{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T01:07:48Z","timestamp":1781658468897,"version":"3.54.5"},"reference-count":50,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2024,7,2]],"date-time":"2024-07-02T00:00:00Z","timestamp":1719878400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,7,2]],"date-time":"2024-07-02T00:00:00Z","timestamp":1719878400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2024,7,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              In this study, we investigated the prevalence of\n              <jats:italic>Bartonella<\/jats:italic>\n              in deer from Qilian County, Qinghai Province, China. Blood samples were collected from 69 red deer, 40 white-lipped deer, and 27 sika deer. The detection of\n              <jats:italic>Bartonella<\/jats:italic>\n              spp. has been conducted. The overall prevalence of\n              <jats:italic>Bartonella<\/jats:italic>\n              was 33.6% (46\/135). Species-specific prevalence was 50.72% in red deer (35\/69), 20.00% in white-lipped deer (8\/40), and 11.11% in sika deer (3\/27). There were significant differences in the prevalence rates among the different species of deer. The amplicon sequence comparison revealed a high homology of the ruminant-associated\n              <jats:italic>Bartonella<\/jats:italic>\n              spp. Nanopore sequencing further confirmed the results.\n              <jats:italic>Bartonella<\/jats:italic>\n              reads were presented in each of the qPCR-positive samples. Phylogenetic analysis indicated that the\n              <jats:italic>Bartonella<\/jats:italic>\n              sequences detected in deer blood were closely related to ruminant-borne\n              <jats:italic>Bartonella<\/jats:italic>\n              spp. In summary, we reported the\n              <jats:italic>Bartonella<\/jats:italic>\n              prevalence of different deer species in Qinghai, and there were at least one species of ruminant-associated\n              <jats:italic>Bartonella<\/jats:italic>\n              ,\n              <jats:italic>B. schoenbuchensis<\/jats:italic>\n              .\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              This is the first report about\n              <jats:italic>Bartonella<\/jats:italic>\n              infections in the deer population from China. We found that there were two species of\n              <jats:italic>Bartonella<\/jats:italic>\n              and an unidentified species of\n              <jats:italic>Bartonella<\/jats:italic>\n              among the unculturing strains carried by these deer populations. We first used Nanopore sequencing to detect\n              <jats:italic>Bartonella<\/jats:italic>\n              from deer blood samples and indicated that Nanopore sequencing is beneficial to detect pathogens due to its advantage of real-time and high sensitivity.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.04120-23","type":"journal-article","created":{"date-parts":[[2024,5,24]],"date-time":"2024-05-24T13:01:23Z","timestamp":1716555683000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":4,"title":["Detection of\n            <i>Bartonella<\/i>\n            spp. in farmed deer (Artiodactyla: Cervidae) using multiplex assays in the Qinghai-Tibet Plateau, China"],"prefix":"10.1128","volume":"12","author":[{"given":"Yu","family":"Miao","sequence":"first","affiliation":[{"name":"National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wentao","family":"Guo","sequence":"additional","affiliation":[{"name":"Qinghai Provincial Institute of Endemic Disease Control and Prevention, Xining, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0109-2085","authenticated-orcid":true,"given":"Wen","family":"Zhang","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhizheng","family":"Chen","sequence":"additional","affiliation":[{"name":"Qilian County Center for Disease Control and Prevention, Haibei Tibetan Autonomous Prefecture, Qilian, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Delan","family":"Mian","sequence":"additional","affiliation":[{"name":"Qilian County Center for Disease Control and Prevention, Haibei Tibetan Autonomous Prefecture, Qilian, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruixiao","family":"Li","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China"},{"name":"School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ailing","family":"Xu","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Chen","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0757-4264","authenticated-orcid":true,"given":"Dongmei","family":"Li","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(01)02176-X"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1203.050931"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.13.3.428"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2915.2008.00713.x"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cimid.2011.04.005"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature06536"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/ccr3.7561"},{"key":"e_1_3_2_9_2","volume-title":"Infectious Diseases Society of America 2005 Annual Meeting","author":"Lescano A","year":"2005","unstructured":"Lescano A, Tarazona A, Smoak B, Maguina C, Laughlin L. 2005. 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