{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T01:15:47Z","timestamp":1788225347925,"version":"build-2803163510"},"reference-count":24,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T00:00:00Z","timestamp":1783382400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T00:00:00Z","timestamp":1783382400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Science and Technology Committee of Tianjin","award":["22JCYBJC00790"],"award-info":[{"award-number":["22JCYBJC00790"]}]},{"name":"Science and Technology Committee of Tianjin","award":["22JCYBJC00560"],"award-info":[{"award-number":["22JCYBJC00560"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32170177, 32470193"],"award-info":[{"award-number":["32170177, 32470193"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32170199"],"award-info":[{"award-number":["32170199"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Fundamental Research Funds for the Central Universities, Nankai University","award":["63231048"],"award-info":[{"award-number":["63231048"]}]},{"name":"Fundamental Research Funds for the Central Universities, Nankai University","award":["63231047"],"award-info":[{"award-number":["63231047"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2026,7,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      The type III secretion system (T3SS) is a major virulence determinant in\n                      <jats:italic toggle=\"yes\">Pseudomonas aeruginosa<\/jats:italic>\n                      , which is activated by the master regulator ExsA.\n                      <jats:italic toggle=\"yes\">exsA<\/jats:italic>\n                      is transcribed from two distinct promoters: its operon promoter P\n                      <jats:italic toggle=\"yes\">\n                        <jats:sub>exsC<\/jats:sub>\n                      <\/jats:italic>\n                      and its own promoter P\n                      <jats:italic toggle=\"yes\">\n                        <jats:sub>exsA<\/jats:sub>\n                      <\/jats:italic>\n                      . Here, we identify PA5201, a Tex-family protein, as a novel repressor of T3SS in\n                      <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                      . PA5201 directly binds to the P\n                      <jats:italic toggle=\"yes\">\n                        <jats:sub>exsC<\/jats:sub>\n                      <\/jats:italic>\n                      promoter and represses its activity. Deletion of\n                      <jats:italic toggle=\"yes\">PA5201<\/jats:italic>\n                      increased T3SS expression, bacterial cytotoxicity, and bacterial load in a mouse acute pneumonia model, whereas overexpression of\n                      <jats:italic toggle=\"yes\">PA5201<\/jats:italic>\n                      decreased T3SS expression. Reporter assays and western blot analysis further revealed that PA5201-mediated repression of P\n                      <jats:italic toggle=\"yes\">\n                        <jats:sub>exsC<\/jats:sub>\n                      <\/jats:italic>\n                      is independent of ExsA and does not occur at the post-transcriptional level. Electrophoretic mobility shift assay (EMSA) showed that PA5201 binds to the region between \u221272 and \u221245 bp relative to the\n                      <jats:italic toggle=\"yes\">exsC<\/jats:italic>\n                      start codon. Collectively, this study identifies PA5201 as a novel T3SS repressor and elucidates its molecular mechanism in\n                      <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                      .\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        <jats:italic toggle=\"yes\">Pseudomonas aeruginosa<\/jats:italic>\n                        is an important opportunistic human pathogen responsible for a wide range of infections, particularly in immunocompromised individuals. The type III secretion system (T3SS) plays a critical role in acute\n                        <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                        infections. Here, we identify PA5201, a previously uncharacterized Tex-family regulator, as a novel transcriptional repressor of T3SS. We demonstrate that PA5201 directly binds to and represses the P\n                        <jats:italic toggle=\"yes\">\n                          <jats:sub>exsC<\/jats:sub>\n                        <\/jats:italic>\n                        promoter. This study reveals a new regulator of T3SS in\n                        <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                        and clarifies the molecular mechanism of PA5201-mediated T3SS regulation.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.04189-25","type":"journal-article","created":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T13:00:53Z","timestamp":1781269253000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["PA5201 represses type III secretion system by binding to the P\n                    <i>\n                      <sub>exsC<\/sub>\n                    <\/i>\n                    promoter in\n                    <i>Pseudomonas aeruginosa<\/i>"],"prefix":"10.1128","volume":"14","author":[{"given":"Lepeng","family":"Wang","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01y1kjr75","id-type":"ROR","asserted-by":"publisher"}],"name":"State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai University","place":["Tianjin, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenyu","family":"Shen","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01y1kjr75","id-type":"ROR","asserted-by":"publisher"}],"name":"State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai University","place":["Tianjin, 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