658","authenticated-orcid":false,"given":"Laura J.","family":"Rojas","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Microbiology, School of Medicine, Case Western Reserve University, Cleveland, Ohio, USA"},{"name":"CWRU-Cleveland VAMC Center for Antimicrobial Resistance and Epidemiology (Case VA CARES), Cleveland, Ohio, USA"},{"name":"Research Service, VA Northeast Ohio Healthcare System, Cleveland, Ohio, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Betsy E.","family":"Castro","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n en Resistencia Antimicrobiana y Epidemiolog\u00eda Hospitalaria, Universidad El Bosque, Bogot\u00e1, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan Carlos","family":"Garc\u00eda-Betancur","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n en Resistencia Antimicrobiana y Epidemiolog\u00eda Hospitalaria, Universidad El Bosque, Bogot\u00e1, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven H.","family":"Marshall","sequence":"additional","affiliation":[{"name":"Research Service, VA Northeast Ohio Healthcare System, Cleveland, Ohio, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-9784-6700","authenticated-orcid":true,"given":"Natalia","family":"Restrepo","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n en Resistencia Antimicrobiana y Epidemiolog\u00eda Hospitalaria, Universidad El Bosque, Bogot\u00e1, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nancy Patricia","family":"Castro-Caro","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3n Hospital San Vicente de Paul, Rionegro, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Magalis","family":"Fonseca-Carrillo","sequence":"additional","affiliation":[{"name":"Cl\u00ednica Farallones Christus Sinergia, Cali, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Pallares","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n en Resistencia Antimicrobiana y Epidemiolog\u00eda Hospitalaria, Universidad El Bosque, Bogot\u00e1, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3299-894X","authenticated-orcid":true,"given":"Robert A.","family":"Bonomo","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Microbiology, School of Medicine, Case Western Reserve University, Cleveland, Ohio, USA"},{"name":"CWRU-Cleveland VAMC Center for Antimicrobial Resistance and Epidemiology (Case VA CARES), Cleveland, Ohio, USA"},{"name":"Department of Medicine, Case Western Reserve University School of Medicine, Cleveland, Ohio, USA"},{"name":"Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, Ohio, USA"},{"name":"Department of Biochemistry, Case Western Reserve University School of Medicine, Cleveland, Ohio, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Virginia","family":"Villegas","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n en Resistencia Antimicrobiana y Epidemiolog\u00eda Hospitalaria, Universidad El Bosque, Bogot\u00e1, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(13)70190-7"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.idc.2016.02.007"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciw243"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00574-21"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00890-21"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.01714-21"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02534-16"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/aac.00447-22"},{"key":"e_1_3_2_10_2","volume-title":"CLSI supplement M100Performance standards for antimicrobial susceptibility testing","author":"Clinical and Laboratory Standards Institute (CLSI)","year":"2023","unstructured":"Clinical and Laboratory Standards Institute (CLSI). 2023. CLSI supplement M100. Performance standards for antimicrobial susceptibility testing. 33rd ed. Clinical and Laboratory Standards Institute, Wayne, PA.","edition":"33"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00977-15"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac1003"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.02245-21"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01165-15"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2020.03.013"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.00374-23"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkz156"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/aac.00648-22"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dky082"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jix524"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.7705\/biomedica.v39i3.4351"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciab777"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01775-15"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics9020062"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/msphere.00651-22"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00079-17"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00409-20"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics11050628"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.04105-23","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.04105-23","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,4]],"date-time":"2024-06-04T14:07:48Z","timestamp":1717510068000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.04105-23"}},"subtitle":[],"editor":[{"given":"Karen C.","family":"Carroll","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2024,6,4]]},"references-count":27,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2024,6,4]]}},"alternative-id":["10.1128\/spectrum.04105-23"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.04105-23","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,6,4]]},"assertion":[{"value":"2023-12-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-03-19","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-05-03","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e04105-23"}}