{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T16:22:16Z","timestamp":1759940536749,"version":"3.41.2"},"reference-count":17,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2024,4,2]],"date-time":"2024-04-02T00:00:00Z","timestamp":1712016000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,4,2]],"date-time":"2024-04-02T00:00:00Z","timestamp":1712016000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2024,4,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              We conducted a comparative evaluation of the FDA-cleared Simplexa GBS Direct and ARIES GBS molecular assays for the detection of\n              <jats:italic>Streptococcus agalactiae<\/jats:italic>\n              (Group B\n              <jats:italic>Streptococcus<\/jats:italic>\n              , GBS) in 386 prospectively collected, broth-enriched vaginal\/rectal swab specimens. The sensitivity of each test was 96.2% and specificity was \u226598.7% when compared to a combined direct and enriched culture method using chromogenic culture medium. A total of four specimens were called positive by both molecular assays but negative by culture, likely representing specimens with a low burden of GBS in these specimens. Two specimens were reported positive by culture but negative by both molecular assays. One of these specimens demonstrated atypically colored colonies on chromogenic agar; the other yielded typically colored colonies only observed after broth enrichment. Our data demonstrate equivalent performance of Simplexa and ARIES molecular assays for the detection of GBS in clinical specimens.\n            <\/jats:p>\n            <jats:sec>\n              <jats:title>IMPORTANCE<\/jats:title>\n              <jats:p>Clinical laboratories often face decisions regarding which of the multiple available molecular platforms would best fit their needs based on cost, workflow, menu, and diagnostic performance. Therefore, objective clinical comparisons of similar molecular tests are valuable resources to aid these decisions. We provide a clinical comparison of two FDA-cleared tests to routine culture and to each other that can be used by clinical laboratories when determining which of the available molecular platforms would best fit their laboratory in terms of workflow, cost, and performance.<\/jats:p>\n            <\/jats:sec>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.04164-23","type":"journal-article","created":{"date-parts":[[2024,3,5]],"date-time":"2024-03-05T15:02:35Z","timestamp":1709650955000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["Comparison of the Simplexa GBS Direct and ARIES GBS assays for the detection of\n            <i>S. agalactiae<\/i>\n            in broth-enriched swab specimens"],"prefix":"10.1128","volume":"12","author":[{"given":"Lavannya","family":"Sabharwal","sequence":"first","affiliation":[{"name":"Department of Pathology, The Medical College of Wisconsin, Milwaukee, Wisconsin, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew L.","family":"Faron","sequence":"additional","affiliation":[{"name":"Department of Pathology, The Medical College of Wisconsin, Milwaukee, Wisconsin, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4111-048X","authenticated-orcid":true,"given":"Blake W.","family":"Buchan","sequence":"additional","affiliation":[{"name":"Department of Pathology, The Medical College of Wisconsin, Milwaukee, Wisconsin, USA"},{"name":"Children\u2019s Wisconsin, Milwaukee, Wisconsin, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2021.720789"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms10122483"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.00437"},{"key":"e_1_3_1_5_2","first-page":"1","article-title":"Prevention of perinatal group B streptococcal disease--revised guidelines from CDC, 2010","volume":"59","author":"Verani JR","year":"2010","unstructured":"Verani JR, McGee L, Schrag SJ, Division of Bacterial Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention (CDC). 2010. Prevention of perinatal group B streptococcal disease--revised guidelines from CDC, 2010. MMWR Recomm Rep 59:1\u201336.","journal-title":"MMWR Recomm Rep"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01902-19"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02598-14"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01768-17"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01081-14"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.8.2298-2300.1998"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00261-15"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0029-7844(95)00402-5"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00043-17"},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02926-13"},{"key":"e_1_3_1_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/jam.14331"},{"key":"e_1_3_1_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01230-20"},{"key":"e_1_3_1_18_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2705.203428"},{"key":"e_1_3_1_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00802-09"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.04164-23","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.04164-23","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,2]],"date-time":"2024-04-02T14:59:04Z","timestamp":1712069944000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.04164-23"}},"subtitle":[],"editor":[{"given":"Paul M.","family":"Luethy","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2024,4,2]]},"references-count":17,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2024,4,2]]}},"alternative-id":["10.1128\/spectrum.04164-23"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.04164-23","relation":{},"ISSN":["2165-0497"],"issn-type":[{"type":"electronic","value":"2165-0497"}],"subject":[],"published":{"date-parts":[[2024,4,2]]},"assertion":[{"value":"2023-12-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-03-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e04164-23"}}