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Quinolones exert their antibacterial activity by inhibiting DNA gyrase, but most of the isolates acquire quinolone resistance via an amino acid substitution in the A subunit of DNA gyrase. WQ-3810 is a quinolone antibiotic that has been reported to have high potency even to DNA gyrase with amino acid substitutions in several bacterial species; however, there was no information on\n              <jats:italic>C. jejuni<\/jats:italic>\n              . Hence, this study aimed to evaluate the activity of WQ-3810 to inhibit wild-type\/mutant DNA gyrases of\n              <jats:italic>C. jejuni<\/jats:italic>\n              and the bacterial growth for accessing the potency for the treatment of quinolone-resistant\n              <jats:italic>C. jejuni<\/jats:italic>\n              infection. The inhibitory activity of WQ-3810 was assessed and compared with ciprofloxacin and nalidixic acid by calculating the half maximal inhibitory concentration (IC\n              <jats:sub>50<\/jats:sub>\n              ) against wild-type\/mutant DNA gyrases. Next, the minimum inhibitory concentration (MIC) of WQ-3810 and five other quinolones was determined for\n              <jats:italic>C. jejuni<\/jats:italic>\n              including quinolone-resistant strains with amino acid substitutions in GyrA. Furthermore, the interaction between WQ-3810 and wild-type\/mutant DNA gyrase was speculated using docking simulations. The IC\n              <jats:sub>50<\/jats:sub>\n              of WQ-3810 against wild-type DNA gyrase was 1.03 \u00b5g\/mL and not different from that of ciprofloxacin. However, those of WQ-3810 against mutant DNA gyrases were much lower than ciprofloxacin. The MICs of WQ-3810 ranged &lt;0.016\u20130.031 \u00b5g\/mL and were the lowest against both quinolone-susceptible and quinolone-resistant strains among the examined quinolones. The results obtained by the docking simulation agreed well with this observation. WQ-3810 seems to be a promising antimicrobial agent for the infections caused by quinolone-resistant\n              <jats:italic>C. jejuni<\/jats:italic>\n              .\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              WQ-3810, a relatively new quinolone antibiotic, demonstrates exceptional antibacterial properties against certain pathogens in previous studies. However, its efficacy against quinolone-resistant\n              <jats:italic>Campylobacter jejuni<\/jats:italic>\n              was not previously reported. The prevalence of quinolone-resistant\n              <jats:italic>C. jejuni<\/jats:italic>\n              as a cause of foodborne illnesses is increasing, prompting this investigation into the effectiveness of WQ-3810 as a countermeasure. This study revealed high inhibitory activity of WQ-3810 against both wild-type and mutant DNA gyrases of\n              <jats:italic>C. jejuni<\/jats:italic>\n              . WQ-3810 was equally efficacious as ciprofloxacin against wild-type DNA gyrases but showed superior effectiveness against mutant DNA gyrases. WQ-3810 also demonstrated the lowest minimum inhibitory concentrations, highlighting its enhanced potency against both susceptible and resistant strains of\n              <jats:italic>C. jejuni<\/jats:italic>\n              . This observation was well supported by the results of the in silico analysis. Consequently, WQ-3810 exhibits a higher level of bactericidal activity compared to existing quinolones in combating both susceptible and resistant\n              <jats:italic>C. jejuni<\/jats:italic>\n              isolates.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.04322-23","type":"journal-article","created":{"date-parts":[[2024,8,20]],"date-time":"2024-08-20T13:05:01Z","timestamp":1724159101000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["WQ-3810, a fluoroquinolone with difluoropyridine derivative as the R1 group exerts high potency against quinolone-resistant\n            <i>Campylobacter jejuni<\/i>"],"prefix":"10.1128","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9840-3689","authenticated-orcid":true,"given":"Kentaro","family":"Koide","sequence":"first","affiliation":[{"name":"Division of Bioresources, Hokkaido University International Institute for Zoonosis Control, Sapporo, Japan"},{"name":"Department of Bacteriology II, National Institute of Infectious Diseases, Musashimurayama, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Bacteriology II, National Institute of Infectious Diseases, Musashimurayama, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew V. 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