{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T17:30:52Z","timestamp":1784827852023,"version":"3.55.0"},"reference-count":32,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T00:00:00Z","timestamp":1722902400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T00:00:00Z","timestamp":1722902400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"MOST | National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["U21A20245, 32272830"],"award-info":[{"award-number":["U21A20245, 32272830"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Financial Resourced Program of Chongqing","award":["23509J"],"award-info":[{"award-number":["23509J"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2024,8,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              Gut bacteria belonging to the\n              <jats:italic>Clostridium<\/jats:italic>\n              family play a pivotal role in regulating host energy balance and metabolic homeostasis. As a commensal bacterium,\n              <jats:italic>Clostridium sporogenes<\/jats:italic>\n              has been implicated in modulating host energy homeostasis, albeit the underlying mechanism remains elusive. Therefore, this study aimed to investigate the impact of\n              <jats:italic>C. sporogenes<\/jats:italic>\n              supplementation on various physiological parameters, intestinal morphology, particularly adipose tissue accumulation, and glucolipid metabolism in mice. The findings reveal that mice supplemented with\n              <jats:italic>C. sporogenes<\/jats:italic>\n              for 6 weeks exhibited a notable increase in body weight, fat mass, adipocyte size, and serum triglyceride (TG) levels. Notably, the increased fat accumulation is observed despite consistent feed intake in treated mice. Mechanistically,\n              <jats:italic>C. sporogenes<\/jats:italic>\n              supplementation significantly improved the structure integrity of intestinal villi and enhanced energy absorption efficiency while reducing excretion of carbohydrates and fatty acids in feces. This was accompanied by upregulation of glucose and fatty acid transporter expression. Furthermore, supplementation with\n              <jats:italic>C. sporogenes<\/jats:italic>\n              promoted adipogenesis in both liver and adipose tissues, as evidenced by increased levels of hepatic pyruvate, acetyl-CoA, and TG, along with elevated expression levels of genes associated with lipid synthesis. Regarding the microbiological aspect,\n              <jats:italic>C. sporogenes<\/jats:italic>\n              supplementation correlated with an increased abundance of\n              <jats:italic>Clostridium<\/jats:italic>\n              genus bacteria and enhanced carbohydrate enzyme activity. In summary,\n              <jats:italic>C. sporogenes<\/jats:italic>\n              supplementation significantly promotes fat accumulation in mice by augmenting energy absorption and adipogenesis, possibly mediated by the expansion of\n              <jats:italic>Clostridium<\/jats:italic>\n              bacteria population with robust glycolipid metabolic ability.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              The\n              <jats:italic>Clostridia<\/jats:italic>\n              clusters have been implicated in energy metabolism, the specific species and underlying mechanisms remain unclear. This present study is the first to report\n              <jats:italic>Clostridium sporogenes<\/jats:italic>\n              is able to affect fat accumulation and glycolipid metabolism. We indicated that gavage of\n              <jats:italic>C. sporogenes<\/jats:italic>\n              promoted the adipogenesis and fat accumulation in mice by not only increasing the abundance of\n              <jats:italic>Clostridium<\/jats:italic>\n              bacteria but by also enhancing the metabolic absorption of carbohydrates and fatty acids significantly. Obviously, changes of gut microbiota caused by the\n              <jats:italic>C. sporogenes<\/jats:italic>\n              , especially the significant increase of\n              <jats:italic>Clostridium<\/jats:italic>\n              bacteria, contributed to the fat accumulation of mice. In addition, the enhancement of\n              <jats:italic>Clostridium<\/jats:italic>\n              genus bacteria remarkably improved the synthesis of hepatic pyruvate, acetyl-CoA, and triglyceride levels, as well as reduced the excretion of fecal carbohydrates, short-chain fatty acids, and free fatty acids remarkably. These findings will help us to understand the relationship of specific bacteria and host energy homeostasis.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.04116-23","type":"journal-article","created":{"date-parts":[[2024,6,25]],"date-time":"2024-06-25T13:01:32Z","timestamp":1719320492000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["<i>Clostridium sporogenes<\/i>\n            increases fat accumulation in mice by enhancing energy absorption and adipogenesis"],"prefix":"10.1128","volume":"12","author":[{"given":"Lei","family":"Du","sequence":"first","affiliation":[{"name":"Chongqing Academy of Animal Science, Chongqing, China"},{"name":"Zhejiang Engineering Research Center for Tissue Repair Materials, Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, Zhejiang, 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