{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T14:45:11Z","timestamp":1789397111269,"version":"build-2803163510"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2024,6,4]],"date-time":"2024-06-04T00:00:00Z","timestamp":1717459200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,6,4]],"date-time":"2024-06-04T00:00:00Z","timestamp":1717459200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"MOST | National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32373074"],"award-info":[{"award-number":["32373074"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Innovative research Team in the university of Henan province","award":["23IRTSTHN021"],"award-info":[{"award-number":["23IRTSTHN021"]}]},{"name":"Key projects of the Natural Science Foundation of Henan province","award":["232300421111"],"award-info":[{"award-number":["232300421111"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2024,6,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              The interaction between coexisting plasmids can affect plasmid-carried resistance gene persistence and spread. However, whether the persistence of the\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>CTX-M<\/jats:sub>\n              gene in clinical Enterobacteriaceae is related to the interaction of coresident nonresistance-conferring plasmids has not been reported. This study was initiated to elucidate how a nonresistance-conferring IncI1 plasmid affected the\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>CTX-M<\/jats:sub>\n              -bearing IncFII plasmid colocated on the same cell. Herein, we constructed three isogenic derivatives of\n              <jats:italic>E. coli<\/jats:italic>\n              C600, designated as C600\n              <jats:sub>FII<\/jats:sub>\n              , C600\n              <jats:sub>I1<\/jats:sub>\n              , and C600\n              <jats:sub>FII+I1<\/jats:sub>\n              , which harbored the\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>CTX-M<\/jats:sub>\n              -IncFII plasmid and\/or the nonresistance-IncI1 one. We discovered that strain C600\n              <jats:sub>FII+I1<\/jats:sub>\n              conferred higher fitness advantages than strain C600\n              <jats:sub>FII<\/jats:sub>\n              ; also, the stability of the\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>CTX-M<\/jats:sub>\n              -IncFII plasmid was noticeably improved in an antibiotic-free environment when it coexisted with the IncI1 plasmid. To further explore why the IncI1 plasmid enhanced the persistence of the\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>CTX-M<\/jats:sub>\n              -IncFII plasmid, we assessed the\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>CTX-M<\/jats:sub>\n              -IncFII plasmid's copy numbers, conjugation frequencies, and\n              <jats:italic>rep<\/jats:italic>\n              gene expressions in strains C600\n              <jats:sub>FII<\/jats:sub>\n              and C600\n              <jats:sub>FII+I1<\/jats:sub>\n              . The results demonstrated that the\n              <jats:italic>rep<\/jats:italic>\n              expressions of the\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>CTX-M<\/jats:sub>\n              -IncFII plasmid in strain C600\n              <jats:sub>FII+I1<\/jats:sub>\n              was greatly decreased, along with the plasmid\u2019s copy numbers and mating efficiencies, compared to those in strain C600\n              <jats:sub>FII<\/jats:sub>\n              . Moreover, further study revealed that the intracellular ATP levels of strain C600\n              <jats:sub>FII+I1<\/jats:sub>\n              were far lower than those of strain C600\n              <jats:sub>FII<\/jats:sub>\n              . Our findings confirmed that coexistence of the nonresistance-IncI1 plasmid can keep the\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>CTX-M<\/jats:sub>\n              -IncFII plasmid more stable by increasing the fitness advantages of the host bacteria, which will pose a threat to preventing the long-term presence of the plasmid-carried\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>CTX-M<\/jats:sub>\n              gene in clinical Enterobacteriaceae.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              So far, plasmid-carried\n              <jats:italic>bla<\/jats:italic>\n              <jats:sc>\n                <jats:sub>CTX-M<\/jats:sub>\n              <\/jats:sc>\n              is still the most common extended-spectrum beta-lactamase (ESBL) genotype in clinical settings worldwide. Except for the widespread use of third-generation cephalosporins, the interaction between coexisting plasmids can also affect the long-term stable existence of the\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>CTX-M<\/jats:sub>\n              gene; however, the study on that is still sparse. In the present study, we assess the interaction of coinhabitant plasmids\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>CTX-M<\/jats:sub>\n              -IncFII and nonresistance-IncI1. Our results confirmed that the increased fitness advantages of strain C600\n              <jats:sub>FII+I1<\/jats:sub>\n              were attributable to the cohabitant nonresistance-IncI1 plasmid, which largely reduced the intracellular ATP levels of host bacteria, thus decreasing the rep gene expression of the\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>CTX-M<\/jats:sub>\n              -IncFII plasmid, its copy numbers, and mating efficiencies, while the higher fitness advantages of strain C600\n              <jats:sub>FII+I1<\/jats:sub>\n              enhanced the persistence of the\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>CTX-M<\/jats:sub>\n              -IncFII plasmid. The results indicate that the nonresistance-IncI1 plasmid contributes to the long-term existence of the\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>CTX-M<\/jats:sub>\n              -IncFII plasmid, implying a potentially new strategy for controlling the spread of resistance plasmids in clinical settings by targeting nonresistance plasmids.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.04240-23","type":"journal-article","created":{"date-parts":[[2024,4,30]],"date-time":"2024-04-30T13:02:26Z","timestamp":1714482146000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":4,"title":["Coexistence of a nonresistance-conferring IncI1 plasmid favors persistence of the\n            <i>bla<\/i>\n            <sub>CTX-M<\/sub>\n            -bearing IncFII plasmid in\n            <i>Escherichia coli<\/i>"],"prefix":"10.1128","volume":"12","author":[{"given":"Kun","family":"He","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayu","family":"Lin","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yulei","family":"Liang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junling","family":"Cui","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiuru","family":"Chen","sequence":"additional","affiliation":[{"name":"College 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