{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:56:15Z","timestamp":1791294975299,"version":"4.1.0"},"reference-count":26,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32470193"],"award-info":[{"award-number":["32470193"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Nankai University Tianjin Applied and Fundamental Research Project","award":["22JCZDJC0041"],"award-info":[{"award-number":["22JCZDJC0041"]}],"id":[{"id":"https:\/\/ror.org\/01y1kjr75","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Natural Science Foundation of Tianjin Municipality","award":["22JCYBJC00790"],"award-info":[{"award-number":["22JCYBJC00790"]}]},{"name":"Natural Science Foundation of Tianjin Municipality","award":["22JCYBJC00560"],"award-info":[{"award-number":["22JCYBJC00560"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2026,10,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Dequalinium chloride is a dicationic amphiphilic quaternary ammonium compound that is used as an anti-infective drug. Here, we found that it potentiates bactericidal efficacies of aminoglycoside antibiotics against\n                      <jats:italic toggle=\"yes\">Pseudomonas aeruginosa<\/jats:italic>\n                      . Dequalinium chloride increases respiratory activity and bacterial membrane potential, thereby promoting intracellular uptake of aminoglycosides. Furthermore, the dequalinium chloride\u2013amikacin combination demonstrated a synergistic bactericidal effect in a murine acute pneumonia model, indicating its therapeutic potential.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Antimicrobial resistance (AMR) poses a severe global health threat. Novel antibiotics and therapeutic strategies are urgently needed. This study revealed that dequalinium chloride (DC) synergizes with aminoglycosides against planktonic and biofilm-associated\n                        <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                        , which is an important pathogen. Mechanistic study reveals that DC enhances bacterial respiration and membrane potential to promote aminoglycosides uptake. The DC\u2013amikacin combination is effective in a murine pneumonia model. These findings provide a promising therapeutic strategy for combating\n                        <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                        infections.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.04183-25","type":"journal-article","created":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T13:00:36Z","timestamp":1788958836000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Dequalinium chloride promotes the killing efficacy of aminoglycoside antibiotics against\n                    <i>Pseudomonas aeruginosa<\/i>\n                    by enhancing membrane potential"],"prefix":"10.1128","volume":"14","author":[{"given":"Xiaoya","family":"Wei","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01y1kjr75","id-type":"ROR","asserted-by":"publisher"}],"name":"State Key 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