{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T14:48:23Z","timestamp":1791557303878,"version":"4.3.4"},"reference-count":40,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2025,2,4]],"date-time":"2025-02-04T00:00:00Z","timestamp":1738627200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,2,4]],"date-time":"2025-02-04T00:00:00Z","timestamp":1738627200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100014653","name":"NIHR | Manchester Biomedical Research Centre","doi-asserted-by":"crossref","award":["NIHR203308"],"award-info":[{"award-number":["NIHR203308"]}],"id":[{"id":"10.13039\/100014653","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,2,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Coronavirus disease 2019 (COVID-19)-associated pulmonary aspergillosis (CAPA) is a life-threatening complication in patients with severe COVID-19. Previously, acute respiratory distress syndrome in patients with COVID-19 has been associated with lung fungal dysbiosis, evidenced by reduced microbial diversity and\n                      <jats:italic>Candida<\/jats:italic>\n                      colonization. Increased fungal burden in the lungs of critically ill COVID-19 patients is linked to prolonged mechanical ventilation and increased mortality. However, specific mycobiome signatures associated with severe COVID-19 in the context of survival and antifungal drug prophylaxis have not yet been determined, and such knowledge could have an important impact on treatment. To understand the composition of the respiratory mycobiome in critically ill COVID-19 patients with and without CAPA and the impact of antifungal use in patient outcome, we performed a multinational study of 39 COVID-19 patients in intensive care units (ICUs). Respiratory mycobiome was profiled using internal transcribed spacer 1 sequencing, and\n                      <jats:italic>Aspergillus fumigatus<\/jats:italic>\n                      burden was further validated using quantitative PCR. Fungal communities were investigated using alpha diversity, beta diversity, taxa predominance, and taxa abundances. Respiratory mycobiomes of COVID-19 patients were dominated by\n                      <jats:italic>Candida<\/jats:italic>\n                      and\n                      <jats:italic>Aspergillus<\/jats:italic>\n                      . There was no significant association with corticosteroid use or CAPA diagnosis and respiratory fungal communities. Increased\n                      <jats:italic>A. fumigatus<\/jats:italic>\n                      burden was associated with mortality and, the use of azoles at ICU admission was linked with an absence of\n                      <jats:italic>A. fumigatus<\/jats:italic>\n                      . Our findings suggest that mold-active antifungal treatment at ICU admission may be linked with reduced\n                      <jats:italic>A. fumigatus-<\/jats:italic>\n                      associated mortality in severe COVID-19. However, further studies are warranted on this topic.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Invasive fungal infections are a serious complication affecting up to a third of patients with severe COVID-19. Nevertheless, our understanding of the fungal communities in the lungs during critically ill COVID-19 remains limited. Evidence suggests a higher fungal burden is associated with prolonged ventilation and higher mortality, although the particular organisms responsible for this link are unclear. Antifungal prophylaxis may be beneficial for reducing the burden of fungal co-infections in COVID-19 intensive care. However, the composition of the fungal microbiome in severe COVID-19 in relation to prophylactic antifungals, as well as how this is associated with survival outcomes, is yet to be studied. Our study provides insights into the lung fungal microbiome in severe COVID-19 and has found antifungal treatment to be associated with lower\n                        <jats:italic>Aspergillus fumigatus<\/jats:italic>\n                        burden and that higher levels of this pathogen are associated with mortality. Therefore, our study suggests mold-active antifungal prophylaxis may be beneficial in severe COVID-19.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.04110-23","type":"journal-article","created":{"date-parts":[[2024,12,19]],"date-time":"2024-12-19T09:17:02Z","timestamp":1734599822000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":7,"title":["Mycobiome analyses of critically ill COVID-19 patients"],"prefix":"10.1128","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4983-8352","authenticated-orcid":true,"given":"Danielle","family":"Weaver","sequence":"first","affiliation":[{"name":"University of Manchester","place":["Manchester, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7027-4598","authenticated-orcid":true,"given":"Sara","family":"Gago","sequence":"additional","affiliation":[{"name":"University of Manchester","place":["Manchester, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matteo","family":"Bassetti","sequence":"additional","affiliation":[{"name":"Department of Health Sciences (DISSAL), University of Genoa","place":["Genoa, Italy"]},{"name":"Infectious Diseases Unit, IRCCS San Martino Polyclinic Hospital","place":["Genoa, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2385-1759","authenticated-orcid":true,"given":"Daniele Roberto","family":"Giacobbe","sequence":"additional","affiliation":[{"name":"Department of Health Sciences (DISSAL), University of Genoa","place":["Genoa, Italy"]},{"name":"Infectious Diseases Unit, IRCCS San Martino Polyclinic Hospital","place":["Genoa, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juergen","family":"Prattes","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Medical University of Graz","place":["Graz, Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Hoenigl","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Medical University of Graz","place":["Graz, Austria"]},{"name":"Biotech Med","place":["Graz, Austria"]},{"name":"Translational Medical Mycology Research Unit, ECMM Excellence Center for Medical Mycology, Medical University of Graz","place":["Graz, Austria"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florian","family":"Reizine","sequence":"additional","affiliation":[{"name":"Medical Intensive Care Unit, Centre Hospitalier Universitaire de Rennes","place":["Rennes, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6338-3094","authenticated-orcid":true,"given":"H\u00e9l\u00e8ne","family":"Guegan","sequence":"additional","affiliation":[{"name":"CHU Rennes, Inserm, EHESP, Institut de Recherche en Sant\u00e9, Environnement et Travail (IRSET), Universit\u00e9 de Rennes","place":["Rennes, France"]},{"name":"Centre Hospitalier Universitaire de Rennes, Laboratoire de Parasitologie-Mycologie, Centre National de R\u00e9f\u00e9rence Mycoses et Antifongiques-Laboratoire Associ\u00e9 Asp-C, European Excellence Center for Medical Mycology (ECMM)","place":["Rennes, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4974-5607","authenticated-orcid":true,"given":"Jean-Pierre","family":"Gangneux","sequence":"additional","affiliation":[{"name":"CHU Rennes, Inserm, EHESP, Institut de Recherche en Sant\u00e9, Environnement et Travail (IRSET), Universit\u00e9 de Rennes","place":["Rennes, France"]},{"name":"Centre Hospitalier Universitaire de Rennes, Laboratoire de Parasitologie-Mycologie, Centre National de R\u00e9f\u00e9rence Mycoses et Antifongiques-Laboratoire Associ\u00e9 Asp-C, European Excellence Center for Medical Mycology (ECMM)","place":["Rennes, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7611-0201","authenticated-orcid":true,"given":"Michael John","family":"Bromley","sequence":"additional","affiliation":[{"name":"University of Manchester","place":["Manchester, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1083-9286","authenticated-orcid":true,"given":"Paul","family":"Bowyer","sequence":"additional","affiliation":[{"name":"University of Manchester","place":["Manchester, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_5_2_2","unstructured":"World Health Organisation. 2024 WHO coronavirus dashboard. 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