{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T03:40:04Z","timestamp":1788925204789,"version":"build-2803163510"},"reference-count":43,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2023,6,15]],"date-time":"2023-06-15T00:00:00Z","timestamp":1686787200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,6,15]],"date-time":"2023-06-15T00:00:00Z","timestamp":1686787200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000030","name":"HHS | Centers for Disease Control and Prevention","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000030","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2023,6,15]]},"abstract":"<jats:p>The Nanopore MinION-based influenza sequencing approach we are proposing makes it possible to sequence the influenza A virus, irrespective of its diverse serotypes, directly from clinical and environmental swab samples, so that we are not limited to virus culture. This third-generation, portable, multiplexing, and real-time sequencing strategy is highly convenient for local sequencing, particularly in low- and middle-income countries like Bangladesh.<\/jats:p>","DOI":"10.1128\/spectrum.04946-22","type":"journal-article","created":{"date-parts":[[2023,5,22]],"date-time":"2023-05-22T13:00:35Z","timestamp":1684760435000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["Culture-Independent Workflow for Nanopore MinION-Based Sequencing of Influenza A Virus"],"prefix":"10.1128","volume":"11","author":[{"given":"Mojnu","family":"Miah","sequence":"first","affiliation":[{"name":"Infectious Diseases Division, ICDDR,B, Dhaka, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5511-1857","authenticated-orcid":true,"given":"Mohammad Enayet","family":"Hossain","sequence":"additional","affiliation":[{"name":"Infectious Diseases Division, ICDDR,B, Dhaka, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rashedul","family":"Hasan","sequence":"additional","affiliation":[{"name":"Infectious Diseases Division, ICDDR,B, Dhaka, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md Shaheen","family":"Alam","sequence":"additional","affiliation":[{"name":"Infectious Diseases Division, ICDDR,B, Dhaka, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joynob Akter","family":"Puspo","sequence":"additional","affiliation":[{"name":"Infectious Diseases Division, ICDDR,B, Dhaka, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md Mahmudul","family":"Hasan","sequence":"additional","affiliation":[{"name":"Infectious Diseases Division, ICDDR,B, Dhaka, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ariful","family":"Islam","sequence":"additional","affiliation":[{"name":"EcoHealth Alliance, New York, New York, USA"},{"name":"Centre for Integrative Ecology, School of Life and Environmental Science, Deakin University, Burwood, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sukanta","family":"Chowdhury","sequence":"additional","affiliation":[{"name":"Infectious Diseases Division, ICDDR,B, Dhaka, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed Ziaur","family":"Rahman","sequence":"additional","affiliation":[{"name":"Infectious Diseases Division, ICDDR,B, Dhaka, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(17)33293-2"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.3390\/tropicalmed4040138"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-8678-1_2"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiy335"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciz020"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.02748"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1122438"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.10.8851-8856.1999"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0066129"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2015.04.019"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01109-09"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2006.03.027"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/s007050170002"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbt.2012.11.012"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature04239"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2013.07.001"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-0346-8_6"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0023400"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-015-1284-z"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-020-05367-y"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00500-19"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1755-0998.2011.03024.x"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-13-341"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2018.23.50.1800228"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-43524-9"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-32615-8"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/MRA.00912-21"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00766"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02127-19"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00963-19"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02636-10"},{"key":"e_1_3_3_33_2","first-page":"1164","article-title":"Development of multiplex RT-PCR assays for rapid detection and subtyping of influenza type A viruses from clinical specimens","volume":"18","author":"Chang H-K","year":"2008","unstructured":"Chang H-K, Park J-H, Song M-S, Oh T-K, Kim S-Y, Kim C-J, Kim H-G, Sung M-H, Han H-S, Hahn Y-S. 2008. Development of multiplex RT-PCR assays for rapid detection and subtyping of influenza type A viruses from clinical specimens. J Microbiol Biotechnol 18:1164\u20131169.","journal-title":"J Microbiol Biotechnol"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2022.2048971"},{"key":"e_1_3_3_35_2","unstructured":"World Health Organization. 2021. Human infection with avian influenza A (H5N8)\u2014the Russian Federation. https:\/\/web.archive.org\/web\/20210226125105\/https:\/\/www.who.int\/csr\/don\/26-feb-2021-influenza-a-russian-federation\/en\/. Retrieved 26 February 2022."},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0020592"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.4060"},{"key":"e_1_3_3_38_2","unstructured":"Hossain ME Miah M Hasan R Hasan MM Ami JQ Davis W Chowdhury S Rahman MZ. 2022. Detection of a novel highly pathogenic avian influenza (A\/H5N1) virus subclade 2.3.4.4B in a duck in Bangladesh. Abstract ID: AOXI0330. Options XI for the control of influenza Belfast United Kingdom 26\u201329 September."},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/Spectrum.00545-21"},{"key":"e_1_3_3_40_2","unstructured":"World Health Organization. 2009. CDC protocol of real-time RT-PCR for swine influenza A(H1N1). https:\/\/www.who.int\/csr\/resources\/publications\/swineflu\/CDCRealtimeRTPCR_SwineH1Assay-2009_20090430.pdf. Accessed 24 October 2022."},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty191"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1093\/gigascience\/giab008"},{"key":"e_1_3_3_43_2","unstructured":"Li H. 2012. Seqtk. Toolkit for processing sequences in FASTA\/Q formats. https:\/\/github.com\/lh3\/seqtk."},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw857"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.04946-22","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.04946-22","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,6,15]],"date-time":"2023-06-15T13:53:14Z","timestamp":1686837194000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.04946-22"}},"subtitle":[],"editor":[{"given":"Day-Yu","family":"Chao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2023,6,15]]},"references-count":43,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2023,6,15]]}},"alternative-id":["10.1128\/spectrum.04946-22"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.04946-22","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,6,15]]},"assertion":[{"value":"2022-12-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-05-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-05-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e04946-22"}}